Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 197 for “"Immunocytochemistry"”.
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Immunocytochemistry, Mass Spectrometry and Microengineering for the Study of Cell Signaling
This is an integrative study that combines information obtained by the use of selective probes and direct chemical profiling of individual neurons for the investigation of physiological and behavioral aspects of cell signaling mediated by neuropeptide transmitters in the brain of Aplysia …
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Immunocytochemistry Of Rectal Cancer Circumferential Margins In The Assessment Of Micrometastatic Disease
… the circumferential margins by using automated immunocytochemistry (ICH) with MNF116 and CK5D3 monoclonal antibodies.The International Union Against Cancer (IUAC) criteria were applied objectively to classify the micrometastatic disease in to MM and ITC. This was compared with Haematoxylin and …
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ULTRASTRUCTURE, IMMUNOCYTOCHEMISTRY, AND BIOINFORMATICS OF SPORE DEVELOPMENT IN THE MOSS PHYSCOMITRELLA AND THE HORNWORT DENDROCEROS
Spores are single-celled dispersal units surrounded by a wall of the highly resistant biopolymer sporopollenin. All land plants produce spores. Spore development is described in <italic>Physcomitrella patens</italic>, a moss with single-celled spores, and <italic>Dendroceros</italic>, a hornwort …
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Stimulated Emission Depletion (STED) Microscopy and Pacific Orange Dye Optimization For H9C2 Cox-1 Imaging Via Indirect Immunocytochemistry
<p>The fundamental barrier of traditional microscopy has always been the Abbe limit. Diffraction has served to limit image production and microscopic investigation on the sub-cellular level, greatly hindering microbiology and other forms of study. Stimulated Emission Depletion microscopy is one of …
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THE SPECIAL WALLS AROUND GAMETES IN CERATOPTERIS RICHARDII AND AULACOMNIUM PALUSTRE: USING IMMUNOCYTOCHEMISTRY TO EXPOSE STRUCTURE, FUNCTION, AND DEVELOPMENT
… RICHARDII AND AULACOMNIUM PALUSTRE: USING IMMUNOCYTOCHEMISTRY TO EXPOSE STRUCTURE, FUNCTION, AND DEVELOPMENT MAJOR PROFESSOR: Dr. Karen S. Renzaglia Gametes are arguably the most important cells formed during the sexual life cycle of plants. The ancestral condition of gametes in land plants …
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Steroidogenic cell populations in follicles of the chicken ovary
… in the theca layer of preovulatory follicles by immunocytochemistry for P450 steroidogenic enzymes and to measure steroid production by isolated theca cells in a short term cell incubation (Experiment 1); (2) to localize cell populations containing P450 steroidogenic enzymes in small follicles by …
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IMMUNOPROFILE IN EFFUSION CYTOLOGY
… formulate such a diagnosis. In human medicine, immunocytochemistry of effusion cytology has now standardized procedures that provide reliable insights into various diagnostic dilemmas. OBJECTIVE: To describe the method of immunocytochemistry in effusion cytology and to estimate the value of a …
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Identification, Characterization and Mechanism of the Estrogen Regulation of the Gene Encoding the Sodium /Hydrogen Exchanger -Regulatory Factor (nhe-Rf)
… of the NHE-RF gene using two model systems. Immunocytochemistry was used to visualize NHE-RF in MCF-7 cells. Secondly, we created adenovirus that express the wild type or anti-sense NHE-RF RNA. By infecting cells with the adenovirus we may overexpress or functionally ""knock out"" the NHE-RF …
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Biochemical and Immunocytochemical Characterization of Canine Corneal Cells Cultured in Two Different Media
… or Epilife®. First passage cells were fixed for immunocytochemistry and prepared for PCR. Immunocytochemical staining for pancytokeratin, vimentin, and E-cadherin was evaluated, and immunofluorescence for zonula occludens-1 was attempted. Amplification of cytokeratin 5 (CK5) mRNA was assessed by …
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불멸화 유전자 도입을 통한 베타 세포주 구축
… virus의 transduction 16 5. Soft agar assay 16 6. Immunocytochemistry 16 7. BrdU incorporation assay 16 8. 단일 세포 선별 18 9. 인슐린 정량 18 10. RT-PCR 18 11. MTT assay 19 Ⅲ. 결과 23 Ⅳ. 고찰 49 Ⅴ. 결론 54 참고문헌 55 ABSTRACT 59"
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FRACTURE HEALING IN A RABBIT MODEL: A STUDY OF OSTEOCALCIN LOCALISATION
Immunocytochemistry was used to localise osteocalcin during fracture healing in tissue sections of adult rabbit tibiae. A peroxidase anti-peroxidase technique and an indirect peroxidase technique were assessed on newly developing bone in human fracture callus. Three antibodies which cross-reacted …
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Expression Profiles of Molecules Involved in Antigen Presentation in Gangliosides-stimulated Primary Astrocytes from Rat brain
… the surface expression of MHC II by FACS and immunocytochemistry analysis. Gmix treatment failed to induce it in both FACS and immunocytochemistry analysis, in contrast to IFN-gamma treatment. This imply that Gmix could induce the mRNA expressions of CIITA and MHCII, but they could not induce …
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Copper ionophores induce autophagy in melanoma cells.
