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Showing 1 to 20 of 24 for “"IHF"”.
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Deconstructing Ihf-Mediated Inhibition of the Complex acs Promoter
… acsP2 promoter and that the nucleoid protein IHF binds three specific sites located just upstream. The most proximal site (IHF III) exhibits reduced transcription compared to the full-length promoter or to a construct lacking all three IHF sites. The goal of my research was to understand how …
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An Analysis of Bacteriophage Lambda Site-Specific Recombination Using IHF Binding Site Mutants
Integration Host Factor (IHF) is an Escherichia coli protein required for integration and excision of bacteriophage lambda. Mutations of the three sites to which this protein binds within the lambda attachment site were constructed using oligonucleotide-directed site-specific mutagenesis and were …
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Cooperative Assembly of Terminase and Integration Host Factor at the Packaging Initiation Site of Bacteriophage Lambda
… the role of E. coli integration host factor (IHF) in mediating the site-specific assembly of bacteriophage lambda terminase at its cognate DNA site, cos, which serves as the packaging initiation site. IHF binds to an I-element within cos and introduces a strong bend in the duplex. It was …
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Associations between dietary intake, physical activity and intrahepatic fat.
Excess accumulation of intrahepatic fat (IHF) is directly involved in the pathogenesis of insulin resistance and dyslipidemia, which are risk factors for type 2 diabetes and cardiovascular disease. To our knowledge, no prior groups have examined the inter-relationship between diet quantity, …
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Integration Host Factor and Its Interaction With the H-Prime Site of Bacteriophage Lambda
Integration Host Factor (IHF) is a sequence-specific DNA-bending protein of Escherichia coli that recognizes the DNA through contacts made to the backbone and the minor groove. IHF binds specifically to the H$\sp\prime$ site, $5\sp\prime$-TAAAAAAGCATTGCTTATCAATTTGTTGCAACGA-$3\sp\prime$, with a $\rm …
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Biochemical and genetic analysis of nucleic acid-protein interactions
The integration host factor (IHF) of Escherichia coli is a small, sequence-specific DNA-binding protein. The specific and nonspecific DNA binding constants were determined by gel mobility shift assays. The binding constant of IHF for the H$\sp\prime$ site in $\lambda$ attP is 6.8 $\times$ 10$\sp8$ …
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A study of Escherichia coli integration host factor interaction with DNA
The sequence elements required for IHF binding to the H$\sp\prime$ and H1 sites in attP of $\lambda$ were examined. The H$\sp\prime$ site naturally contains a dA+dT element 5$\sp\prime$ to the core consensus element WATCAANNNNTTR, while the H1 site does not. It was found that both elements are …
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Studies On The In Vitro Transposition Of Bacteriophage Mu Dna
… protein was shown to be integration host factor (IHF). Low levels of IHF also reduce the amount of HU and Mu A required for the reaction and at high concentrations of IHF, HU is not required. The SRF activity of IHF is mediated through an IHF binding site in the Mu early promoter region. This site …
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ROLE OF CIRCULAR PVT1 IN ISCHEMIC HEART FAILURE
… and their respective functions in ischemic HF (IHF) remain largely unknown. This study aimed to identify circRNA-based ceRNA networks in non-end-stage IHF. Transcriptome analysis of left ventricles of IHF patients identified significant differentially expressed circRNAs, miRNAs and mRNAs. …
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Bacteroides Conjugative Transposons: Ecology and Mechanisms
… binding of IntDOT is enhanced in the presence of IHF. We propose that IHF bends attDOT DNA during recombination, facilitating the binding of CTnDOT integrase to attDOT. Using a combination of the in vivo integration assay and the gel shift assay, attDOT has been narrowed to 266 bp. We have …
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DNA-Protein Interactions in f1 DNA Replication
… sites for the E. coli integration host factor (IHF). Both activator proteins bend the DNA sequence to which they bind, implying that together they wrap the origin DNA into a higher order structure that is active in initiation. The replication initiator protein of bacteriophage f1 (gene II …
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Osteogenesis During Early Healing Around Titanium and Titanium Zirconium Implants: Immunohistochemistry and mRNA Expression of Bone Markers in Miniature Pigs
… can be analyzed by immunohistoflourescence (IHF). A pilot study was performed to evaluate the expression of the osteogenic genes/biomarkers, and the quantity/quality of bone in contact with the implant during the early healing around titanium SLA, titanium SLActive, and titanium zirconium …
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H-NS Counter-silencing as a Primary Regulatory Mechanism of Curli Gene Expression in Salmonella Typhimurium
… through transcriptional regulators such as CsgD, IHF, and MlrA. I have identified an essential role of CsgD as a direct counter-silencer to H-NS silencing. Using a suite of footprinting techniques in vitro, I was able to determine that CsgD causes a topological distortion to disrupt the H-NS …
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The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT
… Purified E. coli integration host factor (IHF) can substitute for the Bacteroides host factor(s) and significantly stimulates in vitro integration.
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A genetic analysis of bacteriophage lambda integrase-core site interactions
… contain binding sites for Int as well as for IHF, Xis, and FIS. This array of protein-DNA interactions forms a higher-order complex, the intasome, which is the active substrate for recombination.
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Oxygen Regulated Genes in Salmonella Typhimurium
… was affected by mutations in fnr, hip (IHF), crp, lrp, ackA or pta. NMR analysis of the cultures of a wildtype strain, grown anaerobically in a medium containing threonine, aspartate, leucine and casamino acids, showed that S. typhimurium produced propionate from threonine.
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Transposable prophage Mu exists as an independent chromosomal domain in E. coli
… and the E. coli nucleoid-associated proteins IHF, Fis and HU. The Mu domain was observed at two structurally different chromosomal locations, and was specific to the Mu prophage, i.e. was not observed for the [mathematical symbol] prophage. A model is proposed that by employing its …
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Characterization of the Gifsy-1 Phage Excisionase
… and accessory protein, Integration Host Factor (IHF). This forward recombination reaction between phage encoded attP and host encoded attB is referred to as phage integration. Integration results in the creation of two attachment sites that border the integrated phage chromosome, attL and attR. …
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Regulación simbiótica de la expresión del sistema hidrogenasa por NifA en R. leguminosarum bv. viciae
… cuya expresión es dependiente de RpoN, de IHF y del activador transcripcional NifA. La activación dependiente de NifA de P1, fue corroborada en el fondo genético de K.pneumoniae mediante experimentos de inhibición de la nitrogenasa por múltiples copias de P1, y en el fondo de R.etli …
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