Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 125 for “"IGF-I"”.
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Cytokine-Induced Igf-I Resistance in Progenitor Muscle Cells
… between the endocrine and immune systems. IGF-I is a critical anabolic factor that promotes growth and regeneration of skeletal muscle. Conversely, elevated levels of proinflammatory cytokines are associated with reduced anabolic activity of IGF-I and increased loss of muscle mass that …
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Mechanisms of Cytokine-Induced IGF -I Insensitivity in Cancer Cells
… (IL-1beta) and interleukin-6 (IL-6), inhibit IGF-I-promoted DNA synthesis in human MCF-7 breast adenocarcinoma cells, T-47D breast ductal carcinoma cells, murine FDCP progenitor myeloid cells and human promyeloid HL-60 cells. Flow cytometry confirmed that all three cytokines suppress …
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The effect of insulin-like growth factor-I (IGF-I), and an IGF-I-like factor secreted by human lung fibroblasts, on the growth of human lung carcinoma cells in vitro
… concentration of insulin-like growth factor I (IGF-I) in tissue taken from human non-small cell lung carcinoma (NSCLC) is 1.4- to 7-fold higher than the concentration of IGF-I in the surrounding normal lung tissue and therefore IGF-I may be involved in the growth of NSCLC. In this study it was …
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Regulation of IGF-i Signaling by SOCS3 in Human Coronary Artery Smooth Muscle Cells
… in inflammation. Insulin-like growth factor (IGF-i) is one of the most potent inducers of proliferation in hCASMCs, mainly through the activation of phosphatidyl inositol3 (Pl3)-Kinase and mitogen induces protein (MAP) Kinase pathways. Cytokines on the other hand use other pathways such as …
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Chondrocyte Regulation by IL-I and IGF-I: Interconnection Between Anabolic and Catabolic Factors
… (IL-1) and insulin-like growth factor-I (IGF-I). These factors have antagonistic effects on chondrocyte activity, and during the progression of osteoarthritis, IL-1 is thought to promote chondrocyte hyporesponsiveness to IGF-I. To better understand how the anabolic (IGF-I) and catabolic …
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Exploring Chondrocyte Integrin Regulation of Growth Factor IGF-I Expression from a Transient pAAV Vector
Insulin-like Growth Factor I (IGF-I) is a growth factor that stimulates both mitogenic and anabolic responses in articular chondrocytes. While it has been shown that exogenous IGF-I can regulate chondrocyte integrins, little is known regarding regulatory effects of IGF-I produced from a transiently …
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Regulation of Bovine Mammary Epithelial Cell Response by Autocrine IGF-I and by Collagen I
Understanding how insulin-like growth factor-I (IGF-I) signaling in mammary epithelial cells may be modified or interrupted by modifications in the cellular environment may lead to 1) methods to increase the growth and proliferation of normal mammary epithelial cells for an increase in the amount …
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Design, production and characterisation of IGF-I analogues with increased gastric stability / by Katherine J. Bryant.
