Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"ITS2"”.
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Using Molecular Diagnostics Based On Internal Transcribed Spacer 2 Sequence To Study Geographical Distribution of Holarctic Malaria Mosquitoes
… of An. daciae and An. persiensis. Mosquito ITS2 ribosomal DNA was extracted and amplified using polymerase chain reaction (PCR) methods. The PCR products were then sequenced and analyzed based on GenBank information obtained. An analysis by Restriction Fragment Length Polymorphism using ITS2 …
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DNA Barcoding of Ayurvedic Herbal Supplements used for the Purported Treatment of COVID-19
… with four barcoding regions (i.e., rbcL, matK, ITS2, and mini-ITS2) using two composite samples per product. The use of the combined loci resulted in the amplification of 85.2% of the products and identification of at least one species in 64.8% of the products (based on the combined results of …
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Development of Bioassays Based on Nucleic Acid Amplification and Next-Generation Sequencing
… its origins. We targeted the nuclear ribosomal ITS2 region of plant DNA, which is known to support genus-level discrimination. We purified pollen-free DNA from honey, filtered or centrifuged to remove all pollens, using three different methods: (i) anti-dsDNA antibodies coupled to magnetic …
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Use of a Novel Combination of Multiplex PCR and DNA Barcoding in Assessing Authenticity of Ginseng Products
… with three genetic targets (i.e., rbcL, ITS2, and matK). Of the three genetic markers tested with DNA barcoding, ITS2 had the greatest amplification success at 74% and matK had the greatest sequencing success at 60%. DNA barcoding alone was able to identify species in 68% of products, …
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Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… (rbcL) and internal transcribed spacer 2 (ITS2), amplified with universal primer pairs in a two-step polymerase chain reaction (PCR) library preparation for Next Generation Sequencing (NGS) with MiSeq Illumina V2 systems. The sequence reads generated were assessed for quality, …
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Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… (rbcL) and internal transcribed spacer 2 (ITS2), amplified with universal primer pairs in a two-step polymerase chain reaction (PCR) library preparation for Next Generation Sequencing (NGS) with MiSeq Illumina V2 systems. The sequence reads generated were assessed for quality, …
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DNA barcoding of forensically important blow flies (Diptera: Calliphoridae) within the Western Cape of South Africa
… DNA barcodes were generated for the COI and ITS2 markers for 41 forensically relevant Calliphoridae specimens, representing seven species from six localities in the Western Cape: Chrysomya albiceps (n = 3), Chrysomya chloropyga (n = 8), Chrysomya marginalis (n = 5), Chrysomya megacephala (n = …
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DNA barcoding of forensically important flies in the Western Cape
… marker, the second internal transcribed spacer (ITS2) were analysed. Phylogenetic analyses for both barcodes showed high interspecific divergence values which are desirable for species level differentiation by DNA barcoding. COI sequences from adult flies were also submitted and searched against …
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The role of thermal history in shaping the microbiome of Red Sea corals
… Red Sea. Symbiodinium clade type (ITS2) and microbial community (16S rRNA metagenomics) associated with six key coral species persisting across five sites of the northern Red Sea (29°-20°N) were then examined. Symbiodinium clade identity associated with each coral species generally …
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Helping to Resolve Taxonomic Conflicts within the Genus Amblyomma (Acari:Ixodidae) from a Molecular Perspective
… 16SrDNA, DL, COI, COII (mitochondrial) and ITS2 (nuclear), I analyzed the systematic relationships between these taxa and their closest relatives. Phylogenetic analyses by maximum parsimony, maximum likelihood, and Bayesian analysis were performed in order to determine relationships among …
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Microbiome associated with Ulva lacinulata and seawater in an integrated abalone (Haliotis midae)–Ulva system with partial recirculation
… for the 16S rDNA (QIIME2 vs. mothur) and ITS2 (PIPITS vs. QIIME2) genes to evaluate how different bioinformatics pipelines influence taxonomic classification and subsequent diversity analyses. In chapter 3, culture- dependent techniques were used to assess changes in the abundance of …
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Taxonomy and phylogeny of the South African Plocamium species Rhodophyta, Plocamiaceae
… and DNA sequence characters (ITSJ-5.8S-ITS2) in both independent and combined Bayesian and parsimony analyses to investigate the relationships within South African Plocamium species and between South African Plocamium species and those from elsewhere. South African Plocamium forms a …
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Functional diversity of fungal symbiont communities and the impact of biotic and abiotic factors on their composition and assembly
… Illumina sequencing of the fungal barcode rDNA (ITS2) to characterize the diversity and composition of fungal communities and employed a community phylogenetic framework to infer the relative contributions of deterministic and stochastic assembly processes. I found that spatial distance and MAP …
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Interrelationships Among Monorchiid Trematodes with Special Emphasis on Some Northwestern Atlantic Genera
… as molecular data from additional DNA regions (ITS2 rDNA, mtDNA) and data from more geographic locations and intermediate hosts.</p>
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A study of basidiomycetes isolated from coarse woody debris and contiguous soil horizons in a mixed deciduous-conifer forest in New Hampshire, United States of America
… nuclear ribosomal DNA (nrDNA) in the ITS1-5.8S-ITS2 region using the primers ITS1-4.</p>
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Infection of two distinct Trichuris sp. genotypes within and among baboon (Papio ursinus) troops on the Cape Peninsula, South Africa
… different sized products of the ITS1-5.8S-ITS2 region of the ribosomal DNA through PCR. Diagnostic PCR revealed the DG genotype among two Trichuris sp. specimens in a baboon from an unknown troop, while the CP-GOB genotype was observed among five specimens within a baboon from the Groot …
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Investigating the photosynthesis of Symbiodinium in Caribbean gorgonian corals
… Symbiodinium internal transcribed spacer 2 (ITS2) types. P. porosa and P. anceps harbored the same Symbiodinium type but exhibited markedly different photosynthetic characteristics, which highlights the importance of host morphology on Symbiodinium performance. I also compared the …
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Molecular phylogeny and morphological reconstructions of Plagiochilaceae (Jungermanniopsida) with hypotheses on biogeography and divergence times
… Erstellung von Sekundärstrukturen für ITS1 und ITS2 angewandt und bietet damit das erste Strukturmodell für Lebermoose aus diesem Bereich.Diese genetischen Daten wurden mit 34 der Literatur entnommenen morphologischen Daten verglichen, die auf die drei-Marker-Phylogenie geplottet wurden. Die …
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Využití vybraných molekulárních metod k metabolické charakterizaci průmyslově významných kvasinek
… metodami, zahrnující sekvenční analýzu ITS1, ITS2 a D1/2 ribozomálního operonu a analýzu mini a mikrosatelitních sekvencí M13 a GTG5. Druh R. toruloides je známý jako vynikající producent mastných kyselin, a proto se v poslední době stal cílovou karotenogenní kvasinkou pro vývoj nástrojů pro …
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Systematic studies on Thysanotus R.Br. (Asparagales: Laxmanniaceae).
… intron and trnL–F intergenic spacer) and nuclear ITS2 gene regions were amplified and the results compared and combined with a morphological analysis. Phylogenetic analyses were carried out using Arthropodium R.Br and Eustrephus R.Br as outgroup taxa. There was sufficient variation in general …
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