Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"In vitro maturation"”.
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Factors influencing in vitro maturation of pig oocytes
The present studies were undertaken to investigate the effect of different medium supplements, the involvement of steroid hormones and the role of estradiol-17$\beta$ on in vitro maturation of pig oocytes. Cumulus oocyte complexes (COC) were obtained by aspiration of 2-5 mm follicles. The oocytes …
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Strategies to improve in vitro maturation of human and mouse oocytes
Our knowledge of reproductive medicine has expanded rapidly since the birth of Louise Brown, the first baby to be conceived by in vitro fertilization (IVF) in 1978. In vitro maturation (IVM) of oocytes - a culture technique to support maturation of immature oocytes in vitro - could offer an …
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In vitro maturation and fertilization of porcine oocytes and evaluation of male fertility
The objectives of this thesis were to establish incubation systems for in vitro porcine maturation and for in vitro fertilization (IVF), and utilizing these in vitro matured oocytes and in vitro fertilization techniques to determine the difference of sperm fertility from different boars. In the …
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In Vitro Maturation, Fertilization and Embryo Culture of Porcine Gametes in a Microfluidic Environment
These experiments were designed to examine the effects of in vitro fertilization within the microchannel environment and to compare the results to the traditional microdrop culture system. Additionally, the initial experiments of integrating the steps of the IVP process within a single microchannel …
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Effects of In Vitro Maturation and Fertilization of Cattle Ova on Rate of Twinning in Beef Cattle
… Texas State University, San Marcos, to examine the effects of using in vitro matured and fertilized embryos in embryo transfer to induce twinning and examine the influence of twinning on cow production and carcass characteristics between single and twin calves. Fifteen single parity cows …
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Exogenous γ-Glutamyl Cycle Compound Supplementation to In Vitro Maturation Medium and the Effects on Subsequent In Vitro Fertilization and Culture Parameters of Porcine Oocytes and Their Impact on Embryo Viability
High concentrations of intracellular glutathione enhance the in vitro production of porcine embryos. Six experiments were conducted to study the effects of varying concentrations of different supplements to the in vitro maturation (IVM) medium on in vitro fertilization (IVF) and in vitro culture …
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IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae)
The studies presented in this thesis were designed to develop efficient protocols for collecting competent oocytes and to produce disease-free wood bison embryos by in vitro fertilization and culture. In Chapter 3, the in vivo and in vitro maturational characteristics of cumulus-oocyte complexes …
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Genomic studies of oocyte physiology reveal mechanisms critical to oocyte competence
In vitro maturation of porcine oocytes has been intensively studied for many years. However, the in vitro maturation system is still inefficient to produce competent oocytes that can develop to viable embryos. The studies discussed in this dissertation attempt to use different techniques and …
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Analysis of Oocyte Quality in the Rhesus Macaque (Macaca mulatta)
Many primate populations face the threat of extinction due to habitat loss, intensive agriculture, hunting for meat, the pet trade and/or use in traditional medicines. An alternative approach to in situ conservation includes gene banking and the use of assisted reproductive technologies (ART), such …
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Involvement of mitochondrial transcription factor A (TFAM) in porcine gametogenesis and preimplantation embryo development
… Factor A (TFAM) is responsible for maintenance and transcriptional control of mitochondrial DNA (mtDNA). TFAM knockout is embryolethal. We have studied the expression and distribution of TFAM in the gametes and preimplantation embryos of a domestic pig (Sus scrofa), a livestock species …
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Mitochondrial DNA replication in pre-implantation embryonic development
… mitochondrial DNA (mtDNA), which is maternally inherited through the oocyte, its replication being regulated by nuclear-encoded replication factors. It was hypothesised that mtDNA replication is highly regulated in oocytes, pre-implantation embryos and embryonic stem cells (ESCs) and that this …
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Oncofertility: καρκίνος σε παιδιά, εφήβους και νεαρές γυναίκες και διατήρηση της γονιμότητας
… και πιο εξειδικευμένες προσεγγίσεις, όπως η in vitro maturation (IVM) και η μετάθεση ωοθηκών. Επιπλέον, διερευνώνται οι ηθικές, ψυχολογικές και νομικές παράμετροι της ογκογονιμότητας, τόσο σε διεθνές επίπεδο όσο και στο ελληνικό σύστημα υγείας. Σκοπός της εργασίας είναι να αναδείξει τη …
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Interleukin-6 Supplementation Improves Post-Transfer Embryonic and Early Fetal Development of in vitro Produced Bovine Embryos
In vitro produced (IVP) bovine embryos are useful for investigating the mechanisms affecting early embryonic failure. The work in this thesis explored how interleukin-6 (IL6), an embryokine that increases inner cell mass (ICM) influences post-transfer embryonic survival and development of the …
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In-vitro developmental potential of bovine oocytes obtained by transvaginal follicular aspiration as related to their morphological quality and after microinjection of DNA
The development of oocytes of differing quality retrieved using transvaginal follicular aspiration (TVFA) and following DNA injection was examined. Eight cows were subjected to twice weekly TVF A for 16 wk. Oocytes retrieved were graded and placed in an in-vitro maturation, fertilization and …
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Developing embryo technologies for the eland antelope (Taurotragus oryx)
Assisted reproductive technologies developed in domestic cattle serve as a starting point in similar studies on nondomestic bovids. The common eland is a useful model species for studies on rare tragelaphine antelopes. In Chapter 3 of the present study, effects of components/attributes of …
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Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
Three experiments were conducted to determine an efficient method of obtaining oocytes from cows via ultrasound-guided transvaginal follicular aspiration. Transvaginal oocyte recovery combined with in vitro maturation, fertilization, and culture (IVMlIVFIIVC) can produce pre-implantation stage …
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Detecção viral e capacidade de maturação in vitro de ovócitos de vacas naturalmente infectadas pelo Herpesvirus bovino 1
… verificar a presença do DNA do herpesvirus bovino 1 (BoHV1) em Complexos Cumulus Oóforus (COCs) e no sangue, além de avaliar a capacidade de desenvolvimento de ovócitos oriundos de vacas infectadas naturalmente. Os COCs foram obtidos de 15 doadoras por meio de aspiração folicular guiada por …
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Biochemical and spectroscopic insights into peculiar active site structures and biosynthesis of O2-tolerant [NiFe]-hydrogenases
[NiFe]-hydrogenases are metalloenzymes catalyzing the reversible cleavage of H2 into two H+ and two e–. They are particularly interesting for clean and sustainable H2-based energy conversion approaches or the H2-driven regeneration of nucleotide cofactors. Their active site consists of a …
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Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence.
Paracrine factors secreted by the oocyte (oocyte-secreted factors, OSFs) regulate a broad range of cumulus cell functions. The capacity of oocytes to regulate their own microenvironment by OSFs may in turn contribute to oocyte developmental competence. The aim of this thesis was to examine whether …
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