Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Infectious clone"”.
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Investigating the role of non-structural genes in the genotype and phenotype of yellow fever vaccine strain 17D
… contribute to these differences. Using an infectious clone system, non-structural substitutions from 17D-204 virus were introduced into an Asibi infectious clone while Asibi virus residues were introduced into a 17D-204 infectious clone. The impact of the introduced substitutions on genetic …
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Genotypic and Molecular Determinants in the Continued Evolution of West Nile Virus in the United States
… and phenotypic properties of both natural and infectious clone-derived viruses. Phylogenetic analysis revealed bi-directional virus transmission (i.e., gene-flow) on the U.S.-Mexican border despite limited prior isolation or evidence of WNV circulation in northern Mexico; in addition, paired …
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Reverse Genetics-based Approaches to Attenuate Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
… PRRSV genes, we first established a DNA-launched infectious clone of the passage 14 PRRSV strain VR2385, pIR-VR2385-CA, and identified a spontaneous 435-bp deletion in the nsp2 gene. To characterize the biological and pathological significance of this nsp2 deletion, we restored deleted nsp2 …
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Contributions of Structural and Non-structural Genes to the Attenuation of the 17D Vaccine
… to the fitness and virulence of a virus. Infectious clone (IC)-derived chimeric viruses will be used to investigate contributions of NS protein genes to the generation of quasispecies population by assessing Shannon entropy of nucleotide diversity and frequency of RNA subpopulation in …
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Generation and Characterization of Flavivirus Receptor-Binding Domain Mutants and Chimeras
… were generated using the West Nile virus (WNV) infectious clone, into which EIIIs from a range of flaviviruses of increasing genetic diversity from WNV were substituted. Four versions of each chimera were generated using combinations of up- and downstream restriction sites. Of the constructs …
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Characterization of a recombinant chimeric yellow fever 17D vaccine - dengue-4 virus
… the impact of viral chimerization on a YFV-17D infectious clone where the 17D prME genes were replaced with those of the sylvatic wild-type DEN-4 strain P75-215, hereafter termed YFV 17D/DENV-4 prME chimera. Viral RNA of 17D vaccine, wild-type DENV-4, and YFV 17D/DENV-4 prME chimera were …
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Mutational analysis of the West Nile Virus NS4B protein
… utilized site-directed mutagenesis of a WNV NY99 infectious clone to introduce amino acid substitutions into the NS4B protein primarily targeting a highly conserved N-terminal domain, the variable central hydrophobic region, and the four cysteine residues. Out of fourteen recombinant viruses …
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Coronavirus Replicase Proteins: Multifunctional Mediators of Replication and Innate Immunity Evasion
… a temperature sensitive phenotype to MHV-A59 infectious clone virus. This virus (Alb ts6 icv) was significantly inhibited when incubated at the non-permissive temperature of 39.5°C. Processing of the p150 intermediate (nsp4-11) was unaffected; however, DMV formation was impaired. …
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Genetic characterisation and phylogenetic analysis of bovine astroviruses and kobuviruses
… one or multiple pathogens, as well as other non-infectious factors such as the environment and nutrition, contributes to its devastating effects. Astroviruses (AstVs) and Kobuviruses (KoVs) are two single-stranded, positive-sense RNA viruses previously detected in healthy and diarrhoeic calves. …
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Characterization of Avirulent Turkey Hemorrhagic Enteritis Virus: A Study of the Molecular Basis for Variation in Virulence and the Occurrence of Persistent Infection
… research should be the creation of a full-length infectious DNA clone, which could be used in the creation of a recombinant vaccine. The infectious clone would also allow for the systematic testing of genes that are suspected to be involved in virulence.
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Investigating the role of the West Nile virus and Koutango virus envelope protein in virulence phenotypes using structural protein chimeras
… into KOUV through the generation of an infectious clone (ic) enabled further study of the WNV/KOUV E proteins. Using the newly created KOUV ic, KOUV/WNV-EIII and -prME chimeras were produced and characterized. Unfortunately, the inclusion of an unexpected E protein mutation made …
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Host and viral determinants of flavivirus emergence and pathogenesis
… amino acid changes of these viruses into a ZIKV infectious clone, I define the contributions of these genetic changes to the enhanced virulence and transmission phenotype using in-vivo mouse and mosquito models. Here, I find that an NS4A substitution contributes to enhanced virulence and immune …
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Modulation of West Nile Virus Capsid Protein and Viral RNA Interaction through Phosphorylation
… by introducing the same mutations into a WNV infectious clone. Characterization of the mutant viruses showed that mutant viruses produced a lower viral yield and also experience a lag in viral production compared to wild type virus. Complementing mutant virus infected cells with wild type C …
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Characterization of grapevine vein clearing virus expression strategy and development of caulimovirus infectious clones
… of the GVCV large intergenic region were cloned and assessed for promoter activity, and the segment between nucleotides 7,332 and 7,672 was sufficient to drive expression of downstream ORF. 5' RACE and 3' RACE revealed that transcription was initiated predominantly at nucleotide 7,571 and …
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Usutu Virus: An Emerging Arbovirus Threat
… in the United States by: (1) developing infectious clones of recent European and African USUV isolates as tools for characterization and analysis of USUV and (2) assessing the transmission potential of several species of North American mosquitoes. In Aim 1, we show that the aforementioned …
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Adaptation of Chikungunya virus to Aedes albopictus mosquitoes: The role of mutations in the E1 AND E2 glycoproteins
… by Ae. albopictus, I developed full-length infectious clone (i.c.) (pCHIKV-LR i.c.) and an i.c. that expressed enhanced green fluorescent protein (eGFP) from either a 3’ or 5’ additional sub-genomic promoters (pCHIKV-LR 3’GFP and pCHIKV-LR 5’GFP respectively) based on a CHIKV strain isolated …
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Molecular Pathogenesis and Development of a Genetically Engineered Vaccine for Type-2 Porcine Circovirus
… a genetically-engineered vaccine, we constructed infectious DNA clones of both PCV1 and PCV2. By using the PCV2 infectious clone, we showed that pigs can be infected by direct intrahepatic injection of PCV2 infectious DNA clone. The pathological lesions and clinical disease associated with PCV2 …