Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 33 for “"IMAC"”.
-
Isolation of immunoglobulins from cheese whey and milk using ultrafiltration and immobilized metal affinity chromatography
… to an immobilized metal affinity chromatography (IMAC) column to isolate IgG. With a 50 ml IMAC column loaded with 17.3 ml of 50 mM CuCl₂ to get 2/3 Cu saturation, 1240 mg of IgG could be isolated with 56% purity. About 152 mg of lactoferrin and 90 mg of lactoperoxidase could be isolated along …
-
Endotoxin removal from protein solutions by immobilized metal ion affinity chromatography
… immobilized metal (ion) affinity chromatography (IMAC) has been developed in this study. Fe^{3+} was charged to a chelating gel that contains iminodiacetic acid (IDA). The IDA- Fe^{3+} IMAC column was exploited to remove endotoxin from protein solutions. This method involves the adsorption of the …
-
Characterization of protein microstructure by various chromatographic techniques
… 2) immobilized metal affinity chromatography (IMAC), were used to characterize the above-mentioned protein structure parameters. The model protein chosen for our work is T4 lysozyme. The protein consists of 164 amino acids with molecular weight ~ 18 kD. SYBYL 7.1 software was used to generate …
-
The isolation and characterisation of MHC-presented peptides from CML-derived cell-lines, with a focus on post-translational modification
… poor downstream compatibility, especially with IMAC, a popular method for phosphopeptide enrichment. As an alternative to the citrate-phosphate elution buffer, a TMA-formate elution buffer is proposed. This was developed for IMAC compatibility, and osmotically balanced and supplemented to …
-
Regulation of tumor necrosis factor-alpha induced apoptosis via posttranslational modifications in a human colon adenocarcinoma cell line
(cont.) phosphoproteomics technology, IMAC/LC/MS/MS, [approximately] 200 phosphosites were identified from HT-29 cells, some of which were detected only from insulin-treated cells. Our phosphoproteomics approach also enabled us to detect alteration of both known and unknown phosphorylation states …
-
Separation and Purification of a Monoclonal Antibody for use in Patients Suffering from Wet Macular Degeneration
… immobilized metal affinity chromatography (IMAC) and various multimodal chromatography mediums. Experiments were conducted to determine the most effective mediums that could be used in the process design. To better analyze the new alternate plans, an economic analysis was conducted which …
-
Structural studies of putative general stress and related proteins from Deinococcus radiodurans
… by immobilised metal affinity chromatography (IMAC) was facilitated by the incorporation of a 6xHis tag and supplemented by a final gel filtration step. Although high purity levels were achieved, imaging by SDS-PAGE analysis identified that DR_1146 was susceptible to stringent proteolysis. It …
-
Establishment of enhanced protein purification strategies for recombinant immunotherapeutics
… MB&I research unit successfully employs a single IMAC step to purify mammalian and bacterially expressed recombinant proteins to successfully supporting a decent number of student projects per year. Such enriched protein preparation does allow preliminary qualitative analysis of such highly active …
-
The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
… Immobilized metal affinity chromatography (IMAC) was employed to purify the protein for structural analysis by mass spectrometry and to develop a functional transporting system, VGLUT1 proteoliposomes. Transmembrane topology and homology models of VGLUT1 were generated by web-based and …
-
The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
… Immobilized metal affinity chromatography (IMAC) was employed to purify the protein for structural analysis by mass spectrometry and to develop a functional transporting system, VGLUT1 proteoliposomes. Transmembrane topology and homology models of VGLUT1 were generated by web-based and …
-
Development of recombinant immunotoxins targeting overexpressed cell surface biomarkers trophoblast cell-surface antigen 2 and mesothelin for cervical cancer treatment
… immobilized metal affinity chromatography (IMAC) and quantified. Purified proteins were characterized by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analysis. The rITs were subsequently tested for their ability to bind to antigen-positive cervical …
-
Biosynthesis of the Nitrogenase FeMo-cofactor from Azotobacter vinelandii: Involvement of the NifEN complex, NifX and the Fe protein
… of immobilized metal-affinity chromatography (IMAC). NifEN obtained in this way was characterized using a variety of biophysical techniques and found to contain two [4Fe-4S] clusters in each NifEN tetramer. These clusters were also shown to be completely ligated by cysteine residues. With the …
-
Production and Characterization of an Human Monoclonal Antibody IgG to TRAIL Receptor 2
… DR5-18 IgG를 배양액으로부터 AKTA prime plus 와 HitrapTM IMAC column을 이용하여 정제하였으며 western blotting을 통해 확인하였다. MTT assay, Annexin V-FITC/PI staining, FACS analysis, sub-G1 분석을 통해 DR5-18 IgG가 림프 암세포 주 Jurkat 에서 apoptotic cell death를 유도하는 것을 확인 하였다. DR5-18 IgG는 Fc 분절을 가지고 있으므로 in vitro 상에서 IgG 단독 처리에 비해 보체와 …
-
Characterizing the Phosphorylation State of Tie2 using SH2 Domain Fusion Proteins
… were used with cobalt activated IMAC beads, we were able to precipitate Tie2 in overexpressed systems using the SH2 domains of Shp2 N-C and Grb2. As expected, phosphorylation of Tie2 in the presence of its orphan receptor Tie1 was attenuated compared to wild-type levels. Based …
-
Generation of recombinant SNAP-tag based antibody fusion proteins for immunotherapy of acute myeloid leukemia (AML)
… using immobilized metal affinity chromatography (IMAC). SDS PAGE and Western blot analysis were used to characterise the fusion proteins, facilitated by an N-terminal 10x Histidine (His) tag. The αCD33(scFv)-SNAP-Alexa 488 and αCD45(scFv)- SNAP-Alexa 488 fusion proteins were assessed for cell …
-
Demonstration and implementation of thermally passive low-income housing : a case study in Bhuj
… 60% of operating hours and remained within the IMAC 80% acceptability range 88% of operating hours in the Bhuj climate. In the context of India, the discoveries from this case study in Bhuj can be used to write building guidelines to holistically improve the thermal comfort in Indian homes as a …
-
Development of SNAP-tag-based fusion proteins targeting HIV-1 viral reservoirs
… of compatible solutes. J3-SNAP was purified by IMAC and assessed by SDS-PAGE and Western blot analysis. To ascertain the binding of J3- SNAP to cells expressing HIV-1 Env in vitro, recombinant Env protein was transiently transfected into HEK293T-cells to generate an Env expressing cell line. …
-
Rekombinantinio TDP-43 baltymo sintezės ir gryninimo optimizavimas /
… imidazole concentrations gradient during IMAC and the appropriate pH level. Control of these factors is essential for efficient extraction and purification of target proteins in recombinant protein assays. The data and conclusions of this work may help to develop a stable protocol for …
-
Application of Different Bioanalytical Workflows for Proteomics of Prostate Cancer
… immobilized metal ion affinity chromatography (IMAC); analysis of the enriched digests by LC-MS/MS; and identification of the phosphorylated peptides/proteins through searches of the Swiss-Prot protein sequence database. With in-gel IEF based analytical platform, we have characterized over 600 …
-
Toward a Quantitative Analysis of PARP-1 and Poly(ADP-ribosyl)ation in Cellular Senescence
… and immobilized metal affinity chromatography (IMAC) for the preparation of poly(ADP-ribosyl)ated protein samples prior to MS analysis using both MALDI-TOF and Q-TRAP LC-MS. Based on this work, we developed a three-tiered scheme that may provide the first ever identification of poly(ADP- …
Page 1 of 2