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Showing 1 to 20 of 42 for “"Immunoblots"”.
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Apoptosis Pathways: Presence and significance in Ejaculated Human Spermatozoa
… evaluation of phosphatidylserine translocation, immunoblots, and immunocytochemistry were used in this investigation.</p> <p>Of the pro-apoptosic treatments, only staurosporine resulted in elevated caspase activity in patients and donors. Differences in caspase activity were found between donor …
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Immunological Characterization of Membrane Skeletal Proteins in "Tetrahymena pyriformis" GL
… residues: band A and C, and a 23 kDa protein. Immunoblots were performed to characterize the number and size of immunoreactive proteins in total cellular protein as opposed to previous studies which only probed cortical proteins. Furthermore, immunoblots were performed to test the specificity …
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Molecular characterization of phosphoribulokinase and rubisco activase from Chlamydomonas reinhardtii
… five higher plant species and Chlamydomonas. Immunoblots also revealed substantial amounts of phosphoribulokinase protein in Chalmydomonas mutant strain 12-2B, but none in mutant strain F-60. Chlamydomonas and spinach cDNA clones containing the entire phosphoribulokinase coding region were …
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Influenza hemagglutinin expression in Nicotiana tabacum and Nicotiana benthamiana.
… screened for protein production using immunoblots to verify HA3 protein expression. A tobacco mosaic virus (TMV) vector (pJL36) was also produced expressing HA3. Recombinant constructs of the TMV vector were successfully screened using PCR. This work indicates the potential use of …
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Developmental Expression of Rat Target of the Antiproliferative Antibody (rTAPA) Protein in the Brain
… with the stabilization of cellular contacts. On immunoblots of the brain, rTAPA is present in higher levels than any other tissue examined: muscle, tendon, peripheral nerve, cartilage, liver, kidney, skin, and testicle. Immunohistochemical methods were used to define the distribution of rTAPA in …
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Exploring the Functional Interaction Between CaMK-II and p53
… transduction of p53 shRNA and verified with immunoblots and immunostaining assays. Next, the localization of CaMK-II and the cell growth rate in these cells was determined. In wild type HEK cells, catalytically inactive CaMK-II inhibited cell growth, which is consistent with previous studies …
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Isolation and characterisation of synaptic vesicles from mouse brain
… gegenüber klassicher Methoden. An Hand von Immunoblots und Elektronenmikroskopischen Aufnahmen kann die Sauberkeit der gewonnen Vesikel Fraktion gezeitgt werden. Durch den Einsatz an gerimgem Ausgangsmaterial, können synaptische Vesikel auf Transgenen Mäusen isoliert und die …
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Type IV Pili-Dependent Secretion of Biofilm Matrix Material Proteins in Clostridium perfringens
… protein CPE0515. HA-tagged CPE0516 qualitative immunoblots showed that, unlike CPE0515, oligomerization of CPE0516 is not occurring, and that this protein likely forms a heat stable dimer. Overall, the data did not allow us to construct a T2SS model, since there were not enough proteins revealed …
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Isolation and detection of bean yellow mosaic, clover yellow vein and peanut stunt viruses from Trifolium L. species
… used to detect viruses. In terms of specificity, immunoblots were equal or superior to i-ELISA. The TIBA should be useful in support of breeding and plant pathology studies as it is simple and rapid, and is less laborious and less expensive than i-ELISA.
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Characterization of the Sinorhizobum Meliloti Chotaxis System
… system from the enteric paradigm. Quantitative immunoblots determined the cellular amounts of chemotaxis proteins. Overall, chemotaxis protein levels were approximately 10-fold lower in S. meliloti compared to B. subtilis and E. coli. Focusing on cellular stoichiometric ratios, S. meliloti …
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Μελέτη του ρόλου της σεροτονίνης και της σηματοδότησης μέσω Wnt και BMP μονοπατιών στον έλεγχο της οστεοβλαστικής δραστηριότητας σε ασθενείς με αγκυλοποιητική σπονδυλίτιδα
… των επιπέδων του phospho-CREB με western immunoblots. Τα επίπεδα σεροτονίνης ορού ήταν στατιστικά σημαντικά χαμηλότερα στους ασθενείς με AΣ σε σύγκριση με τους υγιείς μάρτυρες (mean ± SEM ng/ml: 122.9 ± 11.6 vs 177.4 ± 24.58, p=0.038) και τους ασθενείς με ΡΑ (mean ± SEM ng/ml: 244.8 ± …
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Antioxidant responses of pea (Pisum sativum L.) protoplasts
… of glutathione reductase protein, as judged by immunoblots, did not correlate with the observed changes in enzyme activity. No change in the de novo synthesis of glutathione reductase occurred over the same period as a consequence of paraquat application. A mechanism, unrelated to oxygen free …
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Isolation of the Cytosolic C-Terminus Segment of Mammalian Kca Channel a Subunit
… expressed BK<sub>ca</sub>from a GH3 cells. Immunoblots using lystes of GH3 cells unexpectedly revealed a 70 kDa BK ‘tail’ like protein because it reacted with anti-BK antibodies. We determined that the high-speed supernatant fraction of these GH2 cell lysates was enriched with this 70 kDa …
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Endothelin-1 Induced Phosphorylation of ERK1/2 in Bovine Corneal Endothelial Cells
… blotting technique. Densitometry analysis of immunoblots revealed differential phosphorylation patterns in confluent and sub-confluent cultures. The pERK1/2 levels were significantly increased at 15 min and 24 hrs after post incubation with ET-1, whereas following the initial rise levels …
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The Effect of Endothelin-1 on the expression of CDK Inhibitors p21 & p27 in Bovine Corneal Endothelial Cells
… to Endothelin-1. Densitometry analysis of immunoblots revealed an increase in the expression of p27kip1 in confluent cell cultures when compared to sub-confluent, dividing cells. p21cip1 was almost undetectable in sub-confluent, actively dividing cultures. Immuno fluorescent analysis …
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Characterization of a fatty acid elongase condensing enzyme by site-directed mutagenesis and biochemical analysis
… protein expression for each mutant. Analysis of immunoblots indicated that those mutants with little to no activity, with the exception of T130A and Q203A, had x comparable protein expression to the wild-type. Further research included the solubilization of the His6-ELoA fusion protein and …
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Identification and Analysis of Peanut and Soybean Cross Reacting Allergens in Korean Children Sensitive to Peanut
… for specific IgE binding to peanut or soybean immunoblots. SDS-PAGE and peanut-IgE immunoblotting with soybean-inhibited peanut allergy patient sera and non inhibited patient sera were performed. The spot membrane containing the peptides was analysed by Procise 492 cLC Protein sequencer …
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H36-alpha7 is a novel integrin alpha chain that is developmentally regulated during skeletal myogenesis
… with azacytidine or transfection with MRF4. Immunoblots and immunofluorescence demonstrate that the H36-$\alpha$7 chain is associated with integrin $\beta$1. Affinity chromatography demonstrates that H36-$\alpha$7$\beta$1 selectively binds to laminin. The expression of H36-$\alpha$7 on …
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REGULATION OF ALGINATE AND PSL POLYSACCHARIDE EXPRESSOIN IN CLINICAL STRAINS OF PSEUDOMONAS AERUGINOSA
… in mucoid strains of P. aeruginosa. Psl immunoblots and ELISAs showed that the alginate regulator AmrZ represses Psl production. AmrZ is a DNA binding protein and is thus a candidate for transcriptional repression of the psl operon. DNA binding assays show that AmrZ does bind upstream of …
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