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Showing 1 to 19 of 19 for “"Immunoelectrophoresis"”.
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Tissue Hypertrophy: A Quantitative Adaptational Strategy During Cold Acclimation in Channel Catfish and Green Sunfish (Immunoelectrophoresis, Heart, Liver, Protein Synthesis, Degradation)
1. Livers of 15 C acclimated channel catfish (CCF) exhibit cellular hypertrophy compared to livers of 25 C acclimated catfish as shown by doubling of hepatosomatic index (HSI) and total liver protein. No change occurs in protein concentration or total liver DNA. The same pattern was observed for …
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Antigenic Variation in Candida Albicans
… Candida albicans have been compared by crossed immunoelectrophoresis, tandem-crossed immunoelectrophoresis and classical immunoelectrophoresis. Qualitative and quantitative differences between the two growth forms have been observed. One unique antigen to the yeast phase was found. Possible …
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Ο ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΤΗΣ ΠΡΩΤΕΙΝΗΣ ΡΟΥΜΠΙΣΚΟ (RUBISCO) ΣΤΑ ΦΥΤΑ
… FOR THE MAXIMIZATION OF ITS EXTRACTION. ROCKET IMMUNOELECTROPHORESIS TECHNIQUE SEEMED TO BE THE MOST ACCURATE AND APPROPRIATE METHOD BECAUSE IT MAKES POSSIBLE THE QUANTITATION OF THE TOTAL EXTRACTABLE RUBISCO AND NOT ONLY THE ENZYMATICALLY ACTIVE, IN MIXTURE WITHOUT A PREVIOUS PURIFICATION OF …
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Studies of Culture-Derived Soluble Babesia Bovis Antigens: Purification, Characterization and Application in Serodiagnosis
… Isolated antigens were identified by crossed immunoelectrophoresis.
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Isolation and Immunochemical Characterization of Soluble Anaplasma Marginale Antigen (Rickettsia, Hemoparasite, Disease, Elisa)
… anionic as demonstrated by its fast mobility in immunoelectrophoresis, crossed immunoelectrophoresis and non-denaturating polyacrylamide gel electrophoresis with electrotransfer blotting analysis. According to gel filtration sieve chromatography the antigen was located primarily in the region of …
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Part One: Investigation of the Immunogenicity of a Spin Label Probe. Part Two: An Electron Paramagnetic Resonance Study of Dinitrophenyl-Binding Myelomas and Hybridomas Using Spin Labelled Probes
… observed with either capillary precipitin tests, immunoelectrophoresis, or epr studies. Fluorescamine was used to quantitate the substitution of the spin probe on BSA, and results indicated the spin probe was leaching from the carrier. Epr studies confirmed the leaching phenomenum. Formation of an …
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Immunodiffusion and immunoelectrophoretic studies on Trypanosoma lewisi (Kent) during the course of an infection in the albino rat, Rattus rattus
<p>Immunoelectrophoresis revealed an antigen-antibody response between 4 day metabolic products and 8, 12 and 16 day sera and between 4 day trypanosomal extract and 16 day serum.</p><p>Metabolic products from trypanosomes incubated at room temperature do not appear to be antigenic.</p><p>The …
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Isolation and Characterization of Blood Lipoproteins From Dairy Cows Fed Control or High Grain Diets (Lactation, Feeding, Lipids)
… from disc polyacrylamide gel electrophoresis, immunoelectrophoresis, and double immunodiffusion of lipoprotein fractions, and SDS polyacrylamide gel electrophoresis of their apoproteins, indicated that high density lipoproteins 1 are a mixture of low and high density lipoproteins rather than a …
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A study of treponemal antigens
… as demonstrated by immunodiffusion, crossed immunoelectrophoresis, ELISA, and Western blots. An immunodiffusion test using treponemal Triton-extracts and antisera against five treponeme species was developed for the routine identification of oral treponemes that were in either pure or mixed …
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Purification of nicotinamide adenine dinucleotide phosphate- specific glutamate dehydrogenase from Chlorella sorokiniana and partial characterization of its physical, kinetic, and immunological properties
… Ouchterlony double diffusion analysis. "Rocket" immunoelectrophoresis has been used to quantify the amount of antigen present in samples of the purified enzyme.
