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Showing 1 to 18 of 18 for “"Immunotoxin"”.
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Construction of a recombinant immunotoxin
… recent years a number of therapeutically useful immunotoxins have been produced using recombinant gene technology. In general, this involves fusion of a toxin gene with sequence encoding a variety of clinically relevant proteins or peptides. Using these techniques a recombinant immunotoxin has …
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Immunotoxin Monotherapy and Combinatorial Therapy With Immune Checkpoint Inhibitors for Malignant Brain Tumors
… this need, we have developed two novel targeted immunotoxins (ITs), D2C7-(scdsFv)-PE38KDEL (D2C7-IT) and NZ-1-(scdsFv)-PE38KDEL (NZ-1-IT). D2C7-IT was developed by fusing the single-chain variable fragment (scFv) of the D2C7 monoclonal antibody (mAb) with domains II and III of Pseudomonas …
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Herstellung und Charakterisierung von rekombinanten, ETA-basierten CD64-Immuntoxinen zur Bekämpfung von dysregulierten Makrophagen bei chronischen Hautentzündungen
… high CD64 expressing macrophages, through CD64 immunotoxin (full length antibody with a toxin), results in a resolution of chronic inflammations, confirms this. The immunotoxins of the first generation cannot be produced (however) in homogeneous quality and their biological and physicochemical …
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Herstellung und Charakterisierung rekombinanter Immunotoxine aus anti-PSMA single-chain Antikörperfragmenten zur Therapie des Prostatakarzinoms
… erfüllen. <br>Zur Konstruktion eines anti-PSMA-Immunotoxins wurde das scFv A5 rekombinant an eine verkürzte Form des Pseudomonas Exotoxin A (PE40) gekoppelt, dem die in der Natur vorkommende Zellbindungsdomäne Ia fehlt. Diese wurde durch A5 ersetzt, was zu einer Spezifität gegenüber …
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Understanding and Exploiting Viral Protein US28 During Human Cytomegalovirus Latency
… individuals. The second, a US28-specific immunotoxin, has previously been shown to directly kill latently infected cells. I show that new derivatives of this immunotoxin are more efficacious and can kill latently infected cells after a short incubation, paving the way for their use in ex …
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Konstruktion und Charakterisierung von rekombinanten Immuntoxinen zur Therapie von metastasierendem Pankreaskarzinom
… the application of monoclonal antibodies, immunotoxins or bispecific antibodies, is a promising alternative for treatment of pancreatic carcinoma. Since overexpression of EGF receptor (EGFR) in pancreatic tumors correlates with advanced clinical staging, tumor size and decreased survival of …
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The Steroid Sulfatase Inhibitor (p-O-Sulfamoyl) - Tetradecanoyl Tyramine (DU-14) Enhances Memory Retention in Passive Avoidance in Rats with Hippocampal Cholinergic Deficit
… male Sprague-Dawley rats were infused with the immunotoxin 192 IgG-saporin (SAP) into the medial septum, and tested using a step-through passive avoidance paradigm. There was a greater than 80% decrease in ChAT activity in the hippocampi of animals treated with SAP (p < 0.0001). There was also a …
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Antibody engineering for cancer therapy
… demonstrate the versatility of this antibody, an immunotoxin composed of AF-20 scFv fused to the highly cytotoxic recombinant toxin gelonin was constructed, and its in-vitro efficacy against three different tumor cell lines were evaluated. The IC50 of the AF-20 scFv-gelonin fusion was consistently …
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Engineering targeted proteins for intracellular delivery of biotherapeutics
… delivery in a uniquely specific manner. Immunotoxins were designed based on the plant toxin gelonin and targeted towards the canonical cancer-specific antigens: epidermal growth factor receptor and carcinoembryonic antigen. Using quantitative internalization flow cytometry matched with …
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Development of recombinant immunotoxins targeting overexpressed cell surface biomarkers trophoblast cell-surface antigen 2 and mesothelin for cervical cancer treatment
… therapeutic approaches, such as recombinant immunotoxins, which may offer targeted treatment options with reduced side effects compared to traditional therapies. In this study, we focused on developing a target-specific next-generation recombinant immunotoxin (rIT) to improve treatment …
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Antigen-specific depletion of autoreactive B lymphocytes in Multiple Sclerosis
… cloned without toxic moiety. The so-called ‘autoimmunotoxins’ MOG-ETA’ and PLP-ETA’, as well as their counterparts without toxin MOG and PLP, were efficiently expressed in bacteria. After their purification via IMAC and size exclusion chromatography (SEC), they all showed a high purity as …
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Herstellung, Expression und Charakterisierung von rekombinanten Antikörpern und Immuntoxinen gegen Phoma lingam, Sclerotinia sclerotiorum und Verticillium dahliae
… of antibody fusion proteins, so-called immunotoxins, that were specific for the pathogens and that carried fusion proteins that were toxic to the fungal pathogens. Thus, a prerequisite was the generation of pathogen specific antibodies. Further, these antibodies should maintain their …
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Establishment of enhanced protein purification strategies for recombinant immunotherapeutics
… and the bacterially expressed recombinant immunotoxin 13 H22(scFv)-ETA. IMAC was employed as the initial purification step to recover the full-length histidine-tagged recombinant proteins. The Ni2+ immobilized on the matrices in columns has a high affinity for Histidine incorporated, which …
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Generation and therapeutic evaluation of recombinant immunotherapeutics on CD64+ monocytic cell lines and polarized ex vivo differentiated human macrophages
… was used to generate; 1) a recombinant immunotoxin (rIT) through genetic fusion with the enzymatic domain of Pseudomonas aeruginosa exotoxin A (ETA`), or 2) a SNAP-tag fusion protein (FP) to allow chemical coupling of the antibody fragment to the cytostatic drug monomethyl auristatin F …
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Entwicklung, Optimierung und Charakterisierung anti-CD64-basierter Immuntherapeutika
Immunotoxins (ITs) commonly are composed of modified antibodies that are linked to a toxin either chemically or through the generation of a fusion protein. The antibody directs the IT to specific target cells where it binds to surface molecules and is internalized, allowing the cytotoxic component …
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Interactions of Proteins with Membranes: Insights from Molecular Dynamics Simulations
… for potential biomedical applications, e.g. immunotoxin therapies and targeted drug delivery; as nanopores for protein/DNA sequencing. Lastly, despite the variety in dimension, stoichiometry, structure, or number of protomers involved in pore forma- tion, PFTs share features concerning the …
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Development of novel RNA- and protein-based cancer therapeutics by either silencing of potential oncogenes or active restoration of a tumor suppressor gene
Cancer has become more and more prevalent over the last few years, reaching its highest levels per capita in the Western world in 2008. Mutations that produce dominant gain of function oncogenes and loss of function tumor suppressor genes have been discovered due to their alteration in human and …