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Showing 1 to 9 of 9 for “"In vitro embryo production"”.
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IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae)
The studies presented in this thesis were designed to develop efficient protocols for collecting competent oocytes and to produce disease-free wood bison embryos by in vitro fertilization and culture. In Chapter 3, the in vivo and in vitro maturational characteristics of cumulus-oocyte complexes …
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Modulation of bovine preimplantation embryonic development and pregnancy by FGF2, LIF, and IGF1
In vitro embryo production enhances genetic selection through capitalizing on superior genetics, improving fertility, and increasing animal efficiency, all of which contribute to sustainable food animal production. Of the livestock species, cattle use more embryo technologies than any other. While …
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Transcriptional profiles of cumulus-oocyte complexes related to developmental competence in bovine oocytes
During folliculogenesis, oocytes and cumulus cells undergo many morphological and physiological changes. Transcriptome data were produced from single oocytes and corresponding cumulus cells (CC) to infer the differences in the transcript abundance from fully grown versus growing phase oocytes and …
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Interleukin-6 and its Contribution to Embryogenesis in Cattle
In vitro systems like those used for in vitro embryo production are invaluable for our understanding of embryogenesis and the processes that regulate it. However, extensive research has also highlighted that in vitro produced embryos negatively differ from their in vivo counterparts in various …
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Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
Three experiments were conducted to determine an efficient method of obtaining oocytes from cows via ultrasound-guided transvaginal follicular aspiration. Transvaginal oocyte recovery combined with in vitro maturation, fertilization, and culture (IVMlIVFIIVC) can produce pre-implantation stage …
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Post-Transfer Outcomes in Cultured Bovine Embryos Supplemented with Epidermal Growth Factor, Fibroblast Growth Factor 2, and Insulin-Like Growth Factor 1
The high incidence of pregnancy loss is a major issue facing the cattle industry. Use of in vitro fertilized (IVF) bovine embryos has become increasingly popular to help alleviate several of these reproductive issues and provide a means to enhance genetic gain for production traits. An uterine …
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A Genomic Perspective on Early Embryo Development in Cattle
In vitro embryo production (IVP) has revolutionized the cattle industry, but it still faces efficiency limitations compared to in vivo embryo production. With a major impact on it, a proper transcriptome profile is crucial for an embryo to undergo pre-implantation development and become …
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Factors affecting the quality and function of the bovine periovulatory follicle
… management programs. Opportunities for improving fertility exist within the numerous challenges related to reproductive management. Non-conventional, creative tools for reproductive management could help producers overcome these challenges. In an effort to produce information that could be …