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Showing 1 to 12 of 12 for “"In vitro expression"”.
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In vitro Expression of Human Zona Pellucida Protein 3
<p>Human zona pellucida protein 3 (hZP3) is the putative receptor on the zona pellucida of the mature oocyte that recognizes and binds sperm, and therefore plays a critical role in fertilization.</p> <p>A full length cDNA of hZP3 (1278 bp) was amplified from the human ovary mRNA by reverse …
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Molecular Cloning, In Vitro Expression, and Functional Characterization of an Ovine Gastrointestinal Peptide Transporter (oPepT1)
… the primary structure, tissue distribution, and in vitro functional characterization of a peptide transporter, oPepT1, from ovine intestine. The ovine intestinal oPepT1 cDNA was 2,829 bp long encoding a protein of 707 amino acid residues with an estimated molecular size of 79 kDa, and a pI of …
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Towards ultrahigh-throughput phenotypic screening using microfluidic droplets and functionalised polyacrylamide beads
… have been harnessed by human society to advance industry and medicine. However, evolution is primarily selective for survival of the organism and/or gene rather than any particular activity directly, and is largely constrained by its cellular environment — interference or negative interactions …
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Molecular and cellular expression of novel genes associated with breast cancer
An improved and individualised breast cancer care can be pursued with the use of tumour markers that are associated with risk assessment, diagnosis, prognosis, prediction of treatment outcome, monitoring of the disease and development of novel therapies. As such, this study proposed to characterise …
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Construction of a COL11A1 Transgene Vector
… Cartilage disorders affect millions of people in the United States alone, with effects ranging from poor skeletal development and joint pain to shortened lifespan and perinatal lethality. Many of these disorders have their root in defects of collagen, type XI collagen being among the most …
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Analysis of Aurora B Regulation and Signaling
Aurora B is a serine/threonine kinase that functions in a complex with two other chromosomal passenger proteins called INCENP and Survivin. Its function is implicated in a variety of processes related to mitosis, such as chromosome condensation, regulation of arm cohesion, spindle assembly, …
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Characterization and Functional Analysis of a Newly Identified Human MT5-MMP Transcript Variant Isolated from Multipotent NT2 Cells
Membrane-type 5 matrix metalloproteinase (MT5-MMP) is unique among MMP family members as it is predominately expressed in the CNS. Its expression is ubiquitous during brain development and restricted to regions of neurogenesis and neuroplasticity in the adult. MT5-MMP is a mediator of pericellular …
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Die Rolle der Serin/Threonin Kinase Cot in Proliferation, Differenzierung und Apoptose
… zum Zellkern über komplexe z.T. miteinander verknüpfte Signaltransduktionskaskaden übertragen. In der vorliegenden Arbeit wurde das Proto-Onkoprotein Cot als eine neue Komponente von mitogenen Signalkaskaden identifiziert, die die Fähigkeit hat, sowohl die klassische mitogene Kaskade …
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An evaluation of cancer biomarkers in normal ovarian epithelial cells and ovarian cancer cell lines
Introduction: Globally, there are over 190,000 new reported cases of ovarian cancers per annum. This comprises 3% to 4% of all cancers in women. Ovarian cancer is one of the leading causes of deaths in women. Ovarian cancer is the second most diagnosed gynaecological malignancy and over all the …
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IL-2 and Antisense TGF-B1 Gene Therapy of the H238 Tumor and Analysis of TGF-B Receptors
<p>Transforming growth factor-beta (TGF-p) belongs to a family of immunosuppressive cytokines, capable of regulating macrophages, T, B and NK cells. We have detected the TGF-p transcript being synthesized by H238 cells. This is herpes simplex-Type2 transformed murine fibrosarcoma cell line. The …
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The role of citrullination of central nervous system proteins in multiple sclerosis.
Multiple sclerosis (MS) is a chronic inflammatory neurodegenerative disease of the central nervous system (CNS) characterised by focal lesions of inflammation and demyelination, with subsequent axonal damage. Although the cause is not yet known, it is thought to be autoimmune in origin. Antibodies …
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PATHOPHYSIOLOGY OF VON WILLEBRAND FACTOR IN BLEEDING AND THROMBOSIS
… factor (VWF) is a multimeric glycoprotein mainly known to be involved in primary hemostasis recruiting platelets at the site of damaged vessels and acting as factor VIII (FVIII) carrier. Quantitative or qualitative alteration of VWF protein is responsible for von Willebrand disease …