Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"I-Dna"”.
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Mechanistic Characterization of the Mitochondrial Type I DNA Topoisomerase and a Study of Genes Containing Type I DNA Topoisomerase-Related Domains
<p>The mitochondrial type I DNA topoisomerase (mt-topo I) serves an important function in the mitochondrion by relaxing mtDNA supercoils to allow for replication of the mitochondrial genome as well as for gene expression. The mt-topo I's role in essential processes, such as replication and …
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Solution Conformations and Structural Thermodynamics of Type I DNA Polymerases from Escherichia coli and Thermus aquaticus
The global conformations of DNA polymerase I from Thermus aquaticus (Taq/Klentaq) and E. coli (Pol I/Klenow) both in isolation and in complex with DNA have been examined in solution using hydrodynamic (analytical ultracentrifugation) and small/wide angle X-ray scattering techniques and then …
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DNA structural selectivity of binding by the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus
… the thermodynamics of substrate selection by DNA Polymerase I is important for characterizing the balance between replication and repair for this enzyme <I>in vivo</I>. Due to their sequence and structural similarities, Klenow and Klentaq, the “large fragments” of the Pol I DNA polymerases …
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Part I. DNA Base and Base Analogue Pairing in Organic Solvents. Part II. Ring Closing Metathesis of Poly-Allylated Glycerol Dendrimers
Part II details cross linking of glycerol dendrimers using the ring closing metathesis (RCM) reaction. An iteratively cross-linked dendrimer was prepared by RCM of an allylated G-3.5 cross-linked dendrimer and the methodology developed for perfect dendrimers was subsequently applied to …
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Cloning of "Animal Cryptochrome" cDNA from the Model Organism <i>CHLAMYDOMONAS REINHARDTII</i> for Functional Analysis of Its Protein Product
… even higher sequence similarity with the 6-4 DNA photolyase of <i>Arabidopsis</i>. DNA photolyases are involved in the repair of UV light-induced DNA damage using the energy of blue light. In order to determine, if the “animal cryptochrome” gene of <i>C. reinhardtii</i> actually encodes a 6-4 …
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Diagnostics and epidemiology of <i>Phytophthora infestans</i> : the cause of late blight of potato
… and INF REV, a 613 bp product was generated from DNA of an isolate of <i>P. infestans</i>. No product was amplified when DNA from isolates of various other <i>Phytophthora </i>species and potato blemish pathogens was tested, showing that the primers were species-specific. These primers can detect …
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Studies on Species of the Aphid Genus Cinara on Pinus Edulis and Pinus Monophylla
… etc.). In Chapter 1, using cytochrome oxidase I DNA sequences, I performed molecular phylogenetic analysis of Cinara species feeding on pinyon pines and determined that switching hosts played a key role in the speciation of the genus. This was reflected in the fact that species sharing a common …
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Spectroscopic Analyses Of Factors Affecting Formation And Stability Of I-Motif Dna
The four-stranded i-motif (iM) conformation of DNA has importance to a variety of biochemical systems that include nanomaterials and oncogene regulation. Fundamental studies to understand iM formation and its structure in solution still remain to be done. In this dissertation, we will discuss the …
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Studies of G-Protein Coupled Receptors Incorporated Into a Novel, Nanoscale, Membrane-Mimetic System
… particles. We generated apolipoprotein A-I DNA derived from zebrafish (Danio rerio) using molecular cloning techniques and optimized a heterologous bacterial expression system, and protein purification and labeling scheme for obtaining high-yields of pure zebrafish apo A-I. We demonstrated …
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Molecular Analysis of <i>Trypanosoma cruzi</i> Isolates Obtained from Raccoons (<i>Procyon lotor</i>) in Warren and Barren Counties of Kentucky
… use a molecular genotyping approach, followed by DNA sequencing to determine the genotypes (type I, or types IIa-IIe) of 15 of the Kentucky isolates. DNA samples were prepared from 15 <i>T. cruzi</i>- isolates using a Qiagen mini kit, and PCR amplification was performed using published primers for …
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Studies on Type I and Type II DNA Topoisomerases From Mammalian Mitochondria
<p>Mammalian mitochondria contain a unique DNA topoisomerase I with characteristics of both nuclear and bacterial type I DNA topoisomerases. This enzyme, purified from bovine liver, requires cations as cofactors and possesses optimal activity at pH 7.0, 37°C and either 150 mM K+ or Na+ ions. …
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Demonstration of new subtypes of adenovirus 7 in South Africa, and probing oesophageal carcinoma cell lines for evidence of adenovirus or of other oncogenic viruses
… by restriction endonuclease analysis of their DNA genomes. Virus was propagated in human embryo fibroblast cells; genomic DNA, extracted and purified from cellular DNA extracts, was analyzed using 9 different restriction enzymes. Results of this study have demonstrated 2 new genome types of …
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Role of the C-terminal domain of the <font face = "symbol">a</font> subunit of RNA polymerase in transcriptional activation of the <i>lux</i> operon during quorum sensing
… vivo</i> and were chosen for further analysis by DNA mobility shift assays. Results from these assays indicate that while the wild-type <font face = "symbol">a</font>CTD is capable of interacting with the <i>lux</i> DNA fragment tested, all five of the variant forms of the <font face = …
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Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing
… the ability of LuxR to recognize and bind its DNA target (the <I>lux</I> box) or to establish associations with RNA polymerase (RNAP) at the <I>lux</I> operon promoter necessary for transcriptional initiation. A third <I>in vivo </I>assay was used to test the ability of the altered forms of …
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Mechanism of Action and Gold Nanoparticle Delivery of Pt(IV) Prodrugs of Cisplatin
… Pt(II), with the reduction products binding to DNA and other cellular targets. In this work we used pBR322 DNA to capture the products of reduction of oxoplatin, <em>c,t,c</em>-[PtCl<sub>2</sub>(OH)<sub>2</sub>(NH<sub>3</sub>)<sub>2</sub>], <strong>2</strong>, and a carboxylate-modified analog, …
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DNA-Protein Interactions in f1 DNA Replication
<p>This thesis analyzes the interaction of two DNA-binding proteins with the plus strand replication origin of bacteriophage f1. The origin has a bipartite structure consisting of a required core origin region and an adjacent A+T- rich enhancer sequence that potentiates replication approximately …