Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 13 of 13 for “"Hypoblast"”.
-
The role of yolk syncytial layer and blastoderm movements during gastrulation in zebrafish
… of the overlying mesendodermal progenitor (or “hypoblast)” cells. This revealed that, besides the similarity in the overall direction of movement, YSL nuclei and hypoblast cell movements display differences in speed and directionality. Next, the interaction between YSL and hypoblast was …
-
Defining the minimal and context-dependent signalling cascades regulating the maintenance of human naïve embryonic stem cells and early embryo development
… the naïve pluripotent state and induces hypoblast-like cell differentiation. The inhibitory effects of XAV939 (a potent TNKs inhibitor) act in a WNT-independent manner, determined by the emergence of GATA6 cells in either IWP-L6 or DKK1(two inhibitors of WNT signalling). WNT signalling …
-
Illuminating the Black Box: Defining and Modeling Peri-Implantation Human Embryogenesis
… of the epiblast (precursor of the fetus), hypoblast (precursor of the yolk sac) and trophoblast (precursor of the placenta). The blastocyst then implants into the maternal uterus and specifies body axes in preparation for gastrulation. It is thought that most pregnancies fail during the …
-
Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… ICM cell number by IL6 was largely comprised of hypoblast (GATA6+:NANOG-) cells, and most IL6-responsive cells in day 9 blastocysts were hypoblast cells (as measured by STAT3 activation). However, some epiblast (NANOG+) cells were also IL6-responsive, and IL6 appeared to initially slow epiblast …
-
Bovine Pre-gastrulation Development and the role Of Rauber's Layer
… as the formation of the cattle anterior visceral hypoblast (equivalent to the mouse AVE) and cavitation of the epiblast in preparation for epithelialisation of this tissue. Both of these processes are considered pre-requisites for the induction of gastrulation in mammals. The spatio-temporal gene …
-
An in vitro model of human yolk sac and epiblast crosstalk
… equivalent of the mouse AVE in the anterior hypoblast, the hypoblast being the portion of the human yolk sac that lies adjacent to the epiblast. However, the functional role of such a structure within the context of human development is yet to be understood. In vitro stem cell modelling of …
-
Regulation of Zebrafish Gastrulation Movements by slb/wnt11
… It was shown that in slb/wnt11 mutant embryos, hypoblast cells within the forming germ ring display slower, less directed migratory movements at the onset of gastrulation, which are accompanied by defects in the orientation of cellular processes along the individual movement directions of these …
-
Topographie molekularer Faktoren der Keimzellentwicklung während der frühembryonalen Entwicklung des Kaninchens
… erlaubt. Blimp1 wird bereits im Stadium 0 im Hypoblast exprimiert, erste "geblimpte" Epiblastzellen, die wahrscheinlichen PGC-Vorläufer, treten im Stadium 2 in der PGE auf, ihre Anzahl nimmt bis ins Stadium 4 zu, im Stadium 5 sind nur noch wenige nachweisbar. BMP2 bildet im Stadium 1 eine …
-
Variabilität molekularer axialer Differenzierung am Beginn der Gastrulation beim Kaninchen
… zu Grunde liegt, wurden exemplarisch an im Hypoblast exprimierten Schlüsselgenen der frühen Achsenentwicklung Dkk1, Cer1, Lefty und Hex untersucht. Für die Untersuchungen wurde das Kaninchen als Modellorganismus ausgewählt, weil es mit seiner flachen Keimscheibe für den Säugerembryo in …
-
Investigating the Actions of Interleukin-6 Family Members on Post-Hatching Blastocyst Development in Cattle
… increased epiblast (EPI) cell number, reduced hypoblast (HYPO) presence, and increased the EPI:HYPO ratio in d 12 blastocysts. These IL6 effects could not be replicated when IL6 was provided prior to the blastocyst stage (d 5-7), but they could be replicated when IL6 was supplemented after …
-
The zebrafish maternal factor pollywog is required for yolk syncytial layer morphogenesis
… also converge and extend underneath the axial hypoblast. Our work with pollywog ( pwg ) maternal-effect mutants highlights the delicate control of the YSL during yolk morphogenesis, and provides novel insight into understanding which tissues of the embryo are affected by loss of a cohesive YSL. …
-
The Role of Amino Acids and the Threonine-SAM Pathway in the Development of Bovine Inner Cell Mass and Pluripotency
… while H3K9me3 and H3K27me3 were not affected. Hypoblast (PDGRFα), pluripotency-associated epiblast (NANOG, FGF4, and SOX2) and TE (CDX2) markers were not altered in the Qc1 group compared to DMSO controls. Qc1 also severely compromised mouse embryos cultured from day 1-4. In bovine cells, Qc1 …
-
Optimising light-sheet microscopy for high resolution live imaging of the early development of chick embryos
… the visualisation of the largescale epiblast and hypoblast tissue flows that occur during gastrulation, while providing enough spatial and temporal resolution to be able to detect the detailed behaviours of up to 400 000 cells. Furthermore, the microscope was enhanced to be able to measure at …