Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 103 for “"IgG1"”.
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Atm-/-Ung-/- Mice Present Reduced Levels of Switched Immunoglobulin Isotypes IgG1 and IgG3
… mice produce levels of IgG1 comparable to <em>Atm<sup>-/-</sup></em> mice and IgG3 levels that are negligible, but quantifiable, suggesting that ATM may selectively drive MMR depending on Ig isotype.</p>
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The interactions of tolerogenic dendritic cells, induced regulatory T cells and antigen-specific IgG1-secreting plasma cells in asthma
… with accumulation of eosinophils, IgE and IgG1 production, and airway hyperresponsiveness. We reported previously that treatment of OVA-asthmatic mice with allergen-presenting IL-10-differentiated dendritic cells (DC) (DC10) leads to progressive and long-lasting full-spectrum asthma …
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동물 세포의 세포질에서 발현된 IgG 의 중쇄와 경쇄간의 조합 여부 및 항원결합 활성 분석
… 결합능력 유지 여부가 자세하게 연구된 것이 없다. 따라서 본 연구에서는 키메릭 3D8 IgG1 (chimeric 3D8 IgG1, ch 3D8 IgG1) 발현벡터를 세포질에 발현시켜 중쇄와 경쇄간의 결합 및 항원 결합능력을 조사하였다. 세포질에서 발현되도록 선도 서열 (leader sequence)을 제거하여 중쇄와 경쇄를 동시에 발현하는 벡터를 제조하였다. ch 3D8 IgG1의 기본구조 외에도 중쇄의 경첩 부위 (hinge region) 및 중쇄-경쇄 사이의 cys 잔기를 세린 (Serine, Ser) 잔기로 치환한 3D8 …
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The role of Human Endogenous Retrovirus K10 (HERV-K10) in the pathogenesis of rheumatoid arthritis via molecular mimicry
… of rheumatoid factor (RF) autoantibodies to IgG1 Fc, and anti-citrullinated protein antibodies (ACPA) to citrullinated autoantigens such as fibrinogen. Although RA pathogenesis has not been elucidated, genetic predisposition, environmental insults and viral pathogens are considered …
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Characteristics of Donor-Specific anti-HLA Antibodies (DSA) Impacting different Renal Allograft Outcomes
… MFI values, C1q positivity and IgG subclasses. IgG1 was the predominant IgG subclass (49.4%), followed by IgG3 (24.7%), IgG2 (16%), and IgG4 (9.9%). Among DSA characteristics, DSA MFI and IgG1 subclass were strongly correlated with C1q positivity (p= 0.01 and p= 0.009, respectively). Further …
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Heterogeneity of injury in vasculitis: influence of anti neutrophil cytoplasm antibody IgG subclass and endothelial susceptibility.
… IgG. Neutrophils were captured by normal IgG3>IgG1>IgG2/IgG4. Blockade of CD32 affected IgG3, CD16, IgG1/2. Neutrophils exposed to soluble ANCA IgG1/3 adhered to cytokine-activated endothelial cells, as did IgG4, not previously thought to bind constitutively expressed CD16/CD32. Fc blockade …
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Characterization, Stabilization and Formulation Design of IgG and Secretory IgA Monoclonal Antibody Candidates during Storage and Administration
… In these two chapters, two different human IgG1 molecules (mAb-J and mAb-C), which showed strong RSA propensity at relatively high protein concentrations, were comprehensively studied to better understand their solution properties and molecular behaviors by both biophysical techniques as …
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Exploration of novel linker scaffolds enabling the simultaneous rebridging of disulfide bonds for the synthesis of antibody-drug conjugates
… rebridges all interchain disulfide bonds in an IgG1 antibody, has been developed and was recently reported by the Spring Group. The work reported in this thesis explores the translation of this ‘all-in-one’ rebridging linker technique to the generation of azide-containing antibody-linker …
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Cyst morphology and serological variation in cystic echinococcosis patients from Turkana, Kenya
… Significant differences in expression of IgG1 and IgG4 was found in response to some antigens. In simple cysts (CE1), IgG4 was highly expressed in comparison to IgG1, in response to HSP70 and EgEF-1β/δ while in cases where the characterised by cyst infiltration and calcification (CE4) …
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Behavioral biomarkers for calf health
… total plasma protein; Immunoglobulins, IgG1 and IgM). Variables collected included: each calf’s first-meal suckling behaviors; automated activity behaviors, and measures of passive transfer of maternal antibodies (IgG1, Igm, hematocrit, and total plasma protein). There was a tendency for …
