Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 125 for “"Immunoassays"”.
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Multiplexed nanoparticle-based immunoassays
Multiplexed immunoassays have been explored using the fluorescent and luminescent properties of fluorophores and nanoparticles. Epi-fluorescence microscopy, confocal laser scanning microscopy and programmable array microscopy were used to detect signals from mixtures of conventional organic …
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Immunoassays by flow injection analysis.
A home-built flow injection analysis (FIA) system was fully automated using an Apple IIe microcomputer, and subsequently used as a versatile system for the study of immunoassay procedures. The performance of the automated system was monitored by using the turbidimetric detection of barium sulphate …
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Immunoassays with chemically modified bacteriophage
The immunospecific inactivation of bacteriophage is one of the most sensitive methods available for the detection of very low concentrations of antibody. By chemically modifying the phage coat-protein, this sensitivity can be extended to antibodies against a wide variety of haptens and proteins. …
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Evaluation of two SARS-CoV-2 immunoassays
… and anti-spike (quantitative) SARS-CoV-2 immunoassays to determine whether the performance of the assays is acceptable for diagnostic use in the Groote Schuur Hospital virology/chemistry laboratory, as well as other National Health Laboratory Service (NHLS) laboratories in South Africa. …
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Development of diagnostic immunoassays for tularemia and leptospirosis
… were produced for inclusion in diagnostic immunoassays. Mice were immunized with purified F. tularensis lipopolysaccharide and ten monoclonal antibodies were isolated and confirmed to be reactive to pathogenic strains of the bacteria. The antibodies were screened, and reactive pairs …
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Bead-basierte Fluoreszenz-Immunoassays zum Nachweis tumorinduzierter Proteine
In 2002 lung cancer was diagnosed in over 45 000 persons, 39 000 fatalities were registered. According to these statistics, lung cancer prognosis is one of the poorest. Main reason for is the late detection due to diagnosis via optical methods by which a tumor cannot be found till 300 to 400 days …
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Silica based immunoassays for a covalently attached antigen
… silica, but the maximum amount bound was lower. Immunoassays performed using a second enzyme-labelled antibody to detect the monoclonal antibody bound to the silica permitted detection of the antigen at 10-8 M using the modified beads and at10-7 M using the slides.
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Increased sensitivity for lateral flow immunoassays through signal amplification methods
… flow tests (LFTs, also known as lateral flow immunoassays, LFIAs, and rapid diagnostic tests, RDTs) are one of the general approaches for detection of disease targets (e.g. nucleoprotein of the influenza virus). However, the use of these LFTs is limited because of their well-recognized lack of …
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Analysis of phenolic compounds by dint of GDH-biosensors and immunoassays
… von Klärwerken, gezeigt. 2.) Kombination mit Immunoassays Der Einsatzbereich der GDH-Biosensoren lässt sich durch die Kombination mit anderen Techniken erweitern, wobei der Sensor zur Visualisierung der Nachweisreaktion dient. In diesem Fall kann der Sensor zur Bestimmung der Enzymaktivität …
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Designing antibodies to defined epitopes: Immunoassays as structural and analytical probes
Dissertation (Ph.D.)--University of Kansas, Chemistry, 1995.
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Immuntechnologische Verfahren zum Aufbau homogener Immunoassays sowie zur Selektion Antikörper produzierender Zellen
Homogene Immunoassays sind immunologische Testverfahren, bei deren Durchführung vollständig auf Separations- und Waschschritte verzichtet werden kann. Der Substrate Channeling Immunoassay beruht auf der Weitergabe eines Substrates in einem immunologischen Komplex aus zwei Enzymen. Das Produkt des …
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Feline Parasitism: Parasite Prevalence and Evaluation of New Immunoassays for Giardia and Cryptosporidium
… these protozoan parasites can be missed. Fecal immunoassays detect antigens in feces and can have increased sensitivity when compared to traditional microscopic techniques, but still do not detect every infection. The current reference standard is an immunoassay known as the direct …
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Entwicklung, Optimierung und Validierung eines Immunoassays zur sensitiven Detektion des endokrinen Disruptors 17alpha-Ethinylestradiol
… in der Entwicklung und Optimierung von Enyzm-Immunoassays (ELISAs) zur direkten Bestimmung des Analyten im Konzentrationsbereich von 1 - 10 Nanogramm pro Liter. Es wurden drei neue ELISAs für EE2 entwickelt. Für den ersten Assay (Bio-LC EE2 ELISA) wurde ein biotinyliertes …
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Effect of processing parameters on the detection of animal drug residues in milk
… receptor assay, and CITE Probe and LacTek enzyme immunoassays. The procedures evaluated for sulfonamide detection were an HPLC method described by Long et al., Charm II microbial receptor assay, CITE Probe, LacTek and Signal enzyme immunoassays. The methods evaluated for tetracycline detection …
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Confocal laser scanning microscopy of nanoparticles applied to immunosorbent assays
… advantages in comparison to traditional immunoassays. The key point of a confocal immunosorbent assay is the ability to detect the thin layer of immunoassay in the presence of unbound fluorescent reagents without washing the overlayer. Heterogeneous and homogeneous sandwich immunoassays …
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Immunoassay sensitivity and kinetic enhancement in cell culture media using electrokinetic preconcentration
… often performed by the immunoassay. Macroscale immunoassays such as those performed on the tissue culture plate, however, are of limited use for the microfluidic cell culture due to the limited volume of sample generated by the microfluidic culture. The ability to precisely patterning cells on …
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Multiplexed protein biomarker analysis on silicon photonic microring resonators: translation towards clinical diagnostics
… research: detection of biomarkers through immunoassays development, and translation of optimized assays to clinical samples analysis. For the immunoassays development aspect, assays described in this thesis were developed on a platform based on silicon photonic microring resonator …
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