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Showing 1 to 20 of 58 for “"Inclusion Bodies"”.

  1. Pathogenesis and Cytochemistry of Virus-Induced Nuclear Inclusion Bodies

    Reynolds, Harry Aaron, Jr.University of Illinois at Urbana-Champaign2014

    Made available in DSpace on 2014-12-09T22:30:13Z (GMT). No. of bitstreams: 1 6706712.pdf: 6775833 bytes, checksum: b14b6bb22ee8a047d80090ab813b0ae7 (MD5) Previous issue date: 1966

    uiuc Repository record

  2. Process-scale renaturation of recombinant proteins from inclusion bodies / by Nicholas Kotlarski.

    Kotlarski, Nicholas1998

    … Growth Factor-I analogue (LongR3IGF-I) as inclusion bodies within the bacterium Escherichia coli has been investigated. The principal focus was directed to the operation of refolding wherein the biological potency of the protein is imparted.

    adelaide Repository record

  3. Point mutational examination of eukaryotic initiation factor 4E (eIF4E), inclusion bodies analysis, and Barley Yellow Dwarf Virus (BYDV) exonuclease resistant RNA structure mapping and prediction

    Sheber, Melissa2018

    … (GST) tag repeatedly resulted GST-eIF4E inclusion bodies, which limited the ability to purify mutant eIF4E for binding studies. Therefore a large portion of this work is concerned with attempts to solubilize the GST-eIF4E fusion protein.</p> <p>Additionally, the second portion of this …

    iastate Repository record

  4. Identification of watermelon mosaic virus in pumpkin (Cucurbita pepo L.) in Virginia

    Butterfield, Fiona JaneVirginia Polytechnic Institute and State University1983

    … sections revealed the presence of cytoplasmic inclusion bodies, of pinwheel, scroll and tubular bundle formation, characteristic of WMV-1 infection, in addition to the amorphous inclusion bodies characteristic of WMV-2 infection. These cytoplasmic inclusion bodies were only found in the …

    vt Repository record

  5. Production and preliminary characterization of a fusion protein comprising streptavidin and a cellulose-binding domain

    Le, Duy KhaiUniversity of British Columbia1992

    … protein was overexpresed in E. coli and formed inclusion bodies. The soluble renatured protein recovered from the inclusion bodies bound both biotin and cellulose. It could be used to bind biotinylated proteins to cellulose.

    ubc Repository record

  6. Studies on the Cytoplasmic Inclusions Detected in Parkinson's disease

    류, 명이2011

    α-Synuclein-positive cytoplasmic inclusions are a pathological hallmark of several neurodegenerative disorders, including Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA). Synphilin-1, interaction partner of α-Synuclein is a major component of inclusion …

    ajou Repository record

  7. Role of Subcellular Differentiation in Plant Disease Resistance

    Lang, Saara SusannaVirginia Tech1997

    … of cytosol and accumulation of electron-dense inclusion bodies within the vacuoles. No structures resembling crystalloid ER or karmellae, caused by overexpression of hmgr in mammalian or yeast cells, respectively, were observed. Similar inclusion bodies were found in the vacuoles of wild-type …

    vt Repository record

  8. Isolation and characterization of bacterial polyhydroxybutyrate inclusions

    Kshetry, NinaMassachusetts Institute of Technology2006

    … accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHB within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using 13C …

    mit Repository record

  9. Identification of novel palmitoyl acyl transferases and characterization of the role of Huntingtin palmitoylation in Huntington Disease

    Huang, KunUniversity of British Columbia2008

    … which contributes to the formation of inclusion bodies and enhanced neuronal toxicity. By manipulating HIP14 levels through expression or knockdown, we can manipulate the number of huntingtin inclusion bodies and neuronal cell viability. Overall, these discoveries offer novel mechanism …

    ubc Repository record

  10. Membrane Systems in Cyanobacteria

    Liberton, Michelle

    … the identification and association of storage inclusion bodies with the membrane systems. This work addresses these outstanding questions via an analysis of two unicellular cyanobacteria, Synechocystis sp. PCC 6803 and Cyanothece sp. ATCC 51142. While the main focus of this work has been the …

    wustl Repository record

  11. Three cocluded insect viruses : a biophysical and biological study of the nuclear-polyhedrosis virus of Colias electo, the granulosis virus of Heliothis armigera and the nuclear-polyhedrosis virus of Heliothis zea