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Engineering novel antibody-based molecules to probe the role of the immunoglobulin hinge and generate a potential cancer vaccine
… distance between antigen binding sites, human IgG1 mutants specific for the hapten NIP which featured different upper hinge lengths were generated. Rigid bivalent ligands or “molecular rulers” (dsNIP-DNA) comprising defined lengths of double stranded DNA with NIP covalently attached to both …
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Development of a Passive Immunization Strategy Against Atherosclerosis
… We tested the effects of recombinant human IgG1 antibodies against two of these peptide sequences on the development of atherosclerosis in mice. Passive immunization with the IEI-E3 and 2D03 antibodies, specific for MDA-p45 (aa 661-680), significantly inhibited atherosclerosis progression …
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Human antibody-mediated immunity against infection and parasite fecundity in Schistosoma mansoni and Schistosoma haematobium infection
… immune response. It is proposed that IgG1 antibodies raised to proteins within whole worm extract are responsible. A proteomic approach used to identify targets of the IgG1 returned 191 proteins, several of which are closely associated with the parasite-host interface. Three were …
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The Role of RNA Helicase RHAU in Immunoglobulin Class Switch Recombination
… B cells show decreased <em>in vitro</em> CSR to IgG1 and <em>Rhau</em><sup>D/D</sup> mice display decreased IgG1, IgA, and IgG3 titers, suggesting that RHAU regulates CSR.</p>
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Engineering the antibody-TRIM21 interface for the improved intracellular clearance of pathogenic targets
… targets. A single Fc mutation in human IgG1, T256P, increases affinity for human TRIM21. X-ray co-crystal structures of the TRIM21 PRYSPRY domain bound to Fc-T256P reveal a conformational change in IgG1 Fc that stabilises interactions between the antibody CH2 domain and TRIM21 PRY …
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Resposta imune de bovinos vacinados com peptídeo sintético SBm7462 com vistas ao controle do Boophilus microplus (Canestrini, 1887)
… foram realizados a identificação de isotipos IgG1 e IgG2 antígeno-específicos, determinação do perfil de linfócitos circulantes (CD4+, CD8+, CD21+ e WC1+) por citometria de fluxo e seguimento dos eventos microscópicos ocorridos em linfonodos através de técnicas diferenciais e de …
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Applicability of Using Physical Stability Data and Advanced Visualization Methods in Protein Comparability Studies
… minor (different glycosylation patterns of an IgG1 mAb and Fc molecules) structural differences between proteins. In addition, for the IgG1 Fc glycoforms expressed in yeast, the effect of N-linked glycosylation site occupancy, as well as the effect of different charge states in various …
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The influence of maternal Nippostrongylus brasiliensis infection on immunity in offspring
… in the transfer of Nb antigen (NES)-specific IgG1 in utero and through breastmilk, changes in lymphocyte populations and early germinal center formation in naive offspring. Maternal Nb infection does not interfere with control of STm in offspring in BALB/c mice, but may interfere with control …
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Aggregation of IgG mAb Biotherapeutics: Sources, Methods of Characterization, and Biological Implications
… three different IgG monoclonal antibodies (2 IgG1 mAbs, one in solution and one in lyophilized form and one IgG2 mAb in solution) were stressed by a variety of different conditions and the resulting aggregates and particles were characterized using a broad array of methods. Some of the …
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Estudos de imunobioinformática e imunoprofilático de uma proteína de fusão contra a infecção por Corynebacterium pseudotuberculosis em modelo murino
… and observed for 30 days. Total IgG, IgG1 and IgG2a antibodies were quantified, and a culture of splenocytes from 3 animals from each group was made, with further antigenic stimulation and quantification of the expression of IL-4, IL-12, IL-17, TNF and IFN-γ. The in silico analysis …
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