Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"Inhibition studies"”.
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Structural, biochemical, and inhibition studies of proline biosynthetic enzymes
… at 1.80-2.35 A resolution. We also present inhibition data for the forward reaction of P5C reduction to proline in the presence of each analog.
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Diastereoselective acylation of trans-2-substituted-cyclohexanols and glycosidase inhibition studies
… Two compounds showed greater than 80% inhibition values in excess of the activity of β-D-glucosidases. More interestingly, the same compounds showed a marked enhancement of α-D-galactosidase activity by as much as 35%.</p>
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Kinetic, Mechanistic, and Inhibition Studies On the Deiminase Family of Enzymes
… utilize a reverse protonation mechanism. These studies have led to the development of the two most potent inactivators of AgD described to date, ABFA and ABCA. Both ABFA and ABCA are mechanism based inactivators. Studies to determine the mechanism of inactivation have been performed and the data …
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Kinetic Characterization of the Protein Arginine Deiminase Family: Mechanistic and Inhibition Studies
<p>The family of Protein Arginine Deiminases (PADs) include five mammalian isozymes (PAD1, 2, 3, 4, and 6) that catalyze the post-traslational modification of peptidyl-arginine to peptidyl-citrulline in numerous protein substrates. This modification results in numerous physiological and …
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Ribonucleotide reductase in dividing cells: Purification and inhibition studies with 4-hydroxynonenal
… HNE inhibited the CDP reductase activity. The inhibition was dependent on the concentration of HNE and the incubation time. The enzyme activity was reduced 500/0 by 0.1 roM HNE. The inhibitory effect of HNE was irreversible. DIT protected the enzyme against HNE suggesting that HNE inhibited the …
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Development of a cargo delivery system and inhibition studies focused on clostridium difficile toxin a
… inhibitor is described. Almost complete inhibition of toxin A was observed in cellulo by a peptide, identified to reversibly bind to the active site, HQSPWHHGGGC, that was functionalized with an epoxy group, HQSPGepoxyHHGGGC. Placement of the functional group is crucial for toxin …
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INHIBITION OF THE SERINE PROTEASE CHYMOTRYPSIN BY A SILANEDIOL PEPTIDE MIMIC: ASYMMETRIC SYNTHESIS AND INHIBITION STUDIES
… of amide hydrolysis and have shown outstanding inhibition of metallo and aspartic proteases. This research describes the synthesis and inhibition of the serine protease α- chymotrypsin by silanediol structures and the development of an efficient method to install the stereochemistry at the P1’ …
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Hapten inhibition studies assessing the ability of a thymine derived hapten to bind DNA antibodies in sera from patients with systemic lupus erythematosus (SLE)
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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LECTINS FROM THE HORSESHOE CRAB, LIMULUS POLYPHEMUS, REACTIVE WITH BACTERIAL LIPOPOLYSACCHARIDE
… R mutants of Salmonella minnesota. Precipitation-inhibition studies using 2-keto-3-deoxyoctonate (KDO) have supported the hypothesis that this acidic residue is the immunodeterminant group in the core region of the lipopolysaccharide molecule. Limulus also contains a lectin which interacts with …
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Unraveling the Dynamics of Ser126 Phosphorylation in Paxillin: A Focus on Phosphatase Activity
… we confirmed that PKC phosphorylates Ser126. Inhibition studies in endothelial cells revealed that calcineurin mediates Ser126 dephosphorylation. Phosphorylation at Ser126 enhances paxillin localization to focal adhesions, suggesting a dynamic regulatory mechanism in endothelial cell signaling.
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Field Amplified Sample Stacking on Amyloid Beta (1-42) Oligomers using Capillary Electrophoresis
… disease (1; 8; 10; 12-18). Additionally, recent studies have eluded to the impact of Alzheimer’s disease (AD) both economically and Socially in today’s society where an increase of about 71% of AD related deaths were recorded between 2000 and 2013 (7). Since the oligomeric forms of Aβ vary in …
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Glyphosate Resistance in Plant Cell Cultures
… uptake as a mechanism of glyphosate resistance. Inhibition studies of EPSPS by glyphosate however showed higher Ki values in three lines. One of these three lines (DHL 25), did not show consistent Ki values, but the observed values varied depending upon the growth stage of the cells at the time …
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Tetrachlorobiphenyl metabolism, toxicity, and regulation of cytochrome P50 expression in a marine teleost fish
… not seen with other hepatic enzymes. In vitro studies indicate that hepatic microsomal CYP1A1 is inactivated in the presence of TCB plus cofactor, likely due to the production of reactive oxygen species during TCB occupation of the active site. CYP1A1 inactivation by TCB in vitro may explain …
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Biochemical Evaluation of Lignin-like Molecules
… and size fractionated LMWLs. Detailed enzyme inhibition studies with sulfated and unsulfated LMWLs of ferulic and caffeic acid oligomers revealed that sulfation was not absolutely critical for dual inhibition property and smaller oligomers can yield a potent anticoagulant. Mechanistically, …
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Purification and characterization of malate dehydrogenase and 6- phosphogluconate dehydrogenase from Haemophilus influenzae
… 61,000. Initial velocity, product, and dead-end inhibition studies were consistent with an ordered bi-bi mechanism in which NAD is the first substrate bound to the enzyme. Several NAD analogs structurally altered in either the pyridine or purine moiety functioned as coenzymes in the reaction …
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Characterization of Homogeneous and Heterogeneous Anti-Nucleic Acid Autoantibodies in Systemic Lupus Erythematosus
… studied using different methods. Specificity studies characterizing monoclonal anti-ssDNA autoantibodies revealed specificity for nucleotides. Clone E-4-1 showed preferential binding with mono, di, and polyguanosine. Clones E-4-4 and E-11-1 specifically bound mono, di, and polythymidine. …
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Sodium phosphate inhibition of the growth of selected foodborne spoilage yeasts
Sodium phosphite was evaluated for inhibition of growth of spoilage yeasts in laboratory media and in two commercial carbonated beverages. In addition, the effects of pH and atmosphere in combination with sodium phosphite were also examined in laboratory media. Inhibition studies in laboratory …
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Dissecting the reaction mechanism of sheep liver 6-phosphogluconate dehydrogenase.
… 128A and H 186A and 16-fold for N 187A. Product inhibition studies by NADPH give a dissociation constant for the E:NADPH complex (Kis) that is increased by 5- to 6-fold for the S 128A and H 186A mutant enzymes at nonsaturating 6PG. No significant change is found in Kis value for the N 187A mutant …
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Hexose Transport In L6 Rat Myoblasts
… undifferentiated cells were used. Kinetic and inhibition studies suggested that the uptake of the glucose analogues 2-deoxyglucose and 3-O-methylglucose proceeds via two different transport systems, or at least two distinct forms of the same system.;Glucose transport mutants were isolated by …
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Synthesis of Novel Antimycobacterials and a Fluorescent Sensor for Simple Carbohydrates
… This compound should be useful in competitive inhibition studies with macrophages or as an immunological marker. We have successfully synthesized nonsulfated mimics of the aminosterol antibiotic from 5α-cholestan-3-one in two steps in 40-70% total yield. The critical step in this synthesis is …
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