Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"Illumina Sequencing"”.
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The role of Cladophora glomerata as a habitat for bacterial communities and a reservoir of antibiotic resistance.
… exudates promote the growth of E. coli. Illumina sequencing of the bacterial community indicated that C. glomerata harbours a bacterial community distinct from the surrounding water, and supports the growth of fecal bacteria and bacterial taxa including the genera Clostridium and …
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Analogue enrichment of aromatic degradation through application of lignin-derived phenols
… on DNA stable isotope probing, coupled with Illumina sequencing of labelled DNA, and bioinformatics analysis (Kaiju, Hmmer and Krona). Naturally occurring lignin-derived phenols (vanillin) are found to have influence on the phylogenetic and functional genes of the diverse bacterial population …
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Bacterial and archaeal community structure across a gradient of saline lakes in Kiritimati, Republic of Kiribati
… saline lake microbial communities. Results from Illumina sequencing of 16S rRNA gene amplicons support this finding and also pointed to both salinity and pH as major drivers of community variability. Alpha diversity measurements show no apparent link between salinity and microbial diversity. …
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Examination of mammalian microRNAs by high-throughput sequencing
… gene regulation. Advances in high-throughput sequencing technology have led to discoveries of novel small RNA genes and identifications of their properties. We describe a method for construction of small-RNA library for Illumina sequencing platform that improves upon previous efforts. …
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Functional diversity of fungal symbiont communities and the impact of biotic and abiotic factors on their composition and assembly
… (MAP) and over a span of two years. I used Illumina sequencing of the fungal barcode rDNA (ITS2) to characterize the diversity and composition of fungal communities and employed a community phylogenetic framework to infer the relative contributions of deterministic and stochastic assembly …
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Characterization of the transcriptome and the peptidase and the non-peptidase homologs of callosobruchus maculatus
… and non-peptidase homologs for C. maculatus. Illumina sequencing from the same sample produced 295,690,882 reads which were mapped back to the assembly using BWA software. While C. maculatus does not appear to have an increased number of cysteine peptidases compared with other insects it does …
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Development of Genomic Resources for the Evaluation of Red Snapper, an Emerging Species Candidate for Marine Aquaculture and Stock Enhancement
… population structure using high-throughput sequencing technologies. Eighty-four new microsatellite markers were developed through screening of Illumina paired-end sequencing reads. Microsatellite loci and Single Nucleotide Polymorphisms (SNPs) generated through Restriction Site Associated …
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Effect of Crude Oil on Light Attenuation and the Resulting Impacts on Marine Microbial Phototrophs and Heterotrophs
… rRNA and psbA genes was also performed. Miseq Illumina sequencing analysis was performed to measure the abundance of bacteria and phototrophs. A qPCR was also performed to quantify phototrophs and HCB and UPLC analysis was done to quantify phototroph`s pigments. With continuous light, …
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Molecular ecology of marine isoprene degradation
… a range of marine sites were characterised and Illumina sequencing was used to mine the genomes of isoprene-degrading strains related to Gordonia polyisoprenivorans and Mycobacterium hodleri, isolated from the Colne Estuary, Essex. From these genomes, we retrieved novel sequences encoding …
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Mammalian gene regulation through the 3' UTR
… on poly(A) site usage. Using recent, public, Illumina sequencing data from human nucleosome boundaries, we found evidence that greater nucleosome density in regions flanking but not overlapping poly(A) sites is associated with more frequent usage.
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Ribosomal protein mutants and their effects on plant growth and development
… was non-mitochondrial and probably cytosolic. Illumina sequencing of two r-protein mutant transcriptomes, pfl1 (rps18a) and pfl2 (rps13a), identified a novel set of up and down regulated genes, previously unknown or linked to r-protein mutants. The 20 genes identified were classified into four …
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The effects of warming on carbon and microbial community wetland dynamics at Turnbull National Wildlife Refuge, Washington
… and relative abundance was determined using Illumina sequencing protocol for the amplification of the V4-V5 region of the 16S rRNA gene. Results showed that both abundance and diversity decreased with warming and depth within permanent wetlands, although no variation in species composition …
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Analysis of the effect of genetic heterogeneity on de novo genome assembly using Xylocopa virginica as a model.
Next generation sequencing (NGS) technology has revolutionized genomic and genetic research, and as a result, de novo genome sequencing and assembly for non-model organisms has now become a common task in genome research. However, the integral properties of a genome such as ploidy, mutations, and …
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Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing
… particular, advances in massively parallel DNA sequencing and synthesis technologies are defining our genomes and the products they encode. In the first part of this thesis, we have constructed a rationally designed antibody library and analysis platform optimized for use with deep sequencing …
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Next Generation Sequencing and Genome-Wide Association Studies to Identify Mitochondrial Genomic Features Associated with Diabetic Kidney Disease
… DKD related phenotypes.<br/><br/>Next generation sequencing offers the ability to investigate genetic and epigenetic features in more detail than genetic association studies and these have been successful used for research into various disease including DKD. In Chapter 3 of this thesis Illumina …
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The Effect of Environmental Perturbations on the Plant Phyllosphere Microbiome
… communities were characterized through targeted Illumina sequencing of the 16S rRNA taxonomic marker gene. Biogeographic patterns of bacteria in the phyllosphere were found to be evident from southern magnolia (Magnolia grandiflora) trees 1-452 m apart in a small forest plot. A significant …
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Diversity, characteristics and potential for biological control of Sclerotinia sclerotiorum mycoviruses in New Zealand
… of which had a hypovirulent phenotype. Data from Illumina sequencing showed that mycoviruses in S. sclerotiorum are more diverse than was apparent from dsRNA profiles. Twelve viral genomes were fully sequenced most of which were novel, increasing the total number of S. sclerotiorum mycoviruses …
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Exploring the evolution of social behaviour using genomic data
… expressed genes. To this end, we used 454 GS FLX sequencing technology to generate the brain Expressed Sequence Tags (ESTs) of twelve socially diverse bees. The ESTs were assembled into species-specific non-redundant contigs and singletons, which were loaded into a MySQL database using custom …
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Identification and Characterization of Y Chromosome and M Locus Genes in Anopheles and Aedes Mosquitoes Using the Chromosome Quotient Method
… from separate pools of female and male Illumina sequencing data. The focus of my dissertation is using the CQ method to identify potential male-determining factors in Aedes and Anopheles mosquitoes. First, we identified a novel gene tightly-linked to the M locus in Aedes aegypti called …
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A mosquito-specific bZIP transcription factor and the influence of a Y-specific gene on sex determination in Anopheles stephensi
… embryonic miRNAs as determined by small RNA sequencing and Northern analysis. Promoter activity for bZIP1 was characterized and the promoter was used to direct maternal and zygotic transgene expression in An. stephensi. Y chromosome genes were successfully identified in An. stephensi from …
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