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Study of macroglobulinaemia in Trypanosoma equiperdum infections of the rabbit
… of 195 IgM, measured by gel diffusion and rocket immunoelectrophoresis were shown to increase approximately ten times at the peak of the Trypanosoma eguiperdum infections in the rabbit. The serum IgG. concentrations, measured by gel diffusion increased more than seven times.aver the infection …
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Use of immunological procedures to measure rate of accumulation and degradation of inducible NADP-specific glutamate dehydrogenase during cell cycle of synchronous Chlorella
… NADPGDH has a maximum half-life of 1.6 h. Rocket immunoelectrophoresis was used to show that enzyme catalytic activity and antigen increased in a parallel fashion during the cell cycle of fully-induced cells. These data suggest that the cell cycle catalytic activity pattern reflects de novo …
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Immunological responses of fishes to glochidia of freshwater mussels
… by glochidia before attachment to host fish. Immunoelectrophoresis (IEP) was used to compare precipitation patterns of host and non-host fishes and to determine whether IEP can be used for identifying suitable hosts for a species of mussel. Although there were subtle differences observed …
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Metabolism of supplemental iron by hepatic parenchymal and sinusoidal cells of the neonatal pig
… quantity of cellular ferritin was measured by immunoelectrophoresis. The amount of cellular iron associated with ferritin was assessed by ion exchange chromatography. All three types of liver cells accumulated ferritin in response to Fe-dextran treatment. Higher concentrations of ferritin-iron …
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Immunological and biochemical techniques in the analysis of tear proteins
… in the analysis of lens extracts. Counter immunoelectrophoresis, an immunodiffusion assay, was then applied to the analysis of albumin, lactoferrin, IgA and IgG in the resultant eluates.
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Purification and characterization of Clostridium perfringens iota toxin
… By using C. spiroforme antiserum and crossed immunoelectrophoresis (crossed IEP), we found two cross-reacting antigens in C. perfringens type E supernatants. C. perfringens types A, B, C, and D, which do not produce iota toxin, did not cross-react with C. spiroforme antiserum. To determine if …
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The Isolation and Characterization of Soluble Brain Proteins
… antigenic components could be demonstrated by immunoelectrophoresis; most were present in extracts of other tissues as well. The various antigens, most of which were in the pH 5 soluble fraction, could be separated according to their electrophoretic mobility by zone electrophoresis on starch. …
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The Biogenesis of Peroxisomal Catalase in Rat Liver
… proved to be monospecific by immunodiffusion and immunoelectrophoresis. The standard methods of immunoprecipitation were modified to allow the specific precipitation of a minor component (catalase) out of a crude cell homogenate and fractions thereof. The use of siliconized, glass, conical tubes …
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Ο ΒΑΘΜΟΣ ΓΛΥΚΙΩΣΗΣ ΤΗΣ Α1-ΟΞΙΝΗΣ ΓΛΥΚΟΠΡΩΤΕΙΝΗΣ ΤΟΥ ΟΡΡΟΥ ΩΣ ΔΕΙΚΤΗΣ ΣΕ ΚΑΚΟΗΘΗΝΕΟΠΛΑΣΙΑ
ΣΤΗΝ ΠΑΡΟΥΣΑ ΔΙΑΤΡΙΒΗ ΜΕΛΕΤΗΘΗΚΕ Ο ΒΑΘΜΟΣ ΓΛΥΚΙΩΣΗΣ ΤΟΥ ΜΟΡΙΟΥ ΤΗΣ Α1- ΟΞΙΝΗΣ ΓΛΥΚΟΠΡΩΤΕΙΝΗΣ (Α1AGP) ΜΕ ΤΗΝ ΜΕΘΟΔΟ ΤΗΣ ΑΝΟΣΟΗΛΕΚΤΡΟΦΟΡΗΣΗΣ ΣΥΓΓΕΝΕΙΑΣ ΔΥΟ ΔΙΑΣΤΑΣΕΩΝ ΜΕ CONCANAVALIN A ΣΤΗΝ ΠΡΩΤΗ ΔΙΑΣΤΑΣΗ. ΜΕΛΕΤΗΘΗΚΕ ΤΟ ΑΝΟΣΟΗΛΕΚΤΡΟΦΟΡΗΤΙΚΟ ΠΡΟΤΥΠΟ ΤΗΣ Α1AGP (PATTERN) ΣΕ ΑΣΘΕΝΕΙΣ ΜΕ ΝΕΟΠΛΑΣΜΑΤΑ …