Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"INS-1 Cell"”.
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The effect of iron metabolism on palmitate-induced INS-1 cell death
… A. MATERIALS 24 1. Reagents 24 B. METHODS 25 1. Cell Culture 25 2. Preparation of palmitate 25 3. Isolation of islets 25 4. MTT-bases viability assay 26 5. Measurement of cell death 26 6. Western blot analysis 27 7. RNA extraction and microarray analysis 27 8. Reverse transcriptase-polymerase …
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The Role of [beta]2-Syntrophin Phosphorylation in Secretory Granule Exocytosis
The trafficking of insulin secretory granules(SGs) of pancreatic b-cells is a tightly controlled complex network. Increasing evidence indicates that the cortical actin cytoskeleton modulates the mobility and exocytosis of SGs,yet the mechanisms anchoring SGs to the cytoskeleton is not completely …
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Studies of TNF- α-induced -GSIS Inhibition in INS-1 β-cell
BACKGRAUND AND AIMS: Pancreatic β-cell which produce and secrete insulin, is responsible for regulation of plasma glucose concentration. GSIS depends on coordinated glucose uptakes, oxidative metabolism nd Ca(2+)+-triggered insulin exocytosis. Imparied GSIS is a hallmark of type Ⅱ Diabetes. It has …
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Identification of Substrates and Pathways Regulated by WNK1
… Syt2 within its calcium binding C2 domains. Calcium strongly enhanced their binding in vitro. Essential Ca2+-binding residues in the Syt2 C2 domains were critical for formation of a WNK1-Syt2 complex and for Syt2 phosphorylation. WNK1 displayed specificity among Syt isoforms and …
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Novel Roles of Gastrin and Cholecystokinin in Islet Beta Cell Proliferation
… of Type 2 diabetes share a common theme: an insufficient beta cell mass to maintain glucose homeostasis. In Type 1 diabetes this deficiency arises from autoimmune destruction of the beta cells. In the latter stages of Type 2 diabetes, there is a precipitous drop in beta cell mass, resulting …
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Protective effect of Nicotinamide on High glucose (HG)/Palmitate (PA)-induced Glucolipotoxicity to INS-1 beta cells
Prolonged exposure of pancreatic beta cells to excessive levels of glucose and fatty acids is postulated to contribute to beta cell dysfunction and beta cell death in type 2 diabetes. Nicotinamide, via its major metabolite NAD+, is involved in a wide range of biological processes, including the …
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Cellular Mechanism of High Glucose/Palmitate-induced INS-1 Beta Cell Death
… free fatty acids (FFAs) induces pancreatic beta cell dysfunction and death, which may contribute to the development of type 2 diabetes. A deficiency of insulin through loss of beta cell mass, and subsequent impaired compensation against insulin resistance is believed to be a pathogenic cause of …