Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 31 for “"Inner Cell Mass"”.
-
The Role of Amino Acids and the Threonine-SAM Pathway in the Development of Bovine Inner Cell Mass and Pluripotency
Pluripotent stem cells (PSCs) are the foundation of all cell types in the body including functional gametes. They are derived either from the inner cell mass (ICM) of preimplantation mouse and rat embryos, known as embryonic pluripotent stem cells (ePSCs) or embryonic stem cells (ESCs) or via …
-
Modelling Physical Mechanisms Driving Tissue Self-Organisation in the Early Mammalian Embryo
… 3.5 and 4.5 days after fertilisation, the cells of the inner cell mass evolve from a uniform aggregate to an ordered structure with two distinct tissue layers - the primitive endoderm and epiblast. It was originally assumed that cells differentiated to form these layers in situ, but more …
-
HECAT5 regulates cell proliferation via interaction with M3R in cell type-specific manner
Embryonic stem (ES) cells are derived from pluripotent inner cell mass of the early mammalian embryo. Their self-renewal and pluripotency make them attractive sources for stem cell therapies. For ethical and practical reasons, ES cells have a major threat limiting in potential therapeutic …
-
The Role of Mga in the Survival of Pluripotent Cells During Peri-implantation Development
… vitro blastocyst culture and embryonic stem (ES) cell culture identify a lack of pluripotent inner cell mass (ICM) derived cells as the cause of embryonic lethality. Loss of Mga leads to increased apoptosis in E4.5 embryos, though there is no decrease in the amount of cell proliferation. Embryos …
-
The Regulation of UHRF1 during Mammalian Embryogenesis
… potential: firstly during the development of the inner cell mass from the gametes, and secondly during the development of primordial germ cells. Although some of these changes in DNA methylation are correlated with gene expression, it is not yet understood why this epigenetic reprogramming is so …
-
Interleukin-6 Supplementation Improves Post-Transfer Embryonic and Early Fetal Development of in vitro Produced Bovine Embryos
… (IL6), an embryokine that increases inner cell mass (ICM) influences post-transfer embryonic survival and development of the embryo-proper and fetus. Four replicates of slaughterhouse-derived cumulus oocyte complexes underwent in vitro maturation and fertilization. On day 5 …
-
Investigating the consequences of chromosome abnormalities arising during pre-implantation development of the mouse
… of a mixture of diploid and aneuploid cells. Chromosome abnormalities are widely believed to contribute towards the relatively low success rates of IVF treatment. Consequently major efforts have been undertaken to develop effective tools to aid the selection of embryos with minimal …
-
Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
Cloning by somatic cell nuclear transfer (SCNT) forces cells to lose their lineage-specific epigenetic marks and become totipotent again. This reprogramming process often results in epigenetic and transcriptional aberrations that compromise development. Development rates after SCNT can thus serve …
-
A Novel Transgenic Rat Model for the Study of Germ Cell Biology
… a transgenic line of rats expressing GFP in all cells of the body, a serendipitous integration of a ROSA-EGFP transgene resulted in exclusive expression of EGFP in the germ cells of both sexes. EGFP expression was uniform and robust in cleavage stage embryos beginning at the late 2-cell stage and …
-
Cryo-electron microscopy studies of pluripotency factor Nanog
Embryonic stem (ES) cells are derived from the inner cell mass of the blastocyst in the early mammalian embryo. These cells are characterised by the ability to self-renew and to give rise to cell types from all lineages, known as pluripotency. Culture of ES cells in vitro requires addition of …
-
The role of polarisation in the first cell fate decision of the mouse embryo
… process in embryonic development is the first cell fate decision, when cells take on distinct lineage identities for the first time. In mammals, this separates embryonic inner cell mass (ICM) from extra-embryonic trophectoderm (TE) during pre- implantation development. In the mouse, the process …
-
Molecular control of embryonic stem cell identity
Embryonic Stem (ES) cells are the in vitro derivatives of the inner cell mass of a developing embryo, and exhibit the property of pluripotency, which is the ability of a cell to give rise to all cell lineages of an organism. Therefore, these cells hold great promise in the treatment of several …
-
Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… supplementation of IL6 increased blastocyst inner cell mass (ICM) cell number without affecting trophectoderm (TE) cell number. Additionally, we found that IL6 activated signal transducer and activator of transcription 3 (STAT3) specifically within ICM cells. LIF, however, did not affect ICM …
-
Cell lineage choice during differentiation of trophoblast stem cells (tsc) is dependent on oxygen levels, and mediated by stress enzyme pathways and mitochondrial function
… into three levels----integrate, organ/tissue or cellular response. Trophoblast tem cell (TSC) is one of the earliest differentiated stem cell within blastocyst, which is the stage of preimplantation embryo developed from fertilized egg. Different from another pluripotent embryonic stem cell, …
-
Determining the Signalling Pathways that Govern Human Naive Pluripotency
Conventional or “primed” human embryonic stem cells (hESCs) rely on FGF and TGFβ signalling for self-renewal, and occupy a developmentally advanced state of pluripotency comparable to mouse EpiSCs. Recent reports demonstrate that a naïve state of human pluripotency can be consistently derived …
-
In Vitro Models of Cellular Dedifferentiation for Regenerative Medicine
Stem cells have the ability to self-renew and to differentiate into a variety of cell types. Stem cells can be found naturally in the body, can be derived from the inner cell mass of blastocysts, or can be made by dedifferentiation of adult cells. Regenerative medicine aims to utilize the potential …
-
Investigating the Actions of Interleukin-6 Family Members on Post-Hatching Blastocyst Development in Cattle
… Adding IL6 at 25, 50, and 100 ng/ml increased inner cell mass (ICM) cell number but did not affect trophectoderm (TE) cell numbers. IL6 also increased the ICM:TE ratio before and after cryopreservation and ICM cell number after freezing. All IL6 doses reduced the number of cells undergoing …
-
Gene Expression in mESC Deficient in FGFR Signalling and the Identification of the Novel Gene Ened
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocyst stage embryos, and upon differentiation into embryoid bodies (EBs) they recapitulate the genetic, cellular and morphological events occurring in early embryogenesis. Among several signalling pathways and inductive …
-
Role of Histone Lysine Demethylase, KDM1B, in Trophoblast Stem Cell Self-Renewal and Differentiation
The first cell fate decision in development occurs at the blastocyst stage with the emergence of the trophectoderm (TE) and the inner cell mass (ICM). The TE is the precursor population of all major placental cell types. Reflecting this developmental plasticity, trophoblast stem cells (TSCs) can be …
-
Lineage tracing of normal human development and childhood cancers
From fertilisation onwards, the cells of the human body continuously experience damage to their genome, either from intrinsic causes or from exposure to mutagens. While the vast majority of DNA damage is repaired and the genome is replicated with extremely high fidelity, cells steadily acquire …
Page 1 of 2