Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Imaging Mass Spectrometry."”.
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Creating a Map for Surgeons: Direct Blotting Assisted Stamping of Tissue for MALDI Imaging Mass Spectrometry
MALDI imaging mass spectrometry is an attractive technique for the discovery of disease biomarkers from surgically removed tissue sections. It can provide spatial resolution and relative abundance of a wide range of biomolecules across the tissue surface. While the technology has advanced …
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Visualizing the Spatial Localization of Active Matrix Metalloproteinases (MMPs) using MALDI Imaging MS
… ex vivo tissue sample was determined using MALDI imaging mass spectrometry. The MALDI imaging scheme employed a synthetic FRET substrate specific for MMP-2 and MMP-9. The intensity of the m/z value corresponding to the product of active MMP-2 and MMP-9 digestion in each spectrum was extracted to …
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Using imaging secondary ion mass spectrometry to determine mammalian plasma membrane component distribution and cell differentiation state
… until the recent application of high resolution imaging mass spectrometry to biological samples. Using a high resolution imaging secondary ion mass spectrometry instrument (NanoSIMS 50, Cameca), we have chemically mapped the distributions of labeled sphingolipids and cholesterol within the plasma …
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Distinguishing Macrophage Activation States by Mass Spectrometry
… sections. The aim of this work was to find mass spectral biomarkers that allow the differentiation of activation states, and establish conditions that can be used in imaging mass spectrometry (IMS) experiments to investigate the spatial distribution of differently-activated macrophages …
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Sessile Droplet Microfluidic Platforms for Improvements in Mass Spectrometry Sample Preparation
… and detection sensitivity for various mass spectrometry-based analytical techniques. Preliminary results show a significant increase (18-fold) in colourimetric detection sensitivity of cadmium-dye complexed droplets on SETs. SET designs were then modified to improve suitability of the …
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Probing the formation of mammalian cell lipid microdomains with nanometer-scale imaging secondary ion mass spectrometry
… organization. The recent application of imaging mass spectrometry to biological samples has enabled for the chemical analysis of the plasma membrane. Through the use of a high resolution imaging secondary ion mass spectrometer (NanoSIMS 50, Cameca), we have been able to chemically map the …
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Design and implementation of a custom imaging mass spectrometer.
Mass spectrometry (MS) has been demonstrated as one of the most robust and capable analytical tools for chemical analysis. As such, MS has found application across practically every field of science today. One notable area where MS is used today is in the analysis of biological samples, such as …
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Regulation of the FGF14:Nav Interactome by a GSK-3 Centered Pathway
… biochemical assays, high-content confocal imaging, mass spectrometry, and patch-clamp electrophysiology, we show that alteration of this focused GSK-3 interactome, comprised of Akt, Wee1, and CK2, disrupts Nav complex assembly, changes the phosphorylation status of the FGF14 N-terminus, …
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MALDI Mass Spectrometry Imaging for the Discovery of Prostate Carcinoma Biomarkers
… of proteins in tissue sections using MALDI imaging mass spectrometry (MALDI-IMS) has the power to link molecular detail to morphological and pathological changes, enhancing the ability to identify candidates for new specific biomarkers. However, critical questions remain regarding the …
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Biomarker identification in HIV and non-HIV related lymphomas
… biomarkers for DLBCL were determined using MALDI imaging mass spectrometry (IMS) and characterised using LCMS. The expression of one of the biomarkers, heat shock protein (Hsp) 70, was confirmed on a separate cohort of samples using immunohistochemistry. Our results indicate that the …
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Methods and algorithms for quantitative analysis of metallomic images to assess traumatic brain injury
… methods for a novel application of metallomic imaging mass spectrometry (MIMS) to biological tissue. Unlike traditional modalities used to assess neuropathology, that suffer from limited sensitivity and analytical capacity, MIMS uses a mass spectrometer -- an analytical instrument for measuring …
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Developing and utilizing a robust mass spectrometry-based approach to study glucose metabolism and transport in the lens
… Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) analysis of glucose uptake and metabolism in ocular lenses. Specifically, the matrix choice, tissue preparation steps and normalisation strategies were carefully optimised to glucose and its metabolites using …
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Clonal proliferation of hepatocytes during chronic hepatitis B virus infection.
… for HBV surface antigen (HBsAg) expression and Imaging Mass Spectrometry (IMS) for cellular protein expression were carried out to detect cellular changes that may be associated with clonal proliferation. Significantly (p<0.01) larger clones were observed by invPCR in liver DNA extracts of …
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Picosecond Infrared Lasers (PIRL): Applications in Biodiagnostics and Towards Quantitative Mass Spectrometry
… exploited for a new form of spatially resolved mass spectrometry with the prospect of near instant biodiagnostics with mass spectroscopic sensitivity. The ion source for imaging mass spectrometer was based on PIRL ablation with an electrospray pick up source to entrain the ablated species. This …
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A framework for the statistical analysis of mass spectrometry imaging experiments
<p>Mass spectrometry (MS) imaging is a powerful investigation technique for a wide range of biological applications such as molecular histology of tissue, whole body sections, and bacterial films , and biomedical applications such as cancer diagnosis. MS imaging visualizes the spatial distribution …
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Discovery of prognostic biomarkers from the prostate cancer membrane proteome using bioorthogonal chemistry
The link between aberrant glycosylation and cancer progression has formed the basis for detection of cell surface glycans and glycoproteins in the clinical management of cancer. However, the utility of biomarkers has been challenged in recent years due to low cancer specificity and high …
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Secondary ion mass spectrometry imaging of surface-bound microbial communities
… and application of an existing chemical imaging technique, secondary ion mass spectrometry (SIMS) imaging, to study the chemistry underlying surface-bound microbial communities. The first chapter provides a general overview of the dissertation. The second chapter provides an introduction …