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Showing 1 to 14 of 14 for “"Immunomagnetic separation"”.
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Density-shift Immunomagnetic Separation for Pathogen Retrieval from Complex Media
… as food samples. This thesis describes a novel immunomagnetic bead-based sample preparation method called density-shift immunomagnetic separation (DIMS). The DIMS method uses beads to capture target pathogenic bacteria and spatially separate them from both the background components of the sample …
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Optimization of automated immunomagnetic separation for the detection of escherichia coli 0157: H7 by quantitative polymerase chain reaction
… coii 0157 by qPCR assays. An improved automated immunomagnetic separation (AIMS) protocol was designed for the detection of Escherichia coil 0157:1-17 with specific, antibody coated, MyOneTm Dynabeads®. The new protocol was found to increase capture by approximately 60 % in comparison to the …
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Ανάπτυξη και κλινικές εφαρμογές μεθόδων μοριακής διαγνωστικής για την ανίχνευση κυκλοφορούντων καρκινικών κυττάρων στον καρκίνο του μαστού μέσω της έκφρασης της μαστοσφαιρίνης α
… the sensitivity of our methodology, we used immunomagnetic separation steps for the isolation of CTCs, as we have previously established in our laboratory and the selective isolation of mRNA from total RNA. To achieve that, we selected the previously established in our laboratory real-time …
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Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus
… The monoclonal antibody was employed in Immunomagnetic separation (IMS) protocol and was then coupled with Real-time PCR (q-PCR). IMS coupled to q-PCR was able to detect bacterial numbers in less than 3 hrs with a sensitivity of 1,000 CFU/g of oyster homogenate. In the second method …
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A Novel and Rapid Staphylococcus aureus Bacterial Identification Method Utilizing ImmunoMagnetic Beads and Single Cell Laser-Light Scattering
… method of <em>S. aureus </em>identification via ImmunoMagnetic Separation (IMS) and laser-light scattering identification technology. With this method, we hypothesized that anti-Protein A conjugated to magnetic DynaBeads could bind to surface Protein A on <em>S. aureus </em>from swab sample and …
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Application of Anti-H Monoclonal Antibodies to Develop Rapid Immunoassay for Vibrio vulnificus
… were utilized in the development of an immunomagnetic separation (IMS) protocol, which was then combined with rt-PCR to develop rapid method which can detect presence of V. vulnificus in oysters within 3 h with a sensitivity of 102 CFU/ml oyster homogenate. We have also used our anti V. …
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Separação imunomagnética de cistos teciduais e esporocistos de toxoplasma gondii pelo anticorpo monoclonal K8/15-15 e caracterização de sua proteína alvo por Western Blot e Imunoblot
… The current study aimed to develop a protocol of immunomagnetic separation (IMS) directed to T. gondii tissue cysts and soporocysts, and to isolate the protein recognized by the monoclonal antibody (mAb) K8/15-15. This was the first described mAb that is able to simultaneously recognize proteins …
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Detection of Giardia lamblia and Cryptosporidium parvum in the eastern oyster (Crassostrea virginica) harvested from the Great Bay Estuary
… and Cryptosporidium parvum in oysters. An immunomagnetic separation technique was utilized to capture the Giardia cysts and Cryptosporidium oocysts from oyster homogenate. This method resulted in the removal of enzymatic inhibitors and increased detection sensitivity. Primers designed to …
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Selezione e processazione di cellule Natural Killer da donatore aploidentico "KIR-ligand" incompatibile per l'immunoterapia adottiva di pazienti con Leucemia Acuta Mieloblastica ad alto rischio
… leukaphereses by using a double-step immunomagnetic separation system, consisting in depletion of CD3+ T cells followed by positive selection of CD56+ NK cells. CD56+ cells were enriched from 7,70% (1,26-11,70) to 93,50% (66,41-99,20) (median recovery 53,05% (30,97-72,85), median …
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A Bifunctional Nanocomposites Based Electrochemical Biosensor for In-field Detection of Pathogenic Bacteria in Food
… in foods. It was based on the techniques of immunomagnetic separation, enzyme labelling, and electrochemical impedance spectroscopy (EIS). This impedimetric immunosensor was capable of specifically detecting E. coli O157:H7 and S. Typhimurium within the range of 102 to 106 colony forming unit …
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Validation of dog treats and bones for the presence of Salmonella through in-plant analysis and inoculation challenge studies
… detection was performed by the BAX system and immunomagnetic separation (IMS) with Remel Salmonella agglutination kit for confirmation. Statistical analysis was performed using SAS program with a significance level of α = 0.05. Cooking reduced (P < 0.01) Salmonella of lamb lung cubes by 2.85 …
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Validation of dog treats and bones for the presence of Salmonella through in-plant analysis and inoculation challenge studies
… detection was performed by the BAX system and immunomagnetic separation (IMS) with Remel Salmonella agglutination kit for confirmation. Statistical analysis was performed using SAS program with a significance level of α = 0.05. Cooking reduced (P < 0.01) Salmonella of lamb lung cubes by 2.85 …
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Evaluation of Different Methods for the Detection of Mycobacterium bovis in Lymph Node Tissue
… specific or sensitive enough for this purpose. Immunomagnetic separation (IMS) gave a limit of detection result for M. bovis of 2.3 x 102 cfu/ml when spiked into PBS but this increased to 2.3 x 103 cfu/ml when spiked into negative LN tissue. IMS is not sensitive enough for the detection of …
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Surface Directed Monoclonal Antibodies against STEC Aid in the Reduction of Pathogen Detection Times from Food and Water
The diagnostic methods implemented at the Canadian Food Inspection Agency for the detection of Shiga toxin producing E. coli (STEC) are time consuming and tedious, taking up to 5 days before a positive sample can be confirmed. The goal of this project was to streamline the detection procedure for …