Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Immunophenotyping"”.
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Large cell lymphoma : correlation of HIV status and prognosis with differentiation profiles assessed by immunophenotyping
… PBL with differentiation profiles assessed by immunophenotyping.
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Developing novel SNAP tag-based antibody fusion proteins directionally conjugated to fluorophores for immunophenotyping of acute myeloid leukemia
… chain reaction and cytogenetic analysis. Immunophenotyping by flow cytometry and immunohistochemistry can be used to identify specific proteins or antigens upregulated in AML. In low and middle-income countries, particularly Africa, AML diagnosis is primarily based on leukemic cells or …
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Immunophenotypic classification of canine malignant lymphoma in formalin-fixed, paraffin wax-embedded specimens using CD3 and CD79a cell markers
… on H&E sections, after standard processing. Immunophenotyping, using CD3 (T cell) and CD79a (8cell) markers, was carried out on the same sections. Intermediate grade lymphomas were the largest category (49.5%), with 16.5%high grade lymphomas. More than half (53.3%) of the lymphomas were of 8 …
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The influence of fixation and cryopreservation of cerebrospinal fluid on antigen expression and cell percentages by flow cytometric analysis
… pathologies and flow cytometry enables detailed immunophenotyping of cells present in CSF.However, the pauci-cellular nature of CSF, and the rapid cell death following sampling, incumbers the use of flow cytometric analysis of these samples. Immediate processing and analysis of CSF for flow …
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Molecular characterization of T cells across disease states in the central nervous system
… and glioblastoma (GBM) using a combination of immunophenotyping and functional approaches, including ultra low-input transcriptional profiling. First we use a series of unbiased approaches to identify functional differences between auto-reactive T cells derived from MS or healthy donors.
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Frequency of leukemia types and flowcytometric immunophenotypic characterization of acute lymphoblastic leukemia presenting at the university teaching hospital, Lusaka
… examination of blood or bone marrow smears only. Immunophenotyping is a further diagnostic tool that can be performed to diagnose Leukemias and in this study it was used to diagnose and sub type Acute Lymphoblastic Leukaemia (ALL).The aim of the study was to determine the frequency of the …
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Frequency of leukemia types and flowcytometric immunophenotypic characterization of acute lymphoblastic leukemia presenting at the university teaching hospital, Lusaka
… examination of blood or bone marrow smears only. Immunophenotyping is a further diagnostic tool that can be performed to diagnose Leukemias and in this study it was used to diagnose and sub type Acute Lymphoblastic Leukaemia (ALL).The aim of the study was to determine the frequency of the …
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INSIGHT ON ANGPTL3 IMPACT ON HEPATIC METABOLISM AND FUNCTION
… development was assessed through histology and immunophenotyping of blood, liver and bone marrow through flow cytometry. Results Angptl3KO mice exhibited persistent hypolipidemia regardless of feeding status, with higher TG clearance and a reduced hepatic lipoprotein production, without signs of …
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TRYPTOPHAN-DERIVED MICROBIAL METABOLITES PROMOTE NKT22 PRO-TUMORIGENIC FUNCTIONS IN COLITIS ASSOCIATED COLORECTAL CANCER PATIENTS
… cytometry, metagenomics, RNAseq, spatial immunophenotyping, transcriptomics, ex vivo and in vitro experiments were conducted. Metabolomics identified microbial-derived molecules activating iNKT cells. Mechanisms were studied using CAC murine models with or without functional iNKT cells. …
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Investigating immunosuppression as a disease modifying strategy in Parkinson's disease
… Chapter 3 discusses the optimisation of the immunophenotyping analysis using flow cytometry which is being used as an exploratory outcome measure in AZA-PD, and reports the baseline immune profile of the trial cohort compared to a matched control group, as well as the profile of a group of …
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Using genetic and genomic approaches to understand haematopoietic cellular biology and dysregulation in disease
… of haematological blood cell count traits and immunophenotyping and apply a targeted recall-by-genotype study to dissect the relationship between these traits, specifically neutrophil count and surface receptor expression. In summary, I demonstrate how describing biological mechanisms of …
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Immunological Characterization of Human Umbilical Cord Lining Derived Cells and their Therapeutic Application in a Diabetic Mouse Model
… lining mesenchymal cells (CLMCs). Preliminary immunophenotyping showed that CLECs but not CLMCs express significant levels of HLA-G isoforms and HLA-E, two MHC-Ib molecules with immunosuppressive functions which mediate immune tolerance during pregnancy. Further detailed immunological analysis …
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Microglial succinate receptor 1 (SUCNR1) sustains chronic CNS inflammation
… via ex vivo suspension mass cytometry (CyTOF) immunophenotyping. Mechanistically, I found that SUCNR1 has opposite effects on macrophages, where it attenuates their pro-inflammatory activation, compared to microglia, where it promotes the response to both pro-inflammatory stimuli and succinate …
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Spatial transcriptomic analysis of low-grade intestinal T-cell lymphoma and lymphoplasmacytic inflammatory bowel disease in cats
… molecular techniques for clonality and immunophenotyping. To identify the differences in gene expression in intestinal samples from cats with LGITL and IBD using spatial transcriptomics, formalin-fixed paraffin-embedded cat jejunum and ileum samples were used, six with a diagnosis of …
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A Molecular Cytogenetic Profile of the Chronic Myeloproliferative Disorders
… in stimulated samples. In addition to this, immunophenotyping of cells demonstrated that the lineage of propagated cells remained unaltered. By applying these improved techniques, CMPD samples and immortalised cell lines were then investigated for cryptic aberrations. Array comparative …
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Iron Dysregulation and Inflammation in Alzheimer’s Disease
… prior to the manifestations of clinical AD by immunophenotyping leukocytes and examining their cytokine production, as well as APP and Aβ expression, in subjects with mild cognitive impairment (MCI), a preclinical stage of AD. The production of the inflammatory cytokines IL-6, IL-8 and IL-10 …
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Myxoma Virus Therapy of Malignant Gliomas in Murine Syngeneic Models: Translating Barriers to Therapy
… in vivo had no change in treatment outcome. Immunophenotyping the glioma microenvironment found these gliomas were heavily infiltrated with microglia and macrophages, just as has been shown in human patients. Ablation of tumour-resident macrophages, not treatment-recruited macrophages, …
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Inhibition of adenosinergic signaling in the tumor immune environment: the effect of combination CD73 blockade and oHSV therapy on solid tumors
… infiltration and communication. Flow cytometric immunophenotyping and immunofluorescent imaging were utilized to confirm single cell sequencing predicted changes in tumor microenvironment. Sequencing data and in vivo blockade of myeloid cell recruitment into tumors revealed that this beneficial …
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Investigating the susceptibility of foreskin myeloid cells to ex vivo HIV infection
… cells from the foreskin epidermis (ii) Immunophenotyping and characterising foreskin LCs and “macrophage-like” cells using CD4+CCR5+ as proxy for HIV susceptibility, HLA-DR+CD80/86+ for maturation, and the mannose receptor, DC-SIGN and Siglec-1 as HIV attachment factors and (iii) …
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Study of anti-inflammatory functions of HDL in experimental mouse models
… capacity of HDL to neutralize LPS. Additional immunophenotyping showed that Lcat-/-, but not Apoa1-/- mice, have markedly increased circulating monocyte numbers as a result of an increase in ‘mildly pro-inflammatory’ Cd11b+LyCmid monocytes, whereas ‘highly pro-inflammatory’ Cd11b+LyChi …
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