Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"IgG2"”.
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Mechanistic approaches towards understanding particle formation in biopharmaceutical formations. The role of sufactant type and level on protein conformational stability, as assessed by calorimetry, and on protein size stability as assessed by dynamic light scattering, micro flow imaging and HIAC
… Light Scattering (DLS), HIAC and MFI. BSA and IgG2 were used as model proteins. Thermal unfolding experiments indicated a very weak surfactant-immunoglobulin IgG2 interaction, compared to much stronger interactions for the BSA surfactant systems. The DLS results showed that BSA and IgG2 with …
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IgG subclasses, specific antibodies and immunoglobulin allotypes in children with invasive Haemophilus influenzae type B and Staphylococcus aureus infections
… ELISAs for measuring the IgG subclasses (lgGl, IgG2, IgG3 and IgG4) and subclass antibodies specific to Haemophilus influenzae polyribosylribitol phosphate, Staphylococcus aureus teichoic acid and tetanus toxoid. The relative affinity of antibodies in these ELISAs was determined by the …
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Immunological evaluation of DNA vaccines against Haemonchus contortus and Toxoplasma gondii in sheep
… higher levels of ES-specific IgA and IgG2 were found in this group. In the group treated with pSecTag2-15, FEC was negatively correlated with IgG2 level to adult H. contortus, after challenge. The pSecTag2-24 DNA vaccine did not contribute to protection. The efficacy of the DNA …
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Resposta imune de bovinos vacinados com peptídeo sintético SBm7462 com vistas ao controle do Boophilus microplus (Canestrini, 1887)
… realizados a identificação de isotipos IgG1 e IgG2 antígeno-específicos, determinação do perfil de linfócitos circulantes (CD4+, CD8+, CD21+ e WC1+) por citometria de fluxo e seguimento dos eventos microscópicos ocorridos em linfonodos através de técnicas diferenciais e de imunohistoquímica. A …
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Localization of FC Receptors on Murine Spermatozoa.
… the mouse, while Johnson (1968) showed it to be IgG2 in the guinea pig. IgM and IgA do not show detectable binding to mammalian spermatozoa (Allen and Bourne, 1978).
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Characteristics of Donor-Specific anti-HLA Antibodies (DSA) Impacting different Renal Allograft Outcomes
… IgG subclass (49.4%), followed by IgG3 (24.7%), IgG2 (16%), and IgG4 (9.9%). Among DSA characteristics, DSA MFI and IgG1 subclass were strongly correlated with C1q positivity (p= 0.01 and p= 0.009, respectively). Further studies are needed to investigate the clinical relevance of high MFI DSA and …
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Allergic bronchopulmonary aspergillosis in cystic fibrosis: prevalence, clinical complications, diagnosis and influence on the outcome
… hyphal (WSSH) antigen with increase IgG, IgG1, IgG2 and IgG4 antibodies levels in patents with ABPA. Patients with asthma and 4 Nelson criteria may have a similar pattern of specific IgG and subclass antibodies. On studying correlation between the specific IgG and subclass antibodies , …
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Heterogeneity of injury in vasculitis: influence of anti neutrophil cytoplasm antibody IgG subclass and endothelial susceptibility.
