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Showing 1 to 20 of 34 for “"Identification of proteins"”.
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Identification of Proteins Involved in Chloroplast DNA Replication
… Gel spots were excised and analyzed by MALDI-ToF mass spectrometry, resulting in several protein identifications. The proteins identified all have functions unrelated to DNA replication. While some of these proteins may be due to contamination, it is possible that some of these proteins are …
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Identification of proteins interacting with lupin and Arabidopsis tRNA nucleotidyltransferases
… nucleotidyltransferase catalyzes the addition of CMP and AMP residues to the 3 ' ends of tRNAs that have an incomplete CCA sequence. In eukaryotic cells, nuclear, mitochondrial and chloroplast genomes all encode tRNAs. Therefore, although tRNA nucleotidyltransferase is synthesized in the …
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Identification of proteins interacting with the human mismatch repair protein MLH1
Loss of expression of the human DNA mismatch repair (MMR) gene, hMLH1, is seen in a number of tumour cell lines resistant to a variety of cytotoxic drugs. The aim of this study was to identify other proteins that interact with hMLH1 to attempt to further elucidate its role in MMR and the engagement …
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Identification of proteins controlling gastrulation movements by a proteomic approach in zebrafish
… gastrulation, a well-orchestrated series of cell movements leads to the formation of the three germ layers: ectoderm, mesoderm and endoderm. In zebrafish, a model organism for vertebrate development, the mesendodermal progenitor cells separate from the ectodermal cells and migrate towards …
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ELECTROPHORETIC AND ENZYMATIC IDENTIFICATION OF PROTEINS ASSOCIATED WITH THE DEVELOPMENT OF COMPETENCE IN BACILLUS SUBTILIS
… zymogram procedure for the analysis of nucleases is described. Using purified deoxyribonuclease I (bovine pancreas), as little as 10 pg of nuclease can be detected. Isoelectric focusing (IEF) and non-equilibrium pH gradient electrophoresis (NEPHGE) were compared as first dimensions, …
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Improving Soybean Resistance to Cyst Nematodes and Fusaria: Near Isoline and Transgenic Analyses of the Rhg1/Rfs2 Locus and Identification of Proteins That Bind to Receptor Kinases
Soybean is one of the most important grain legumes grown in US and worldwide, and is a major component of human and animal protein diets. Despite improvements in management practices, and the introduction of improved soybean cultivars, soil borne pathogens continue to cause tremendous yield loss in …
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Nephrotic Syndrome and Glomerular Basement Membrane: Genetic Defect of the Laminin α5 Chain
Nephrotic syndrome is a heterogeneous group of disorders characterised by renal and extra-renal manifestations. Classic symptoms of nephrotic syndrome include severe proteinuria, hypoalbumiaemia, oedema and hyperlipidaemia. <br></br><br></br> Genetic studies of hereditary forms of nephrotic …
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Development and Application of Proteomic Technologies for the Analysis of Post-Translational Modifications
… and dynamic means for diversifying the chemistry of the ~20 ribosomally coded amino acids. As such, they provide an ideal mechanism for promoting cellular adaptability by facilitating the tuning of protein interactions and functions in response to changing environmental conditions. Despite their …
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Preliminary characterization of VmTPT2, a putative monoterpene indole alkaloid (MIA) transporter from Vinca minor L. (Apocynaceae)
The various steps of monoterpene indole alkaloid (MIA) biosynthesis are known to occur in specialized cell types and subcellular compartments. Numerous MIAs display powerful biological activities that have led to their use as pharmaceutical treatments for cancer, hypertension and malaria. Many of …
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Archaeal Basal Transcription Factors: DNA and Protein Interactions
… transcription system is a stripped-down model of the complex eucaryal one, having only one RNA polymerase and two known and characterized basal factors: Transcription Factor B (TFB) and TATA-Binding Protein (TBP). After the first sequence analysis of an archaeal genome (Bult et al., 1996), …
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Characterization of Protein Complexes and Protein Interaction Networks by Mass Spectrometry
The major goal of this study was to develop an experimental proteomics approach for deciphering protein complexes and protein interaction networks in the budding and fission yeasts. Key steps of the employed analytical routine, including the purification of complexes and mass spectrometric …
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Systematic C-degron activity profiling implicates conditional regulation of the CTLH E3 ligase complex in ribosome maturation.
… protein termini, yet the physiological relevance of these N-degron and C-degron pathways remains poorly understood due to the limited number of validated substrates. Thus, the major aim of this thesis was to define how C-terminal degrons contribute to the regulation of protein stability in human …
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Enabling tools for biological analysis : technologies for the study of protein dynamics, detection and interaction
The study of proteins in biological systems requires a comprehensive approach: investigating dynamics, interaction and identification. This thesis will examine several technological approaches we have developed to address these needs. To enable the study of the dynamics of biological systems, we …
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Identification of novel components that connect cellulose synthases to the cytoskeleton
… structure in plant cell walls. Cellulose microfibrils are laid down in a tight parallel array, surrounding plant cells like a corset. Orientation of microfibrils determines the direction of growth by directing turgor pressure to points of expansion (Somerville et al., 2004). Hence, cellulose …
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Mapping the cell cycle-dependent centrosomal interactome
A series of highly regulated and precisely ordered events must occur for cells to multiply. Understanding the dynamic interactions of proteins driving this process are crucial for deciphering cell fate. The centrosome is a non-membrane-bound organelle that acts as the primary microtubule organizing …
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Photo-cross-linking and identification of nuclear proteins that bind to DNA containing a site-specific adduct of cis-[Pt(NH₃)(N-6-aminohexyl)-4-benzophenonamide)CI₂]
… has been used for over 30 years in the treatment of several types of malignancies, with the highest success rates in testicular cancer patients. Despite the triumphs of this drug, the exact mechanisms of cell-killing by cisplatin are not fully understood. A more complete understanding of the …
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Proteomic Evaluation and Cytotoxicity of Red Maple (Acer rubrum) Leaves
… rubrum</em>), also known as swamp, water or soft maple, is endemic to eastern and central North America and was widely used as traditional medicine by the first peoples. Commercially, its well-known products include maple syrup and high-quality lumber. The potential medicinal benefits of …
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