Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 67 for “"Immunohistochemistry ( IHC)"”.
-
PI3K/Akt activation is critical for early hepatic regeneration after partial hepatectomy
… by a paucity of F4/80+ cells present by immunohistochemistry (IHC). Additionally, PI3K inhibition led to an aberrant hepatocyte architecture characterized by vacuolization, lipid deposition, and glycogen accumulation. My data demonstrate that PI3K/Akt pathway activation plays a critical …
-
Assessing Inter-rater Agreement for Compositional Data
… is focused on pathology, where pathologists rate immunohistochemistry (IHC) assays using compositional scores. To enhance the use of this methodology and help with the design of future agreement studies, an R Shiny package designed for the IHC agreement analysis is developed.
-
Prevalence of bovine viral diarrhea virus subspecies among persistently infected positive samples submitted to a diagnostic laboratory from cattle in the United States
… infection via antigen capture ELISA (ACE) or immunohistochemistry (IHC). This study was comprised of 1,093 samples from 21 states, with a majority of samples from Kansas, Kentucky, Oklahoma, and Texas. Positive samples were submitted to a university diagnostic laboratory and segregated into …
-
Genetic Approach to Discover ARMC4 as a Novel NF-κB Negative Regulator and Tumor Suppressor in Colorectal Cancer
… with the p65 subunit of NF-κB. Importantly, immunohistochemistry (IHC) data exhibited much lower ARMC4 expression level in CRC patient tumor tissues compared to normal tissues, indicating that ARMC4 may function as a tumor suppressor in CRC. To conclude, my important findings for the first …
-
Influence of Preoperative Transcatheter Arterial Chemoembolization on Gene Expression in the HIF-1α Pathway in patients with Hepatocellular Carcinoma
… –associated genes were examined using immunohistochemistry (IHC) and real-time RT-PCR, respectively. Preoperative TACE was significantly associated with increased 2-year recurrence rate (80 vs. 36%, P=0.00402) and shorter disease-free survival (DFS) time (11.9 vs. 35.7 months, …
-
Universal Lynch Syndrome Screening in Newly Diagnosed Colorectal Cancer: Impact of an Alberta-wide Screening Program
… Universal tumor screening in all new CRCs using Immunohistochemistry (IHC) to assess for loss of MMR-protein expression allows for identification of individuals at increased risk for underlying LS who would benefit from genetic testing. This thesis reports the results of one study performed with …
-
Halting the neural progression of cancer
… were conducted to test this hypothesis. Immunohistochemistry (IHC) was used to examine neuronal density in tissue sections from mice implanted with melanomas that overexpressed SEMA3F compared to control tumors or in mice lacking Nrp2 compared to wildtype (WT) mice with both copies of the …
-
Differential membrane-type matrix metalloproteinase expression in phenotypically defined breast cancer cell lines: Comparison of MT-MMP expression in environmentally-challenged 2D monolayer cultures and 3D multicellular tumour spheroids
… and MCTS models using immunofluorescence (IF), immunohistochemistry (IHC) and western blot (WB). MT1-MMP expression was rapidly and irreversibly up-regulated in MCF-7 breast cancer cells under conditions of stress (hypoxia and autophagy) compared to normal conditions suggesting an important role …
-
Deep Learning in Oesophageal Cancer Development: Integrating Multi-Stain Histopathology Images of the Capsule Sponge
… is often absent, empty regions dominate, and immunohistochemistry (IHC) slides contain control tissue that complicates automated analysis. This thesis develops and evaluates a computational pipeline to address these challenges and enable scalable risk stratification for BE. First, I present …
-
Cancer Cartography: Mapping Cancer Evolution in Tissue Space
… stages of progression. Integration of histology, immunohistochemistry (IHC), and targeted in situ gene expression allows for the phenotypic characterisation of clones in distinct microanatomical niches. Subsequent analyses across various stages of breast cancer, including carcinoma in situ, …
-
The Immune Microenvironment of Microsatellite Instable Endometrial Cancer
… gene and pathway level. Fluorescent multiplexing immunohistochemistry (IHC) was used to evaluate differences in immune cell populations using tumor specimens from these two groups followed by automated multispectral imaging and analysis to visualize and quantify staining in the tumor epithelial …
-
Investigating The Metabolic Progression of Glioblastoma With Hyperpolarized Magnetic Resonance
… and <em>ex vivo </em>protein expression with immunohistochemistry (IHC) were measured at several time-points throughout tumor progression (tumor development, regression, and recurrence).</p> <p>Hyperpolarized MRS was capable of detecting significant changes in pyruvate-to-lactate conversion …
-
Evaluation of telomerase activity and telomerase inhibitors in Head and Neck cancer
… was analysed in paraffin embedded tissue using immunohistochemistry (IHC). Whilst telomerase was detected in cell lines, no telomerase activity was detected in saliva samples from patients but was detectable in IHC specimens. The second part of the study focused on the pharmacological evaluation …
-
The Role of TBX3 In Early Breast Cancer Progression
… TBX3 expression in early stage breast cancers by immunohistochemistry (IHC) revealed high expression in low-grade lesions. Within a second non-high-grade cohort, there was an association between TBX3 expression in the pre-invasive ductal carcinoma in situ (DCIS) and size of the invasive focus. …
-
Acute Physiological Effects of Brain-Generated 17β-Estradiol in the Rodent Primary Auditory Cortex
… in the rodent auditory forebrain. First, immunohistochemistry (IHC) was used to characterize neocortical expression of the estrogen synthetic enzyme aromatase in adult male rats. Aromatase was expressed by a large population of neurons in medial prefrontal cortex (mPFC), primary …
-
The development of novel diagnostic countermeasures for Rift Valley fever virus
… provide working protocols for western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) that use monoclonal or polyclonal antibodies against key RVFV antigens. These tools can be applied to pathogenesis research and used in the development of vaccine and therapeutic …
-
Thermal Ablation Alters The Tumor Microenvironment Activating A Robust Immune Response.
… with ablation. Gene microarray analysis and immunohistochemistry (IHC) for presence of immune cell subtypes were performed on the tissue samples. Prospectively, blood was collected from patients 13 patients who underwent renal ablation for renal cell carcinoma for stage T1a (less than 4cm) at …
-
Glutamate Imaging of Mouse Models of Neurodegeneration
… were correlated with results from 1HMRS and immunohistochemistry (IHC). The primary IHC antibodies that were investigated include markers of phosphorylated tau protein, synapse density, neuron density, glial cell reactivity, a glutamate transporter, and an NMDA receptor. GluCEST contrast …
-
Molecular Characterization of Early Dedifferentiation in Newt Forelimb Regeneration
… these genes in the regenerating newt limb using immunohistochemistry (IHC) and in situ hybridization (ISH). Finally, we performed a functional analysis on β-catenin, wntless, dapper, and Tβ4 by overexpressing these genes in mouse myoblasts to examine their effects on differentiation and potential …
-
Nicotine Alters Gene Regulatory Networks in Dopaminergic Neurons in the Ventral Tegmental Area
… polymerase chain reaction (RT-qPCR) and immunohistochemistry (IHC) to investigate the number of GABA, DA, and glutamate neurons, we found that the middle region of the VTA contained a more diverse population of neurons and the bottom region showed a higher expression of DA markers. Our …
Page 1 of 4