Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"IRP2"”.
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Iron Dysregulation and Inflammation in Alzheimer’s Disease
… transgenic iron regulatory protein 2 knockout (IRP2-/-) mice in the early stages of neurodegeneration and in affected brain regions from AD patients at different stages of the disease. IRP2-/- mice demonstrated a region specific deficiency in the metabolically active iron pool, loosely bound …
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Elucidating the role of Mitoferrin (Mfrn), Iron Regulatory Proteins (IRP1 and IRP2) and Hephaestin (Heph) in Iron Metabolism by tagSNP and Protein-Protein Interaction (PPI) analysis
Precisely how Hephaestin (Heph) facilitate iron release from cells is poorly understood. The work in this thesis tried to establish the role of different iron metabolic proteins, Mitoferrin (Mfrn), IRPs and Heph in iron homeostasis. Analysis of 18 tagSNPs in the Mfrn gene was carried out in an …
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The Regulation of Cellular Iron Metabolism
… endogenously tagging Iron Regulatory Protein 2 (IRP2) to the fluorescent protein Clover. I then undertook parallel genome-wide CRISPR/Cas9 mutagenesis screens in HeLa and A549 IRP2-Clover cells to identify genes that when mutagenised disrupt normal iron homeostasis. These screens have allowed me …
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Crosstalk Signaling Between Circadian Clock Components and Iron Metabolism
… a master regulator of iron concentration in IRP2. IRP2 showed rhythms in phase with circadian component PER2, and IRP2's rhythmicity was lost under iron overload conditions. We observed that the ability of these three critical iron metabolic components to respond to sudden increases in …
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Misregulation of Iron Handling Proteins in Excitotoxic Brain Injury
… hippocampus.A sustained, upregulation of IRP1, IRP2, DMT1 and -IRE DMT1 protein was detected in astrocytes in the kainate lesioned hippocampus. An increase in ferritin expression, and increased level of ferric iron and ferrous iron were also observed in microglia and oligodendrocytes in the …
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Comparison of prevalence of avian pathogenic Escherichia coli in South African and US poultry
… PCR for five APEC VAGs (cvaC, iss, iucC, tsh and irp2). The pathogenicity of each isolate was determined by comparing the number of genes detected in each isolate to a positive control. Isolates with two or more virulence genes were considered APEC positive. This research provides supporting …
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MicroRNA Function in Cellular Stress Response
… post-transcriptional regulation of the IRP2 regulon in the cellular response to iron deficiency is well characterized. Here we examine the potential role for microRNA-mediated regulation in the coordinated response to cellular iron deficiency.</p>
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Molecular and cellular insights into iron regulation
… unknown CHO-gene sequences of Hfe, Slc40-a1 and Irp2, was achieved. To determine the effect of intracellular iron overload on hepcidin expression, recombinant transferrin receptor 1 (rec-TfR1) HepG2 cells were created which express modified TfR1 to maximise iron uptake. Upon holotransferrin (5 …
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From yeast to animals: A small molecule restores iron mobilization, absorption and hemoglobinization
… regulatory networks, with levels of ferritin, IRP2, TfR2, and FPN1 responding to changes in the dynamic iron status upon hinokitiol treatment. Administration of hinokitiol via oral gavage promotes gut iron absorption in DMT1-deficient Belgrade (b/b) rats and FPN1- deficient flatiron (ffe/+) …
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V-ATPase regulation of Hypoxia Inducible transcription Factors
Metazoans have evolved conserved mechanisms to promote cell survival under low oxygen tensions by initiating a transcriptional cascade centered on the action of Hypoxia Inducible transcription Factors (HIFs). In aerobic conditions, HIFs are inactivated by ubiquitin-proteasome-mediated degradation …