Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Imaginal Discs"”.
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Genetic analysis of Polycomb group interactions in imaginal discs of Drosophila Melanogaster and transposon tagging of the Asx Gene
… gene to examine the interactions which exist in imaginal discs. The results support several hypotheses: extra sexcombs (esc) and Pc act through different pathways to regulate the homeotic genes; polyhomeotic (ph), Pc, Sexcombs extra (Sce), Sex combs on mid leg (Scm), and Posteriorsex combs (Psc) …
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Optimization of Cytogenetic and Physical mapping of Culicinae genomes
… the analysis difficult. Mitotic chromosomes from imaginal discs of 4th instar larvae of Aedes aegypti were tested as a source for the physical genome mapping for this mosquito. Chromosomes in imaginal discs are 10 times more abundant than chromosomes in nervous ganglia, and they do not accumulate …
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Regulation of growth, patterning and cell fate-specification during imaginal disc regeneration in drosophila
… fate plasticity are regulated in regenerating imaginal discs. Regenerative growth must be carefully controlled and constrained to prevent overgrowth and to allow correct organization of the regenerating tissue. However, the factors that restrict regenerative growth have not been identified. In …
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Functional and developmental characterisation of matrix binding sites in decapentaplegic
… expression of the variants in the wing imaginal disc caused overproliferation of the disc and expansion of the omb target gene expression. The extent of phenotypes correlated with the matrix binding ability of the variants. Corresponding disturbances of the wing vein pattern was observed …
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Myc influences glutamine metabolism to induce autophagy in tumorigenesis
… in the epithelial cells from Drosophila imaginal discs, where different levels of Myc induce cell competition. This process is a mechanism for selection of cells expressing higher level of Myc that acquire a super-competitor condition, with the ability to non-autonomously kill the …
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The role of cellular memory modules in the determination and transdetermination of Drosophila melanogaster imaginal disc cells
… expression of genes involved in patterning imaginal discs during larval development, which expression pattern may be modulated with time. The results demonstrate that expression of one of these genes, hedgehog, once activated, is maintained by a CMM even when the initial activator has …
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Functional Analysis Of A Highly Conserved Cyclin, Cyclin Y, In Drosophila Melanogaster
… defects. Knockdown of CycY but not arrow in imaginal discs resulted in bent legs and blistered wings, suggesting defects in the cell adhesion pathway. A transgene expressing a Cyclin Y variant that is not membrane localized suppresses some of the CycY knockdown phenotypes, further suggesting …
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Functional Characterization of a Cathepsin L in Drosophila Melanogaster
… that invasively grows toward and into the wing imaginal disc. The unfolding of Drosophila wing is a process following eclosion with a cuticular bilayer replacing epithelial cells originally packing the wing. We reasoned that protease functions might be needed for the invasion of ASP into the …
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Multiple axes of inter-organ signaling regulate organ scaling
… signaling, null mutants of babo result in small imaginal discs and small overall body size. This is in part due to metabolic defects caused by defects in Daw signaling. In this dissertation I report how Activin-β and Myoglianin regulate body size and organ growth respectively. In Chapter one, I …
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Notopleural Mutations Enhance Defects In Imaginal Disc Epithelial Morphogenesis And Macrochete Elongation Associated With Mutations in the Stubble-Stubbloid Locus
… actin cytoskeleton dynamics in developing imaginal discs through interactions with the Rho1 pathway. This work will discuss a genetic screen conducted to further investigate the role of Stubble in bristle and imaginal disc morphogenesis. From 50,000 EMS-mutagenized chromosomes 12 enhancers …
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A cytogenetic map for the genomic studies of the West Nile Virus vector Culex tarsalis
… mosquitoes. Using mitotic chromosomes from imaginal discs of 4th instar larvae of Cx. tarsalis, we developed idiograms based on morphology and proportions of the mitotic chromosomes. In addition, the physical mapping of ribosomal genes using fluorescence in situ hybridization was performed. …
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The methuselah family of g protein coupled receptors
… four (Mthl3, 4, 6 and 8) in the larval CNS and imaginal discs and two (Mthl10 and Mth) in both embryos and larvae. We further show Mthl5 expression in the cardiac mesoderm of stage 11 embryos and later restricted to cardioblast cells of the aorta. Loss of mthl5 decreases pericardial-cardial cell …
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Opposing Crosstalk between Rho1 and Cdc42 in Epithelial Morphogenesis
… with Rho1 and Cdc42 during pupal eye and larval imaginal disc morphogenesis. First, clonal analysis in the post-mitotic Drosophila pupal eye epithelium demonstrated that Rho1 was required to maintain AJ integrity independent of its role in sustaining apical cell tension. Rho1 depletion disrupted …
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Cell Polarity Regulates Organ Growth Through The Hippo Pathway
… Crumbs affects growth in <em>Drosophila</em> imaginal discs. I found that <em>crumbs</em> gain and loss of function cause overgrowth and induction of Hippo target genes. In addition, Crumbs is required for the proper localization of Expanded, an upstream component of the Hippo pathway. …
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Function of the forkhead gene fd3F in Drosophila chordotonal neuron differentiation
… in Drosophila embryos and Ch SOPs in larval imaginal discs. I have also generated an fd3F deletion mutant by imprecise excision of a P element. Mutant adults and larvae exhibit impaired coordination characteristic of Ch neuron defects and a similar phenotype was observed in fd3F RNAi lines. …
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Identification of a Novel Regulator of ER Stress-Induced Apoptosis
… easily studied in the homozygous condition. Eye imaginal discs, dissected from the progeny of selected p-element lethal FRT allele males mated to females with GFP-marked wild-type chromosomes were cultured in ER stress inducing conditions and analyzed for differential cleaved caspase 3 activity. …
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Genetic Approaches to Study Tissue Morphogenesis in Drosophila
… localizes in the adherens junction in imaginal disc cells, whereas Sqh2P locates to the apical domain. Sqh1P and Sqh2P also show distinct patterns of expression in embryos. Sqh1P is expressed nearly ubiquitously and outlines cells consistent with a junctional localization, whereas Sqh2P …
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Chromosome and Genome Evolution in Culicinae Mosquitoes
… scaffolds to the mitotic chromosomes from imaginal discs. This study resulted in the construction of a first physical map of the Ae. albopictus genome as well as mapping viral integration and polyphenol oxidase genes. Moreover, comparing our present Ae. albopictus physical map to the …
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The function of chromatin regulators during patterning and growth in Drosophila wing disc regeneration
… of tissue regeneration, focusing on Drosophila imaginal disc regeneration and the known functions of chromatin regulators during tissue regeneration in diverse model organisms. In Chapter II, I describe a genetic screen for chromatin regulators that are required for epithelial tissue …