Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Immobilized proteins."”.
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Effects of Immobilization Matrices and Protein Orientation on Biomolecular Recognition at Solid-Liquid Interfaces
… of protein orientation on the forces between immobilized proteins and soluble ligands. Oriented cytochrome b5 monolayers were constructed on supported lipid membranes using a site-selective immobilization method. The orientation of the immobilized cytochrome b5 was well controlled as verified …
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Effects of Immobilization Matrices and Protein Orientation on Biomolecular Recognition at Solid-Liquid Interfaces
… the immobilization of biomolecules, such as proteins, often results in the loss of their biological activities. Factors that impact the apparent kinetics and affinities of the biomolecular interactions at the solid-liquid interfaces were examined with a combination of several physical …
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Design of an in vitro assay to optimize assembly of nanoparticle-tagged nuclear import complexes
… to assess complex formation between nanoparticle-immobilized proteins and soluble binding partners. Two chaperone proteins, importin-a and importin-3 mediate classical nuclear transport, an essential and highly conserved example of protein complex formation in eukaryotic cells. Together, these two …
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Digital Protein Analysis: Technologies for Protein Diagnostics and Proteomics through Single-Molecule Detection
… methods due to intrinsic properties of proteins as well as the complexity of biological fluids, such as blood. This thesis describes the design, development, and testing of several technologies for the advancement of protein detection in clinical and research settings. A common thread …
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Development of a direct photochemical method for the generation of biomolecular interfaces for applications in leukocyte biology
Methods for generating surface-immobilized biomolecular patterns and gradients have provided invaluable insight into the molecular basis of numerous complex biological processes. Herein, we describe the development of a general, photochemical method for creating biointerface substrates presenting …
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Fabrication of nanostructured surfaces with well-defined chemistry using particle lithography
… A were produced with particle lithography. The immobilized proteins remain attached to the surface and form nanopatterns over micron areas corresponding to the thickness of a single layer of proteins. The morphology and diameter of the protein nanostructures are tunable by selecting the ratios …
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Electric field-assisted immobilization and alignment of biomolecules
… could be studied. Permanent immobilization of proteins was examined and quantified absolutely using an atomic force microscope. By measuring the volumes of the immobilized protein deposits, a maximal number of proteins contained therein was calculated. This was possible since the proteins …
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Cytochrome c-DNA and cytochrome c-enzyme interactions for the construction of analytical signal chains
… a biocompatible matrix for the entrapment of proteins. In this study the characteristics of the cyt c and DNA interaction were defined on the molecular level for the first time and the binding sites of DNA on cyt c were identified. Persistent cyt c/DNA complexes were formed in solution under …
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Vibrational spectroscopic studies on [NiFe] hydrogenases: insights into structure and function
… in detail in solution and subsequently also immobilized on surfaces using surface-enhanced infrared spectroscopy to understand its supramolecular arrangement, stability and (redox) reactivity. Experimental results were complemented by theoretical calculations by the group of Prof. Mroginski …
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Mineralized Bionanoparticle Pickering Emulsions
… emulsions were investigated in this work. Proteins (HSA, BSA, fetuin-A), protein cages (ferritin) and plant viruses (CPMV, TYMV, TMV) were employed to produce functional nanopatterned surfaces and interfaces by means of Pickering emulsions which were employed in biomimetic hydroxy apatite …
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FTIR-ATR Characterization of Hydrogel, Polymer Films, Protein Immobilization and Benzotriazole Adsorption on Copper Surface
Plasma polymerization techniques were used to synthesize and deposit hydrogel on silicon (Si) substrate. Hydrogel is a network of polymer chains that are water-insoluble and has a high degree of flexibility. The various fields of applications of hydrogel include drug release, biosensors and tissue …