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Showing 1 to 2 of 2 for “"I-TevI"”.
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Mechanism of Permissive Cleavage Activity of TevCas12a
… called TevCas12a. TevCas12a is a fusion of the I-TevI nuclease and linker domains to Cas12a. The goal was to create a dual endonuclease that made precise double-strand breaks leaving non-compatible ends for exogenous DNA insertion and specific deletions. TevCas12a was expected to make two …
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DNA hydrolysis and genome editing applications of GIY-YIG family homing endonucleases
… reagents, I fused the nuclease domain of I-TevI to three different re-targetable DNA-binding platforms utilized in the field. The engineered nucleases developed within the present studies are mechanistically distinct from established technologies, as they function as monomers and cleave DNA …