… Neutrophils were captured by normal IgG3>IgG1>IgG2/IgG4. Blockade of CD32 affected IgG3, CD16, IgG1/2. Neutrophils exposed to soluble ANCA IgG1/3 adhered to cytokine-activated endothelial cells, as did IgG4, not previously thought to bind constitutively expressed CD16/CD32. Fc blockade reduced …
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Aggregation of IgG mAb Biotherapeutics: Sources, Methods of Characterization, and Biological Implications
… in solution and one in lyophilized form and one IgG2 mAb in solution) were stressed by a variety of different conditions and the resulting aggregates and particles were characterized using a broad array of methods. Some of the characteristics examined included aggregate/ particle size, count, and …
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Analysis of the molecular interplay between Streptococcus pyogenes and its human host
… specifically cleaving the hinge region of IgG1, IgG2, IgG3, and IgG4. Moreover, data suggesting that neutrophil proteinases release immunogenic epitopes from IdeS are presented. This is a novel mechanism by which S. pyogenes exploits the human immune system and prevents its virulence factors from …
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Untersuchungen zur antiangiogenen Aktivität des matrizellulären Proteins Thrombospondin-2
… hergestellt, das als Fusionsprotein an IgG2-Fc gekoppelt ist. Die Behandlung von mikrovaskulären Endothelzellen (HDMEC) mit NTF-TSP-2 führte zu einer deutlichen Hemmung der VEGF-induzierten Ausbildung von Endothelzellröhren. Durch die systemische Gabe von NTF-TSP-2 wurden das …
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Increases in Cortisol due to Weaning Stress and the Subsequent Alterations to Immune Function in Beef Calves
… analyzed for IFN? and IL-4 as well as IgG1 and IgG2 specific antibodies to RB51. Fecal samples were analyzed for cortisol metabolites. Both IgG1 and IgG2 antibodies to RB51 increased from day 0 to day 14 (P<0.05), however no differences were detected between treatment groups. Fecal cortisol …
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Respuesta inmune celular y humoral del porcino ibérico frente a la metastrongylosis
… esta ultima, se analizaron las subclases IgG1 e IgG2. En lineas generales, los valores de densidad optica obtenidos frente al antigeno larvario durante las tres primeras semanas de la infeccion fueron mas elevados que los mostrados frente al antigeno somatico de adultos. En cambio, a partir del …
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Cellular requirements for antibody production in a novel LPS-enhanced model of autoimmune myasthenia gravis
… amounts of secreted high-affinity anti-AChR IgG2,\r\nimmune complex deposits (IgG, C3, MAC) in muscle, and elevated sera levels of the Bcell survival factor, BAFF. Our results indicate that LPS abrogated B cell differentiation\r\nto antibody secreting cells in the LPS-EAMG model. Furthermore, …
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Evaluation of Highly Conserved Burkholderia pseudomallei Proteins as pan-Burkholderia Vaccine Antigen Candidates
… assays and ELISAs, which identified IgA and IgG1/IgG2-balanced antibody responses paired with robust Th1 and Th17 cellular immunity. We also confirmed the ability of the OmpA1 and OmpA2 vaccines to protect mice from a lethal B. pseudomallei challenge. Finally, we measured the ability of …
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Preparation and characterization of Staphylococcal protein A adsorbents for high performance affinity chromatography
… non-retained serum fractions. Retention of IgG1, IgG2 and some IgA was shown. IgM, some IgA and albumin were not retained. Analysis of reference serum without pretreatment was performed using IgG standards. Good agreement was observed between the IgG concentrations measured by this method and by …
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Characterization of the Capsular Polysaccharide of Haemophilus parasuis and its Application in the Diagnosis and Prevention of Glasser's Disease
… CP-CTB conjugate produced a significantly higher IgG2/Th2 response than unimmunized mice or mice immunized with only CP, and immunized mice had fewer bacteria in their tissues that unimmunized mice. The CP conjugate produced a robust IgG antibody response to CP when used to immunized piglets, but …
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DEVELOPMENT OF A SUBUNIT LAWSONIA INTRACELLULARIS VACCINE
… with Montanide 61 + Antigens induced a stronger IgG2 profile but a more balanced response was observed in the Montanide 660 + Antigens group. Further, when measuring antibody avidity of the Montanide 61 + Antigens sera, GroEL and YopN antibodies had better antibody avidity than FliC antibodies. …
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Early-life immunity and susceptibility to Mycobacteria
… levels of measles-specific IgG and BCG-specific IgG2. No active helminth infections were detected, but the presence of Ascaris lumbricoides- and Trichuris trichiura-specific class-switched antibodies indicated prior exposure. No association was found between helminth-specific humoral responses …
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On-farm strategies for the prevention and detection of Gram-specific clinical mastitis in dairy cows
… of CM, V1 cows produced more serum IgG1 and IgG2 than V2 cows. Our results indicated that the effects of vaccination were diminished in mid-lactation, and that antibodies are not the limiting factor in defending against induced E. coli mastitis. Multiple regression models, incorporating the …
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