Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"In silico Predictions"”.
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Data-driven predictive modeling for cell line selection in biopharmaceutical production
… development cycle is the selection of the cell line that will become the Master Cell Bank for product manufacturing for clinical and commercial use. This cell line selection process is resource-intensive, requiring several months, involving hundreds of cell cultures and corresponding assays, and …
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Principled Methods and Models for Deep Learning Based Functional Genomics
Many advances in functional genomics and in biology more broadly can be attributed to the rise of massively parallel sequencing technology and its derivatives. As the volume of sequencing and other high-throughput experimental data increases exponentially, so does the need for computational methods …
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Evaluation of Select Publicly Available in Silico Methods for Predicting Functional Effects of Missense Mutations in the GALNS Gene
… to sequence patient DNA has led to an explosion in the reports of mutations for a number of diseases. Frequently, published reports include in silico predictions of the probability that the mutations are disease-associated. The question asked here is how well these in silico methods predict the …
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Protein-dependent prediction of messenger RNA binding using Support Vector Machines
RNA-binding proteins interact specifically with RNA strands to regulate important cellular processes. Knowing the binding partners of a protein is a crucial issue in biology and it is essential to understand the protein function and its involvement in diseases. The identification of the …
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Characterisation of the localisation and function of the Bacillus subtilis YuaB protein during biofilm formation
Bacteria can actively communicate and interact with each other to establish multicellular communities. Many of these processes involve functional differentiation of cells into specialised subpopulations by expression of varying genetic programmes. This leads to division of labour between the …
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Evolutionary and Paleobiochemical Analyses of Heme Peroxidases
… of enzymes with desirable characteristics for industry. The approach presented here, applied to the heme peroxidases, marries bioinformatic methods with evolutionary theory and biochemical validations. Heme peroxidases catalyse the oxidation of a variety of electron donors by hydrogen peroxide. …
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Structure-based Predictions for Molecular Initiating Events
Toxicity testing of chemicals is currently undergoing its largest ever paradigm shift, moving towards faster, cheaper and more human-relevant methods which focus on mechanistic understanding. An AOP provides a framework for organising biological knowledge and data. The gateway to an AOP is the MIE, …
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Probing the role of histone acetylation in the evolution of pathogenicity in <i>Candida glabrata</i>
With the advancement of modern medicine, invasive fungal infections are a growing cause of global mortality.<i> Candida glabrata</i> accounts for 25% of fungal infections in UK hospitals, second only to<i> C. albicans</i>. This number is expected to rise given current treatment challenges due to …
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Exploring the effects of polymorphic variation on the stability and function of human cytochrome P450 enzymes in silico and in vitro
… drugs. Polymorphic variation can result in altered drug efficacy as well as adverse drug reactions so the lack of understanding of the effects of single amino acid substitutions on cytochrome P450 drug metabolism is a major problem for drug development. In order to begin to address this …
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In silico identification of genes regulated by abscisic acid in Arabidopsis thaliana (L.) Heynh.
… plant hormone that plays an important role during plant growth and development. During vegetative growth ABA mediates (in part) responses to various environmental stresses such as cold, drought and high salinity. The response triggered by ABA includes changes in the transcript level of genes …
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Computational pharmaceutics approaches to inform drug developability: focus on lipid-based formulations
Purpose: Declining productivity in the face of increasing numbers of poorly water-soluble drugs has fast-tracked necessity for predictive tools which assess the delivery potential of bio-enabling formulations. However, there is a perceived risk associated with early-stage selection of bio-enabling …
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Functional identification of molecular oncotargets associated with the resistance to ALK inhibition in neuroblastoma via genome-wide CRISPR-Cas9 screens
Recent whole-exome sequencing studies of hundreds of high-risk neuroblastoma (hNB) patients have identified Anaplastic Lymphoma Kinase (ALK) as the only directly ‘druggable’ target with a significant mutation rate (9%). ALK is a receptor tyrosine kinase whose dysregulation has been implicated as …
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On the aberrant nature of tau protein: biophysical approaches to characterise and target aggregation in neurodegeneration
Tau is a multifaceted and dynamic protein vital to a number of physiological processes, notably in the architecture of the cytoskeleton. Its key role in the assembly and stabilisation of microtubules, which are critical for molecular shuttling and axonal growth, underscores the significance of tau …
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EXPANSION OF CLINICAL PHENOTYPE AND USE OF KETOGENIC DIET IN RARE GENETIC CONDITIONS: GLUCOSE TRANSPORTER TYPE 1 DEFICIENCY SYNDROME AND SCN8A-RELATED DISORDERS
… between 264 and 446 million people worldwide and include a broad group of genetic conditions that manifest with epilepsy and neuropsychiatric disorders with paediatric onset. This thesis focused on the study of two rare genetic disorders: Glucose Transporter Type 1 Deficiency Syndrome (GLUT1-DS) …