Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"IPTG-inducible"”.
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Characterisation of an expression system for commercial production of proteins / by Lene Jorgensen.
… expression under control of stationary-phase inducible promoters is characterised, and the expression levels are quantitatively compared with those under control of IPTG-inducible promoters. A number of bacterial expression systems are constructed using promoter::cat transcriptional fusions.
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Construction and analysis of novel controllable expression vectors for Bacillus subtilis
… are still missing. To fill this gap, a glycine-inducible expression system and a lysine-autoinducible one were explored and IPTG-inducible expression plasmids that allow overexpression and purification of proteins were constructed and analyzed. Furthermore, a technique with a useful …
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The development of transgenic plants resistant to cucumber mosaic virus and tobacco necrosis virus
… the maltose binding protein (MBP), in the IPTG-inducible pMALTM vector system, and expressed in E. coli. Through immunological detection the authenticity of both CPs was confirmed. The CMV CP translation product expressed in E.coli was used as an antigen to raise antiserum free from …
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Construction of an efficient secretion system for recombinant proteins in Bacillus subtilis
… the Sec pathway. Its gene amyQ was fused to an IPTG-inducible promoter. It turned out that increasing amounts of IPTG did not result in a concomitant increase of secreted a-amylase. Overproduction either formed aggregates within the cytoplasm or preproteins targeted to the translocon jammed the …
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Function of the ATP-dependent Metalloprotease FtsH during sporulation in Bacillus subtilis
… it describes the construction of a cold-inducible expression system. The major finding of this thesis is that the FtsH protease interferes with the synthesis and/or phosphorylation of Spo0A, the master regulator during initiation of sporulation called phase 0. In the ftsH knockout, the …
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Development of Bacillus subtilis spores and cells for surface display of proteins
… substitution of native promoter by two different IPTG-inducible promoters to the increase the production of fusion protein is presented here. CotB was used and the expression of the cotB gene was regulated by either its own promoter, the Pgrac and the PSgrac promoter in a series of plasmids which …
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Engineering Alkane-Inducible Fimbriation in Escherichia Coli
… fimbriae in response to the presence of IPTG and DCPK. The over-expression of fimbriae in E. coli resulted in a pronounced response to the agglutination of mannose-containing yeast cells, causing them to sediment from solution at a much faster rate than wild-type or fimbrial deletion …