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Showing 1 to 3 of 3 for “"IS900"”.
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Molecular Typing Of Mycobacterial Isolates Cultured From The Tissue Of Inflammatory Bowel Disease (Crohn's Disease) Patients
… of sequences from the16S rRNA, IS1245, and IS900 genes, belonging to members of the MAC, have been developed and optimized into a common protocol to be used as a rapid and accurate diagnostic tool regarding M. avium complex (MAC) infection. The PCR protocol time was reduced by half, and the …
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Isolamento e detecção molecular de Mycobacterium avium subespécie paratuberculosis (MAP) em rebanhos bovinos leiteiros na região de Viçosa - MG
… à PCR, com dois pares de oligonucleotídeos: IS900BN1/IS900BN2 e ISMav2F/ISMav2B2. Utilizando-se o primeiro par, 8 amostras (3,6%), das quais duas eram de animais com sinais clínicos semelhantes aos da doença, amplificaram fragmentos de tamanho esperado, de 626pb, e utilizando-se o segundo …
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Real Time RT-PCR for Direct Detection of Viable Mycobacterium Avium Subspecies paratuberculosis in Chron's Disease Patients and Association of Map Infection with Downregulation in Interferon-Gamma Receptor (INFG1) Gene in Crohn's Disease Patients
… (RT-PCR) assay based on targeting mRNA of the IS900 gene unique to MAP has been developed. All variables included in RNA isolation, cDNA synthesis and real time PCR amplification have been optimized. Oligonucleotide primers were designed to amplify 165 bp specific to MAP and the assay …