Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Immunogold labelling"”.
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The study of cell surface components on Porphyromonas gingivalis
… of the native PgAg1 molecule was demonstrated by immunogold labelling of intact P. gingivalis cells using antibodies raised to the recombinant protein (arPgAg1). The recombinant protein was purified to homogeneity, and the native PgAg1 protein partially purified from P. gingivalis. The …
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Investigation and development of techniques for the characterisation of the synthetic/biological interface
… in promoting cell spreading on surfaces. The immunogold labelling technique indicated that there were small, but noticeable differences, between the distribution of proteins on a range of surfaces. This study has shown that surface analysis techniques have different sensitivities in terms of …
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Influence of ApoE polymorphism on synaptic morphometry during aging in the dentate gyrus of ApoE knockout and human ApoE transgenic mice
… and display the highest syn/nrn and glutamate immunogold labelling in presynaptic boutons and dendrites of the MML, compared to hApoE3 and hApoE4 mice. In AD patients, such effects of the ?2 allele may act as a synaptic 'reservoir' and delay the onset of AD symptoms. Thus these lines of hApoE …
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Novel approaches to assess cellular interactions and their role in the pathology and treatment of lymphoproliferative disorders
… a 3D tumour microenvironment (TME)-like setting. Immunogold labelling of specific proteins within neoplastic lymphocytes was performed to allow visualisation of protein localisation changes in response to signal inhibition at the ultrastructure level. Results: This study tested inhibitors …
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Morphological, biochemical and molecular characterization of desiccation-tolerance in cyanobacterium Nostoc commune var. Vauch
… DRH1 through light and electron microscopy, immunogold-labelling and staining with dyes, revealed changes in the pattern of differentiation in glycan micro-structure, as well as localized shifts in pH, upon rehydration of desiccated field material. A Ca/Si rich external (pellicular) layer of …
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The colonisation of treated water systems by <i>Legionella pneumophila</i>
… formation. Examination of the biofilms using immunogold labelling techniques demonstrated that cells of L. pneumophila were present within the biofilm as microcolonies, this could indicate that the pathogen could grow extracellularly by receiving nutrient from the bacterial consortium. …
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Role and regulation of glutamate dehydrogenase activity in Bacteroides fragilis bf1
… fragilis cell-surface was further confirmed by immunogold labelling of B. fragilis cells, followed by electron microscopy. The cell-surface localisation of this enzyme may facilitate the utilisation of glutamate, derived from peptides incorporated from the external milieu.
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Phytoextraction of heavy metal from contaminated soils using genetically modified plants
… tobacco plants was confirmed by immunoblot and immunogold analyses. Immunoblot analysis indicated that high accumulation of the recombinant proteins was achieved in tobacco plants transformed with the cV-ChMTII-GFP construct, whereas the lower protein accumulation was observed for the S-ScMTII. …
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Virulence and pathogenic mechanisms of <i>Legionella pneumophila</i>
… the changes in cellular responses using immunolabelling techniques and flow cytometry. Difficulties with obtaining consistent, sequential samples did not allow a full interpretation of the results but the technique showed promise for such studies. <br></br><br></br>The tissue destructive …
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Receptor-mediated endocytosis of low density lipoproteins in aortic endothelial cells
… antibody (IgG-C7), localised by indirect immunogold labelling, gave similar results when applied to the above cells. This was taken as strong corroborative evidence that the LDL receptor distributions as determined by colloidal gold-conjugated LDL were correct. It is suggested that the …
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Morphological and Physiological Studies of the Carbon Concentrating Mechanism in Chlamydomonas reinhardtii
Chlamydomonas reinhardtii possesses a single-cell-based CO2-concentrating mechanism (CCM). The CCM is an important element of algal photosynthesis, metabolism, growth and biomass production, which works by increasing the concentration of inorganic carbon (Ci) in the pyrenoid, a dense RuBisCO-packed …