Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"INS-1"”.
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The effect of iron metabolism on palmitate-induced INS-1 cell death
… (OCR) 30 13. PA uptake 31 14. Quantitation of insulin 31 15. Immunocytochemistry 32 16. Statistics 32 Ⅲ. RESULTS [Part I] 36 A. Palmitate treatment reduces intracellular iron level in INS-1 beta cell. 36 B. Iron depletion and supplementation in palmitate-induced INS-1 cell death. 41 C. Toxic …
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Cellular Mechanism of High Glucose/Palmitate-induced INS-1 Beta Cell Death
… development of type 2 diabetes. A deficiency of insulin through loss of beta cell mass, and subsequent impaired compensation against insulin resistance is believed to be a pathogenic cause of type 2 diabetes. Pancreatic β-cell mass is regulated by at least four independent mechanisms(i) β-cell …
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Studies of TNF- α-induced -GSIS Inhibition in INS-1 β-cell
… Pancreatic β-cell which produce and secrete insulin, is responsible for regulation of plasma glucose concentration. GSIS depends on coordinated glucose uptakes, oxidative metabolism nd Ca(2+)+-triggered insulin exocytosis. Imparied GSIS is a hallmark of type Ⅱ Diabetes. It has been reported …
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Dysfunction of Pancreatic INS-1 Beta-Cells Induced by Chronic Exposure to High Glucose
… 베타세포에 악영향을 끼치는 것으로 알려져 있다. 본 연구에서는 백서의 베타세포주인 INS-1 세포에서 고농도 포도당에 지속적으로 노출되었을 때 인슐린 유전자 발현과 그의 전사에 중요한 전사인자들 중의 하나인 pdx-1 유전자 발현이 억제되는 데, 이와 관련된 기작을 연구하고자 하였다. 대상 및 방법: 고농도 포도당의 지속적인 노출에 의하여 베타세포의 중요한 기능 중 하나인 인슐린 유전자 발현이 감소하는 지를 확인하기 위해, 백서의 소도세포와 세포주, 마우스 세포주를 대상으로 RT-PCR 방법을 이용하여 인슐린 및 주요 …
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Genistein-Induced Potentiation Effect on Glucose-Stimulated Insulin Secretion (GSIS) in INS-1 β-cells
Impaired secretion of insulin as well as insulin resistance is a critical characteristic of type 2 diabetes. Thus, correction of the deficient secretion of insulin as well as improvement of insulin action is an important target for prevention of overt type 2 diabetes. Sulfonylureas have been used …
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Protective effect of Nicotinamide on High glucose (HG)/Palmitate (PA)-induced Glucolipotoxicity to INS-1 beta cells
… (NAM) significantly protected the cells against the HG/PA-induced cell death. The aim of this study is to determine which mechanism of protective effect of nicotinamide on high glucose/palmitate (HG/PA)―induced INS-1 beta cell death. Protective effect of NAM was due to its role as an …
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Studies on the mechanism of insulin signaling reduction in high glucose/palmitate-induced INS-1 beta cell death
… 독성 (Glucolipotoxicity) 2 B. 고농도 포도당과 지방산이 INS-1 베타세포에 미치는 영향 2 1. 대사관점 2 2. 유전자 발현 2 3. 신호 전달 3 C. 고농도 포도당과 지방산에 의한 IRS-1 tyrosine 기 감소의 원인 4 1. IR (insulin receptor) 에 의한 IRS-1 tyrosine기 인산화 감소 4 2. IRS-1 Serine 인산화가 증가시킴으로 인해 IRS-1 tyrosine 인산화 를 감소 5 3. PTPs에 의해서 IRS-1 tyrosine 인산화가 감소 7 …
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IL-1β Amplification of Nitric Oxide Production and Its Inhibitory Effects on Glucose Induced Early Growth Response-1 Expression in INS-1 Cells
… oxide was measured with a Griess Reagent kit and insulin levels via ELISA. Glucose induced both insulin and Egr-1 production in INS-1 cells. IL-1▀ dose dependently increased nitric oxide production over time and significantly attenuated glucose induced Egr-1 expression. Sodium nitroprusside dose …
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Identifikation und Charakterisierung neuer Leptin regulierter Gene in der Beta-Zelle des endokrinen Pankreas
… des Hypothalamus. Zusätzlich hemmt es die Insulinsekretion der Beta-Zelle des endokrinen Pankreas. Umgekehrt fördert Insulin die Leptinsekretion des Fettgewebes. Dieser Regelkreis wird als adipoinsuläre Achse beschrieben. Fehlregulationen dieses Regelkreises werden bei übergewichtigen …
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The Role of [beta]2-Syntrophin Phosphorylation in Secretory Granule Exocytosis
The trafficking of insulin secretory granules(SGs) of pancreatic b-cells is a tightly controlled complex network. Increasing evidence indicates that the cortical actin cytoskeleton modulates the mobility and exocytosis of SGs,yet the mechanisms anchoring SGs to the cytoskeleton is not completely …