    Gitay, HelaDivision of Medical Microbiology1971

    … properties of the virus particles and their inclusion bodies were recorded, i.e. their resistance to chemical and physical treatments and their relative mobility in an electric field under standard conditions. Observations were made on procedures which brought about varying degrees of …

    cape-town Repository record

  12. Charakterisierung thermophiler Cellulasen aus Sulfolobus solfataricus und Thermotoga maritima

    Kufner, KristinaUniversität Bayreuth

    … wird SSO1949 rekombinant in unlöslicher Form („Inclusion Bodies“) überexprimiert. Die Aggregation des Proteins konnte auch durch Koexpression mit Chaperonen, Verkürzung der Ser/Thr-reichen Region sowie des C-Terminus, Deletieren des hydrophoben Loops innerhalb der konservierten Region und Fusion …

    bayreuth Repository record

  13. Determinants of Rotavirus Polymerase Localization and Activity

    McKell, Allison OverstreetVirginia Tech2017

    … nations. Within the infected host cell, RV forms inclusion bodies, called viroplasms, where many stages of viral replication occur. The RV polymerase, known as VP1, must localize to viroplasms during infection where it replicates the virus' RNA genome. The work described in this dissertation …

    vt Repository record

  14. A Systematic Method for Enhanced Expression of Alcohol Dehydrogenases in Escherichia coli

    Mital, SurajUniversity of Cambridge2022

    … achieved, challenges such as the formation of inclusion bodies, present limitations that inhibit streamlined manufacturing of recombinant enzymes. The lack of consistent workflows to address enzyme solubility issues often results in difficulties in producing high quantities of catalytically …

    cambridge Repository record

  15. Innovative Purification Protocol for Heparin Binding Proteins: Relevance in Biopharmaceutical and Biomedical Applications

    Batra, Sumit

    … of the D2 domain of FGFR from insoluble inclusion bodies, using a weak amberlite cation (IRC) exchanger. This approach is an alternative to conventional affinity column chromatography, which exhibit several disadvantages, including time-consuming experimental procedures and regeneration …

    wku-diss Repository record

  16. Virus-like particles as a vaccine against porcine reproductive and respiratory syndrome virus

    Venkatesh Murthy, Ambika MosaleVirginia Tech2013

    … to obtain hybrid HBcAg VLP protein from the inclusion bodies. This hybrid HBcAg VLP protein self assembled to 23nm VLPs that were shown to block virus infection of susceptible cells when tested on MARC 145 cells. Therefore, the safety of non-infectious and non-replicable VLPs and production …

    vt Repository record

  17. The Interplay of RSV and IFITs: A comprehensive analysis of human and bovine Interferon-Induced Proteins with Tetratricopeptide Repeats induction and interaction with human and bovine Respiratory Syncytial Virus

    Varga, MichalUniversity of Cambridge2024

    … RSV forms membrane-less perinuclear cytoplasmic inclusion bodies (IBs), recognised as sites for viral RNA transcription and replication. These IBs also manipulate cellular components, either repurposing them for viral benefit (e.g., components of the eIF4F complex) or inhibiting their function …

    cambridge Repository record

  18. Investigation of storage polysaccharide metabolism in lactic acid bacteria

    Kassem, Milad2012

    … für alle Zielproteine in Form von unlöslichen Inclusion-bodies. Es wurden verschiedene Versuche unternommen, um die Bildung von Inclusion-bodies zu verhindern und eine größere Menge an löslichem Protein zu erhalten. Verschiedene Ansätze, wie Expression bei niedrigen Temperaturen, Wachstum unter …

    goettingen Repository record

  19. Overexpression of Human Aryl Hydrocarbon Receptor in <i> E.coli</i> Using Two Different Solubility Enhancing Tags

    Tejomurthula, Sravanthi2018

    … using recombinant DNA technology. As AhR forms inclusion bodies due to its poor solubility in the cytoplasm of the host bacteria, it is tagged as a “difficult to express” protein. Therefore, it is challenging to generate functional recombinant AhR protein. My thesis documents the expression of …

    u-pacific Repository record

  20. Genetic and Biochemical Characterization of the Heteromeric Dihydroorotate Dehydrogenase From Bacillus Subtilis

    Kahler, Andrea EliseUniversity of Illinois at Urbana-Champaign2015

    … and purified. Overexpressed PyrDII formed inclusion bodies and could be purified by refolding and reconstitution with cofactors. Purified PyrDI was a flavoprotein, and refolded PyrDII bound 1 mol FAD and 1 mol [2Fe-2S] per mol subunit. The holoenzyme possessed dihydroorotate:NAD+ …

    uiuc Repository record

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