… cleavage sites of purified pepsin in long-R3-IGF-I and assess whether the resulting cleavages affect biological activity, to design and produce analogues of long-R3-IGF-I which contain amino acid substitutions, to characterise the resulting analogues for pepsin resistance and retention of …
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In Vitro Binding and Transport Regulation by Endothelial Cells: Preliminary Studies looking at FIX and IGF-I
… study investigates insulin-like growth factor-I (IGF-I) transport across both BAEC and MAC-T cells, a mammary epithelial cell line, cultured on tissue culture inserts. IGF-I is a circulatory growth factor implicated in the regulation of cell division and tissue proliferation. Competitive binding …
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Overexpression of ovine insulin-like growth factor-I (IGF-I) in the mammary glands of transgenic mice
… the production of insulin-like growth factor-I (IGF-I). To generate a model for investigation of the effects of enhanced IGF-I synthesis in the mammary gland, six transgenic founder mice were produced by microinjection of a cDNA sequence encoding ovine IGF-I (oIGF-I) under the control of the …
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Characterization of the IGF system in food-restricted pregnant and lactating rats: Role of IGF-I in nutrient partitioning
… this adaptation. To investigate the role of the IGF-I in adaptation to dietary restriction, the experiments reported herein determined the changes in the circulating levels, as well as, in the gene expression of IGF-I and IGFBPs in pregnant and lactating rats which were food restricted during …
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Expression of the repressor element-1 silencing transcription factor (REST) is regulated by IGF-I and PKC in human neuroblastoma cells
… of SH-SY5Y human neuroblastoma cells exposed to IGF-I. and phorbol 12- myristate 13-acetate (PMA) To down-regulate REST expression we developed an antisense (AS) strategy based on the use of phosphorothioate oligonucleotides (ODNs). In order to evaluate REST mRNA levels, we developed a real-time …
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Orally Administered IGF -I Combined With Partial Enteral Nutrition Alters Intestinal Morphology and Disaccharidase Activity During Parenteral Nutrition in Neonatal Piglets
… study investigated the mechanism(s) by which IGF-I up-regulates LPH activity. The three treatment groups were: 100% TPN, 20% PEN with or without 1.0 mg/kg IGF-I. IGF-I increased LPH activity and LPH activity per unit mature LPH ∼2-fold over PEN alone, but did not affect LPH mRNA or the …
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Quantification of the Binding of Insulin-like Growth Factor-I (IGF-I) and IGF Binding Protein-3 (IGFBP-3) Using Surface Plasmon Resonance
… in a variety of mammalian cells through the IGF-I cell surface receptor (IGF-IR). A unique feature of the IGF-I system is the regulation of this binding by soluble IGF binding proteins. Recent studies from our laboratory show that there is a pH dependence in the association of IGF-I with the …
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In vitro comparison of equine tendon- and bone marrow-derived cells expanded with FGF-2 prior to culturing with tendon matrix and IGF-I
… tendon matrix and insulin-like growth factor-I (IGF-I). Progenitor cells were isolated from six young adult horses, expanded in monolayers with FGF-2, and cultured with autogenous acellular pulverized tendon and IGF-I for seven days. Initial cell isolation and subsequent monolayer proliferation …
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Autocrine mechanisms of action of insulin-like growth factor-I (IGF-I) and hormonal regulation of expression of IGF-finding proteins in mammary epithelial cells
… expression of insulin-like growth factor-I (IGF-I) and IGF-binding proteins (IGFBPs) genes in mammary epithelial cells. To test the hypothesis that IGF-I affects growth of bovine mammary epithelial cells through an autocrine and/or paracrine pathway, several cell lines were developed …
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Microphysiometry Studies of Rapid Binding of Insulin-Like Growth Factor I by Parental and Transfected Mammary Epithelial Cell Lines
… of the family of insulin-like growth factors (IGFs) are proposed to play a major role in the development and subsequent uncontrolled proliferation of breast cancer cells. Insulin-like growth factor-I (IGF-I) is known to be a potent mitogen for mammary epithelial cells. IGF-I acts by binding to …
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Development of a technique for measuring insulin-like growth factor-I in swine:application to the study of the IN VIVO and IN VITRO effects of growth hormone in neonatal pigs
… hormone (pGH) and insulin-like growth factor-I (IGF-I) in swine was investigated. IGF-I levels were measured by radioimmunoassay (RIA) with an antisera specific for human IGF-I after ultrafiltration of acidified samples. Ultrafiltration quantitatively separated carrier proteins and IGF-I. Failure …
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The Role of Insulin-Like Growth Factor-I in Myeloid Cell Differentiation
IGF-I transcripts and its protein are specifically induced as myeloid progenitor cells undergo differentiation toward macrophages. However, the potential role of IGF-I in myeloid cell differentiation has not yet been investigated. The data in this thesis demonstrate that IGF-I combined with either …
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