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The impact of glucotoxicity on pancreatic β-cell function
… β-cells and cause impaired glucose-stimulated insulin secretion (GSIS). The aim of present thesis was to investigate the impact of long-term hyperglycemia on the pancreatic islet β-cell function. The data showed abnormally increased expression of inducible nitric oxide synthase (iNOS) protein …
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Effects of Ca2+, microRNAs, and rosuvastatin on insulin-secreting beta cell function
… a condition of high blood glucose levels due to insulin resistance and defective insulin secretion. Impaired insulin secretion plays a major role in the pathophysiology of T2D, it is mainly attributed to beta cell function i.e. failure to secrete insulin or reduced beta cell mass. The exocytotic …
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Kontrollierte Expansion von Insulin produzierenden Beta-Zellen des endokrinen Pankreas durch das Protein p8
… Durch ein Deletionskonstrukt des Fusionsproteins wurde ein nukleäres Lokalisationssignal (NLS) am C-terminalen Ende des Proteins identifiziert. Um weitere Informationen über Funktion und Wirkungsweise von p8 zu erhalten, wurden induzierbar stabile INS-1-Zellen (beta-Zelllinie) etabliert. …
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Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP.
… in the western world. It is characterized by insulin resistance and defects in insulin secretion. Insulin resistance is tightly linked to obesity, and usually precedes the onset of type 2 diabetes. When insulin resistance develops, the pancreatic beta-cells compensate by in¬creasing their …
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Role of PARK2Gene in Maintenance of Pancreatic Beta Cell Functions
… 2 diabetes (T2D) is characterized by decreased insulin secretion and action. Although many studies have reported the genetic loci/genes for susceptibility to T2D, the vast majority of genetic factors in T2D are still unknown. Mitophagy, a selective elimination system for impaired mitochondria, …
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iPLA2β, ALTERNATIVE SPLICING AND APOPTOSIS OF PANCREATIC ISLETS
… (NSMase), an enzyme that hydrolyzes sphingomyelins to generate ceramide, is regulated by Group VIA phospholipase A2 (iPLA2β)-dependent mechanism during β-cell apoptosis. This prompted us to hypothesize that iPLA2 is upstream of ceramide generation in the process regulating splicing of apoptotic …
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Molecular Characterization of Inositol Monophosphatase Like Enzymes in Arabidopsis thaliana
… and breakdown of inositol (1,4,5)-trisphosphate (InsP3), a potent second messenger involved in many biological activities. Arabidopsis contains a single canonical IMP gene, which was previously shown in our lab to encode a bifuntional enzyme with both IMP and L-galactose 1-phosphatase activity. …
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Novel Roles of Gastrin and Cholecystokinin in Islet Beta Cell Proliferation
… of Type 2 diabetes share a common theme: an insufficient beta cell mass to maintain glucose homeostasis. In Type 1 diabetes this deficiency arises from autoimmune destruction of the beta cells. In the latter stages of Type 2 diabetes, there is a precipitous drop in beta cell mass, resulting …
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Islet Dysfunction In Diabetes
… beta cells to synthesize and secrete sufficient insulin to meet the metabolic demands and the inability of tissues [muscle, adipose and liver] to efficiently utilize the secreted insulin leading to an overall increase in blood glucose levels [hyperglycemia]. As indicated by recent estimates from …
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Transient oxygen consumption rate measurements with the BDT̳M̳ oxygen biosensor system
… thickness. Transient OBS measurements with both INS-1 cells and islets were correlated with stirred chamber OCR measurements conducted in parallel. The two measurements were linearly related and a calibration curve was developed so that OBS transient measurements could be converted to OCR. The …
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