Global ETD Search
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Showing 1 to 13 of 13 for “"ISO-1"”.
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Thermodynamics and Kinetics of Iso-1-cytochrome c Denatured State
… in the denatured state using a P25A yeast iso-1-cytochrome c variant. The findings indicated that intermolecular aggregation in a denatured protein is extremely fast – 107-108 M-1s-1 and that the P25A mutation strongly affects intermolecular aggregation. This work suggests that equilibrium …
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Conformationally gated electron transfer studies of iso-1-cytochrome c
… has also been applied to the dynamics of proline isomerization. The data demonstrate that rates of ET in proteins can be tuned efficiently using a combined strategy of modulating the sequence position and nature of the metal ligand involved in conformational gating. Thus, ET gates can be readily …
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Thermodynamics and Kinetics of Iso-1-cytochrome c Denatured State
… in the denatured state using a P25A yeast iso-1-cytochrome c variant. The findings indicated that intermolecular aggregation in a denatured protein is extremely fast – 107-108 M-1s-1 and that the P25A mutation strongly affects intermolecular aggregation. This work suggests that equilibrium …
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Conformationally gated electron transfer studies of iso-1-cytochrome c
… has also been applied to the dynamics of proline isomerization. The data demonstrate that rates of ET in proteins can be tuned efficiently using a combined strategy of modulating the sequence position and nature of the metal ligand involved in conformational gating. Thus, ET gates can be readily …
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Μελέτη του ρυθμιστικού ρόλου του παράγοντα αναστολής της μετανάστευσης των μακροφάγων (MIF) στην επίδραση των κορτικοειδών στην παραγωγή μεταλλοπρωτεασών και των ενδογενών αναστολέων τους, κυτταροκινών και κολλαγόνου στο ρινικό πολύποδα
… της καταστολής της ενεργοποίησης του NF-κB. Ο ISO-1, ένας αναστολέας της δράσης του MIF, ενίσχυσε σημαντικά την κατασταλτική επίδραση της δεξαμεθαζόνης στα επίπεδα της IL-6 και του TIMP-1 στο μέσο καλλιέργειας ιστού από ΡΠ, ενώ αντίθετα προκάλεσε αναστροφή της κατασταλτικής δράσης της …
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Numerical Modeling for the Solute Uptake from Groundwater by Plants-Plant Uptake Package
… to select a modeling category of three: Linear (ISO-1), Freundlich (ISO-2), and Langmuir (ISO-3). Each modeling option depends on the designer selection according to the fitted equation parameters between, C and, M. In terms of conservative results, ISO-1, and ISO-2 give less mass removal results …
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Rational Design of Chemotherapeutic Agents by Quantitative Structure-Activity Relationships and Solution NMR Methods for High Resolution Protein Structure Refinement
… 13C, 15N-labeled Saccharomyces cerevisiae iso-1 cytochrome c was overexpressed in Escherichia coli and purified in its ferric form for NMR studies. The chemical shifts of most of the 1H, 13C, and 15N nuclei were assigned for future analyses of the incorporation of 13Calpha chemical shift, …
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Glucose dynamics in rat skeletal muscle : recovery from osmotic stress
… glucose kinetics in rat skeletal muscle during iso-osmotic recovery from hyper- and hypo-osmotic stress. Rat EDL muscles were incubated for sixty minutes in either HYPO (190 mmol/kg), ISO (290 mmol/kg), or HYPER (400 mmol/kg) media (Sigma medium-199, 8 mM glucose) according to an established in …
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THERMODYNAMIC AND KINETIC PROPERTIES OF CYTOCHROMES C AND C’ IN THE DENATURED STATE: PROPENSITY FOR RESIDUAL STRUCTURE
… 10 to 111 residues and to provide direct comparison to previous studies with pWT Cytc’ . We observe decreased global stability for most variants and an average decrease of the midpoint of gdnHCl unfolding for A83S/A87S variants compared to pWT. Loop stability versus loop size data yields a …
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THERMODYNAMIC AND KINETIC PROPERTIES OF CYTOCHROMES C AND C’ IN THE DENATURED STATE: PROPENSITY FOR RESIDUAL STRUCTURE
… 10 to 111 residues and to provide direct comparison to previous studies with pWT Cytc’ . We observe decreased global stability for most variants and an average decrease of the midpoint of gdnHCl unfolding for A83S/A87S variants compared to pWT. Loop stability versus loop size data yields a …
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Structural constraints for the folding, stability and function of cytochrome C
… the investigation of mutant proteins. Two isozymes of cyto-chrome c can be isolated from yeast, iso-1 and iso-2-cytochrome c. The structure of iso-l-cyto-chrome c had been previously determined in this laboratory using X-ray diffraction analyses. In the first part of this study, the …
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Role for Macrophage Migration Inhibitory Factor in Multiple Sclerosis
… ruolo importante nella patogenesi di numerosi disordini infiammatori e autoimmuni. Come tale, è un obiettivo terapeutico interessante per il trattamento di questi disturbi. Diversi dati suggeriscono un ruolo chiave di MIF nella patogenesi della sclerosi multipla (SM). Nei topi con EAE, un …
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Investigation of the Structure and Kinetics of Heme-Containing Proteins Using Magnetic Circular Dichroism and Rapid-Scan, Stopped-Flow Spectroscopy
… on <italic>Saccharomyces cervisiae</italic> iso-1-cytochrome <italic>c</italic> and its F82H, M80C, F82C, and T78C/K79G mutants. UV-vis absorption and MCD spectroscopy were utilized to compare these mutants to the wild-type yeast cytochrome c and its His/Met active site ligand set, as well as …