Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Imaging Mass Cytometry"”.
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Organotellurium Reagents for Multiparametric Interrogation of Dynamic Biology
… members of a population. The recent emergence of mass cytometry (MC) has offered a new solution to this problem: the ability to simultaneously measure a large number of parameters on a single cell. Unlike traditional fluorescence-based flow cytometry, which suffers from spectral overlap of …
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Chemokine Ligand-3 (CCL3) as a Novel Mediator of Inflammatory Bowel Disease Activity
… IBD were immunostained for CCL3, CCR1, and CCR5. Imaging mass cytometry (IMC) was performed to identify CCL3 cell type expression and CCL3-expressing cell spatial relationships. Immunohistochemistry found that there was no correlation between the severity of inflammation and percent of CCL3 …
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Genomic aberrations as determinants of immune infiltrates in high grade serous ovarian carcinoma
… markers analysed using gene expression data and imaging mass cytometry will also be discussed.
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The Role of Ifitm3 In The Immune Response of Brca-Deficient High Grade Serous Ovarian Carcinoma
… using multiplex immunofluorescence (IF) and imaging mass cytometry (IMC) on our cohort, and HLA mRNA expression was evaluated in HGSOC cell lines. IFITM3 expression was manipulated in HGSOC cells using siRNAs to determine the effect of IFITM3 on the expression of immune-related genes and …
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Investigation of the synovial tissue-homing mechanisms of circulating mononuclear phagocytes in treatment-naïve psoriatic arthritis.
… MPs subsets. I used this to create a suspension mass cytometry panel to further explore 25-different tissue homing markers on blood MPs from healthy, PsA, and cutaneous psoriasis without arthritis (PsC) donors. I discovered a subset of cMos enriched for dendritic cell specific transmembrane …
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Mapping the spatial organisation of B cells and immune signalling in TNBC to characterise response to immunotherapy
… detection of protein and RNA in situ using imaging mass cytometry. We applied this assay to a randomised immunotherapy clinical trial, NeoTRIP, in which patients with TNBC were treated with atezolizumab. Samples were collected from 268 patients at three timepoints: baseline, on-treatment, …
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Analysing the relationship between immune infiltration and tissue architecture in high grade serous ovarian cancer
… Collagen structure was assessed via dual IF/SHG imaging. Structural metrics were correlated with collagen Energy, Correlation and Coherency features. There was no correlation between these collagen features and immune infiltration. Imaging Mass Cytometry was carried out on the ICON7 cohort …
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Tumour ecology of breast cancer patient-derived xenografts
… (scRNA/DNA-seq), three-dimensional imaging with serial two-photon tomography (STPT) and two-dimensional transcriptomic and proteomic profiling (the latter using imaging mass cytometry - IMC). In this thesis, we will present part of this work, focusing in particular on the phenotypic …
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Elucidating intratumour heterogeneity through investigation of clonal, transcriptomic, and proteomic features across models of triple negative breast cancer and diffuse intrinsic pontine glioma
… RNA sequencing based approaches, proteomics, and imaging techniques. The previously developed WILD-seq lineage tracing technology is applied to the D2A1-m2 model of breast cancer metastasis, enabling the tracing of barcoded clones through RNA sequencing. With this, the utility of this technology …
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Graph Representation Learning to Study the Tumour Microenvironment
The progression and treatment response of cancer is influenced by the intricate tissue structure where cancer cells are embedded, known as the tumour microenvironment (TME). Recent technical advances allow the acquisition of highly multiplexed biomedical images (HMBI) that generate spatial tissue …