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Showing 1 to 20 of 152 for “"Induced Pluripotent Stem Cells"”.
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Modeling Alzheimer's Disease in Induced Pluripotent Stem Cells
… that follows, we utilized recent advances in induced pluripotent stem cell (iPSC) and genome editing technologies to investigate two separate AD disease mechanisms: tau spreading and Aβ production in familial AD (FAD) PSEN1 mutants. Using TALENs, we generated a transgenic donor iPSC line that …
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Modelling Brugada Syndrome using induced pluripotent stem cells
… The ability to reprogram dermal fibroblasts to induced pluripotent stem (iPS) cells and to differentiate these into cardiomyocytes with the same genetic background provides a novel approach to studying inherited cardiac channelopathies with advantages over existing model systems. Whilst this …
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Correction of Cystic Fibrosis-Specific Induced Pluripotent Stem Cells
… the ability to genetically modify the resulting induced pluripotent stem cells (iPSCs) have provided an alternative to conventional gene therapies thus far attempted for CF. Here we describe the <em>in vitro</em> generation of iPSCs starting from fibroblast cells obtained from a CF patient. These …
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Derivation of lymphocytes from human induced pluripotent stem cells
Human pluripotent stem cells have the potential to produce essentially unlimited numbers of mature and functional blood lineage populations to study human hematopoiesis. Particularly, human induced pluripotent stem cells (hiPSCs) have the advantage to provide a source of autologous transplantable …
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Using human induced pluripotent stem cells to model cystinosis
… accumulation of cystine within lysosomes in most cells of the body, however the kidney is the main organ affected. For poorly understood reasons, renal proximal tubule (PT) cells lose their functionality and degenerate in cystinosis, forming a characteristic “swanneck” lesion that leads to kidney …
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Modeling Complex Neurological Disorders With Human Induced Pluripotent Stem Cells
… partly due to a lack of accurate models. Human-induced pluripotent stem cells (HiPSCs), which can be differentiated into any cell type and capture the genetic variation across humans, may serve as a powerful model to elucidate the mechanisms behind such disorders. In this thesis we have explored …
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Investigating Induced Pluripotent Stem Cells for Tissue Engineering and Hepatotoxicity Applications
Induced pluripotent stem cells (iPSCs) can be differentiated into multiple cell types in the body while maintaining proliferative capabilities. The generation of human iPSC-derived hepatocytes (iPSC-Heps) has resulted in a new source for hepatic cells. The current available options for human …
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Modeling Cancer Using Li-Fraumeni Syndrome Patient-Derived Induced Pluripotent Stem Cells
… by reprograming LFS patients' fibroblasts to induced pluripotent stem cells (iPSCs), and further differentiate these iPSCs into mesenchymal stem cells (MSCs) then to osteoblasts (OBs), the cells from which osteosarcomas originate. Interestingly, LFS iPSC-derived osteoblasts recapitulated the …
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DNA Repair Deficiency in Huntington's Disease Fibroblasts and Induced Pluripotent Stem Cells
… disease-causing TNR expansions occur in somatic cells remains elusive. Interestingly, HD has often been referred to a ‘DNA repair disease’, even though DNA repair dysfunction <em>in situ</em> has not been identified. We hypothesized that presence of the mhtt protein affects the expression of DNA …
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Development of collagen-based scaffolds for differentiation of induced pluripotent stem cells
… engineering. A collagen hydrogel can provide cells with a porous and soft environment to proliferate and differentiate. However, lacking mechanical stiffness and shrinkage resistance made it a challenge to sustain shape and size during a long stem cell differentiation process. In addition, a …
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MODELING HEART DISEASE OF PATIENTS WITH MUSCULAR DYSTROPHY USING INDUCED PLURIPOTENT STEM CELLS
… using DMD patient cardiomyocytes derived from induced pluripotent stem cells (iPSC). By converting adult somatic cells to iPSCs and further differentiate into cardiac lineage, a vast number of cardiomyocytes can be manufactured to explore disease etiology and conduct drug screening.
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MODELING FACIOSCAPULOHUMERAL MUSCULAR DYSTROPHY USING PRIMARY AND PATIENT-DERIVED INDUCED PLURIPOTENT STEM CELLS
… to the cell. Contractions lead to loss of repeat-induced silencing, allowing for transcription of DUX4. A common diagnostic method for FSHD shows demethylation of D4Z4 using gDNA of blood cells, and Kyba lab has shown that D4Z4 is demethylated in FSHD iPS cells, meaning that any cell type in an …
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Targeted mesenchymal stem cell differentiation from induced pluripotent stem cells for therapeutic application
<p>Over the past decade, mesenchymal stem cells have increasingly gained interest as a cell source for the treatment of a variety of diseases. This is because they play several roles in tissue homeostasis and regeneration, such as altering the immune response to limit inflammation, aiding tissue …
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INDUCED PLURIPOTENT STEM CELLS (IPSC) TO STUDY PATHOMECHANISMS ASSOCIATED TO AMYOTROPHIC LATERAL SCLEROSIS (ALS)
… recent possibility to obtain patient-derived induced pluripotent stem cells (iPSC) represents a great advancement to study neurodegenerative disorders, because iPSC maintain the individual genetic background and can be differentiated into different neuro- glial cell lineages, including …
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An improved method for generating oligodendrocyte progenitor cells from murine induced pluripotent stem cells
… to restore myelin in the central nervous system offer great hope for treatment of numerous conditions, ranging from multiple sclerosis and the leukodystrophies, to traumatic CNS injury. The oligodendrocyte progenitor cell (OPC) gives rise to functional oligodendrocytes following …
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Elucidating the underlying mechanisms of diabetic endotheliopathy using patient specific induced pluripotent stem cells
… The ability to generate functional endothelial cells (ECs) from induced pluripotent stem cells (iPSCs) is a novel and powerful tool for cell-based therapies. Human iPSC-derived ECs (iPS-ECs) have a broad range of clinical applications including cell-based therapy, disease modelling and drug …
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Using induced pluripotent stem cells to model glial-neuronal interactions in TDP-43 proteinopathies
… model these diseases, based on patient-derived induced pluripotent stem cells (iPSCs). This study presents the optimization of an iPSC-based platform to study the consequences of TDP-43 M337V mutation in human functional populations of MNs and astrocytes in isolation as well as in co-culture. To …
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Modeling of FUS- and C9ORF72-associated cortical neuropathology using patient-specific induced pluripotent stem cells
Amyotrophe Lateralsklerose (ALS) ist eine neurodegenerative Erkrankung, bei welcher speziell erste (kortikospinal) und zweite (spinal) Motorneurone (MN) von Neurodegeneration betroffen sind. Gegenwärtig bleibt ALS eine unheilbare Erkrankung. Der Tod tritt durchschnittlich 2 bis 5 Jahre nach …
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Modeling Alzheimer's Disease Using CRISPR/CAS9 Gene Editing and Induced Pluripotent Stem Cells Reveals Conserved Cellular Mechanisms
… A probable source of this lack of understanding stems from the poor disease model systems that have been utilized in the field for many years. Recently, advances in human induced pluripotent stem cell (iPSC) technology has enabled the study of uniquely human diseases, such as AD, in human tissue. …
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Modelling the early development of cartilage and bone in Mseleni Joint Disease using induced pluripotent stem cells
… non-Mseleni control, were reprogrammed into induced pluripotent cells (iPSC) by a collaborator. Pluripotency was confirmed by (qRT-PCR) gene expression analysis of the reprogramming genes OCT3/4, NANOG and SOX2 and immunocytochemical staining for their proteins. In vitro differentiation into …
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