Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"In-vitro cytotoxicity"”.
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In vitro cytotoxicity and skin irritation testing of antimicrobial conjugated electrolytes : interactions with mammalian cells
An estimated 19,000 deaths and $3-4 billion in health care costs per year in the U.S. are attributed to methicillin-resistant Staphylococcus aureus (MRSA) infections. Certain cationic phenylene ethynylene (CPE)-based polymers (PPEs) and oligomers (OPEs) have been demonstrated to exhibit dark and …
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Knockdown of nfa1 Gene Cloned from Naegleria fowleri by Antisense RNA
PAME caused by N. fowleri is an acute, fulminant, and rapidly progressing fatal illness that usually affects children and young adults. There have been few reports to address proteins functioning in vitro cytotoxicity of N. fowleri. The nfa1 gene, cloned from a cDNA library of N. fowleri by …
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Cell-Mediated Cytotoxicity of Jensen Tumor in Sprague-Dawley Rats
<p>An in vitro system for the study of the cell-mediated immune response to Jensen Sarcoma (JS) is described. The tumor grew unrestricted and lead to the demise of 80% of successfully challenged Sprague-Dawley rats (persistors) while 20% of challenged rats (regressors) destroyed the tumor by an …
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Synthetic Studies in the Didemnin Series
Semi-synthetic studies directed toward didemnin analogues in which structural modifications were introduced in the side chain for investigating structure-activity relationships have been performed. The syntheses of several known didemnins including didemnin M, dehydrodidemnin B, and pyroglutamyl …
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AN INVESTIGATION OP POSSIBLE IN VITRO METHODS FOR STUDYING EYE IRRITANCY POTENTIAL
Two in vitro cytotoxicity procedures, namely the measurement of cell membrane integrity using fluorescein diacetate and ethidium bromide, and the quantitation of the release and activity of a cell membrane-bound enzyme, alkaline phospha1ase,were used to assess the qytotoxicity of a range of …
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Application of HCRSV protein cage for anticancer drug delivery
The icosahedral protein cage (PC) of the Hibiscus chlorotic ringspot virus (HCRSV) was explored as a platform for anticancer drug delivery. PC was prepared by removing viral RNA from coat proteins by dialysis against a Ca2+-deficient buffer of pH 8.0, and reassembling the coat proteins by dialysis …
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The development of group VIII trinuclear transition metal complexes as chemotherapeutic and photodynamic therapy agents
Cancer is a non-communicable disease that remains one of the leading causes of morbidity and mortality globally. The detrimental effects of cancer are exacerbated by the emergence and development of resistance to current treatment modalities. The development of new treatment modalities, along with …
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Expanding the versatility of aminoquinoline organometallic complexes as anticancer and antibacterial agents
Cancer is a devastating non-communicable disease which continues to increase its burden on countries globally. Despite the numerous research and clinical breakthroughs, its prevention is one of the most significant health challenges of the 21st century. Chemotherapy is amongst the most common and …
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Synthesis and biological evaluation of auristatin-F recombinant antibody-drug conjugates for cancer therapy
This study serves as a contribution to the emerging field of antibody-drug conjugate (ADC) therapeutics, in which novel ADCs were generated and biologically evaluated for therapeutic potential and specificity towards cancer cells. SNAP-tag site-directed conjugation was employed in order to overcome …
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Biologische und physikochemische Charakterisierung sowie 3D-Wachstum von Zellen auf Matrices aus nativem Kollagen für den Einsatz in der Medizin
Collagen sponges are used in medicine because of their good biological and physicochemical properties and suitability for drug delivery, reconstructive skin surgery, and replacing lost tissue in subjects with burn injuries. A number of collagen matrices are currently in clinical use and available …
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Synthesis, Characterisation and Biological Activity of Silicon Nanoparticles Functionalised by Anticancer Compounds
Abstract Silicon Nanoparticles (SiNPs) are non-toxic materials particularly suitable for various biomedical applications. They exhibit unique optical properties for use in bioimaging and diagnostic applications. To exploit these aspects, this thesis explores the synthesis, characterisation and …
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Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity
In our search for new anti-tuberculosis lead molecules, five medicinal plant species, Olea capensis (L.l, Tulbaghia alliacea (L.), Inula graveolens (L.), Leyssera gnaphaloides (L.), and Buddleja saligna (L.) were collected in Cape Town and surrounding area and investigated for antimycobacterial …
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The suitability of biodegradable poly(dl-lactide-co-glycolide)75:25 microspheres for the sustained release of antibiotics
Post-operative infections resulting from total hip arthroplasty are caused by bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa entering the wound perioperatively or by haemetogenous spread from distant loci of infection. They can endanger patient health and require expensive …
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Synthesis, characterization, and anticancer evaluation of novel gold(I) phosphine thiolate complexes : solubility, cellular uptake, and cytotoxicity.
… and evaluation of novel gold(I) phosphine thiolate complexes as potential anticancer agents. Inspired by the activity of auranofin and the limitations of existing platinum-based drugs such as cisplatin, this research sought to explore the therapeutic potential of systematically varied …
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Synthesis, Properties, and Biology of Advanced H2S-Releasing Materials
… sulfide (H2S) is an endogenously produced signaling gas involved in numerous cellular functions. At the appropriate concentration, exogenous administration of this gasotransmitter regulates vasodilation, promotes angiogenesis of endothelial cells, and generally exhibits beneficial effects as an …
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A three-dimensional agent-based model of glioblastoma with applications towards in vitro and in vivo therapy simulation
Glioblastoma is an aggressive and highly infiltrative malignant brain tumor with a poor survival rate. Treatment options for patients are limited and have remained stagnant for almost two decades despite dozens of clinical trials. Advancements in genomic characterization methods allowed for the …
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Application of pharmaceutical technologies to improve the activity of cancer chemotherapeutic agents
Cancer is a leading cause of death worldwide. Chemotherapy remains a major therapeutic modality in cancer treatment. Conventional chemotherapeutic agents usually have limited efficacy. Furthermore, chemotherapeutic agent- and/or excipient-related toxicities comprise a serious problem that may, in …
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Nucleic acid clamp-mediated transcriptional and translational modulation of oncogenes via the stabilization of G-quadruplexes
… secondary nucleic acid structures that exit in various biologically important regions within a cell, such as the promoters of DNA, 5’-Untranslated regions (UTR) of mRNA and telomeres. These secondary structures tend to cluster around 1 kb from transcriptional start sites across all human …
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G-Quadruplexes As Molecular Targets For Transcriptional Control
DNA G-quadruplexes (G4s) have gained interest in modern drug discovery efforts. The majority of G4 research, including the mass of this research, heavily focuses on cancer therapeutic development, as G4s are more pronounced in oncogenic promoters compared to the rest of the genome, our efforts …
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