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Showing 1 to 20 of 34 for “"In vitro translation"”.
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The in Vitro Translation of Satellite Tobacco Necrosis Virus Ribonucleic Acid
Made available in DSpace on 2014-12-10T23:24:30Z (GMT). No. of bitstreams: 1 7405610.pdf: 4293299 bytes, checksum: f4f05f2afd28024a6feabb1e507e732a (MD5) Previous issue date: 1973
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Regulation of Glucanohydrolases in Nicotiana Tabacum on the Messenger-Rnalevel (beta-1,3-Glucanase, Chitinase, Cytokinin, Auxin, Complementary-Dna Clone)
… abundant enzymes (beta)-1,3-glucanase and chitinase in cultured tobacco (Nicotiana tabacum L. cv. Havana 425) tissues is inhibited by the hormones auxin and cytokinin. As judged by immunosorption and partial proteolysis of the in vitro translation products, poly(A)+ RNA from tobacco tissues …
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The Ribosome Discriminates the Structure of the Amino Acid at its Peptidyl-Transferase Center
Contemporary interpretations of Crick's original Adaptor Hypothesis view both the amino acid and the tRNA body as passive participants during aminoacyl-tRNA (aa-tRNA) selection by the ribosome. Recent experimental evidence investigating tRNA mutants that miscode due to mutations that lie outside …
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Transcriptional and Translational Products of Avian Erythroblastosis and Avian Myeloblastosis Viruses
<p>The transcription and translation products of two avian acute leukemia viruses, avian erythroblastosisvirus (AEV) and avian myeloblastosisvirus (AMV), were examined. AEV is defective in virus replication and requires the presence of a helper virus to produce infectious progeny. AEV-transformed …
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A Pseudoknot Improves Selection Efficiency in Ribosome Display
… between the ribosome, mRNA and translated protein. To investigate if mRNA secondary structure improves stability of the complex, I tested a pseudoknot, originating from the genomic RNA of infectious bronchitis virus (IBV), a member of the positive-stranded coronavirus group. I used the …
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The production, design and application of antimicrobial peptides
With the spread of drug-resistant bacteria, existing antibiotics are losing their potency. Antimicrobial peptides (AmPs) represent an exciting class of drug candidates, particularly because their mechanism of action is unlikely to induce drug resistance. If resistance to AmPs were also slower to …
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Evidence for post-transcriptional regulation of induction of NADP- specific glutamate dehydrogenase by accumulation of its mRNA in uninduced synchronous Chlorella cells
The mRNA coding for the ammonium inducible NADP-specific glutamate dehydrogenase (NADP-GDH) from Chlorella was studied in induced and uninduced cells to determine the molecular mechanisms which regulate the cellular levels of this enzyme. A procedure for isolation of a high yield of total …
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Characterization of 5 UTR splicing and the CGI in HIV-2 RNA
… cycle via regulatory elements found within the 5 untranslated region of HIV-2 genomic RNA. Two elements of interest are the 5 UTR intron and the proposed long distance base pairing interaction between the C-box and G-box termed the CGI. This research focuses on the effects of 5 UTR …
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Characterization of 5 UTR splicing and the CGI in HIV-2 RNA
… cycle via regulatory elements found within the 5 untranslated region of HIV-2 genomic RNA. Two elements of interest are the 5 UTR intron and the proposed long distance base pairing interaction between the C-box and G-box termed the CGI. This research focuses on the effects of 5 UTR …
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The relationship between two forms of glycogen phosphorylase in Dictyostelium discoideum
… system to study eukaryotic cell differentiation. In D. discoideum glycogen degradation provides precursors for the synthesis of developmentally regulated structural end products. The enzyme responsible for glycogen degradation, glycogen phosphorylase, exists in active and inactive forms. The …
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Site Directed Mutagenesis of a Putative Protease Cleavage Site Within the 105 Kilodation Protein of Southern Bean Mosaic Virus
There are four open reading frames (ORF) in three positive-sense translational phases of Southern Bean Mosaic Virus (SBMV). ORF2 codes for a 105 KDa polyprotein which is proposed to be autocatalytically cleaved into smaller functional proteins. Amino acid sequence homology between the SBMV and …
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Initiation of Translation of Eukaryotic Messenger-Rna at a Unique Site Lacking an AUG Codon
The initiation of translation of eukaryotic mRNA is believed to proceed by a "scanning mechanism" where the ribosome recognizes a 5$\sp\prime$ terminal cap structure (m$\sp7$GpppN ...) and then scans down the mRNA until reaching the first 5$\sp\prime$ terminal AUG or $\sp{\rm A}\sb{\rm …
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Genome Organization And Expression Of Selected Potexviruses
We have used in vitro translation to analyze the protein products produced from the genomic RNAs of members of the potexvirus group, namely, papaya mosaic virus (PMV), clover yellow mosaic virus (CYMV), viola mottle virus (VMV), barrel cactus virus (BCV), and foxtail mosaic virus (FTMV). …
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Characterization of KPAP, a Novel Pokeweed Antiviral Protein Isoform
… plant that harbors the pokeweed antiviral protein (PAP) genes which belong to the N-glycosidase family. These proteins are called ribosome inactivating proteins (RIPs) which remove an adenine base from the sarcin/ricin loop (SRL) of the 28S rRNA in eukaryotes. This depurination results in …
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Identification and molecular characterisation of a putative gene for cell detaching factor from Trichomonas vaginalis.
Trichomonas vaginalis (Tv) is the etiologic agent of trichomoniasis, a sexually transmitted disease of significant socio-economic importance. Cell-free filtrate from Tv growth media causes rounding and detachment of adherent monolayers of human epithelial cells in culture. Cell detaching factor …
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Characterization of Dna Complementary to Rabbit Cytochrome P-450 Messenger-Rnas and Studies on Their Differential Regulation by Phenobarbital (Gene, Expression)
In order to understand the regulated biosynthesis of cytochrome P-450 I have prepared a clone bank of mRNA extracts from the liver of a rabbit treated with phenobarbital. Plasmid cDNAs were selected as potential P-450 clones by plus-minus hybridization, in vitro translation of hybrid-selected mRNA, …
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Ribosomal Synthesis of N-methylated peptides
… isolated from various organisms often contain N-methylated backbones. Such a modification of backbone of the peptide changes its conformational rigidity. This modification improves the biological properties of the peptide, such as improved target affinity, proteolytic stability or membrane …
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Macrolide Resistance in Mycobacterium avium
… avium isolates resistant to clarithromycin and azithromycin have been recovered from patients undergoing antibiotic therapy. Comparison of DNA fingerprints of sensitive and resistant isolates showed that resistance resulted from mutation of the original, sensitive isolate in five of seven …
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Evaluation of translation initiation features of satellite tobacco necrosis virus RNA
… an unusual mRNA that is efficiently translated in the absence of a 5$\sp\prime$-terminal cap structure and initiator AUG codon. The translational properties, in wheat germ in vitro translation system, of transcript STNV RNAs (tSTNV RNAs), with modifications to the initiator AUG codon and the …
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Molecular Mechanisms of SURF4- mediated Protein Secretion
Protein secretion is an essential process that drives organelle biogenesis, cell growth and communication. Once synthesised and processed in the Endoplasmic Reticulum (ER), secretory proteins are incorporated into transport carriers that are generated by the COPII coat. Efficient and accurate cargo …
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