… were investigated against melanoma. By using immunocytochemistry, Western Blotting and supravital staining, it was confirmed that both ionophores induce autophagy, and furthermore, drive the process to completion. In addition, when tested for viability with the autophagy inhibitor …
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Clinical review and experimental evaluation of tumour M2-pyruvate kinase in pancreatic cancer
… M2-PK in pancreatic cancer cell was studied by immunocytochemistry. The clinical review has shown that Tumour M2-PK is not an organ-specific marker of GI cancer but is elevated with positive predictive value of 86–88% in gastro-oesophageal and colorectal cancers. In pancreatic cancer the …
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Choline Acetyltransferase and Carnitine Acetyltransferase Activity in Human Spermatozoa During Capacitation
… in human spermatozoa as assessed by fluorescent immunocytochemistry was compared with ChAT and CaAT activity determined by enzymatic methodologies. Increasing proportions of sperm exhibited ChAT immunoreactivity along the equatorial region with a concomitant decrease in ChAT reactivity in the …
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Mechanism of Insulin Release From Islets of Langerhans in a Rat Model of Normal Infants and Infants of Diabetic Mothers
… expression of GLUT1, GLUT2, and glucokinase by immunocytochemistry in the isolated islet and Western blot on the whole pancreas. The IDM had significantly lower mean body weights at all ages studied (p $<$ 0.01, n $\ge$ 34); significantly higher mean plasma glucose concentration at day $-$1 (p …
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Polyethylene glycol copolymer nanocarriers: Biocompatibility, uptake and intracellular trafficking in neurons
… and neuronal cell line, as assessed using immunocytochemistry and confocal microscopy. The other three nanomaterial systems are surface functionalized nanocarriers (SFNCs) made of fluorescently-labeled PEG copolymers without the magnetic core. One system is derivatized by …
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Appetite Regulatory Neural Circuitry in the Prenatal Testosterone Treated and Obese Ewe
… treatment, or in diet-induced obese ewes. Using immunocytochemistry, we found that prenatal T-treated ewes showed increased expression of the appetite stimulatory peptide, AgRP, but not the inhibitory peptide, POMC, consistent with the increased risk of obesity in these animals. In contrast, …
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DIFFERENTIATION OF NEURAL STEM CELL USING SMALL MOLECULES IN 2D AND 3D CULTURE SYSTEM
… polymerase chain reaction (RT-qPCR) and immunocytochemistry (ICC), respectively. This study will show the effect of bioactive compounds on hNSCs differentiation in 2D and 3D culture systems.
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Characterization of TvDMC1 and TvSOD6 expression and function in trichomonas vaginalis
… in the standard lab strains Tl and G3 and immunocytochemistry studies showed that HA-tagged SOD6 protein localizes in the cytoplasm. Lastly, we found that SOD protein abundance increased in the CDC085 strain compared to Tl and G3, especially under aerobic conditions.</p>
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