Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Hyperpolarised"”.
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Preserving hyperpolarised nuclear spin order to study cancer metabolism
… 13C metabolic imaging. However, detection of hyperpolarised substrates is limited to a few seconds due to the exponential decay of the polarisation with the longitudinal relaxation time constant T1. This work aimed to improve the combination of hyperpolarisation and metabolic NMR/ MRI by …
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Clinical and biological validation of hyperpolarised MRI of prostate cancer
… for improving the diagnostic potential of MRI. Hyperpolarised [1-13C]pyruvate MRI (HP-13C-MRI) is an emerging clinical metabolic imaging technique that can probe the exchange of the hyperpolarised 13C label between pyruvate and lactate, a biochemical reaction traditionally viewed as the …
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Mathematical approaches for the clinical translation of hyperpolarised 13C imaging in oncology
… technique which enables the metabolism of hyperpolarised 13C-labelled molecules to be dynamically and non- invasively imaged in tissue. The first molecule to gain clinical approval is [1-13C]pyruvate, the conversion of which to [1-13C]lactate has been shown to detect early treatment re- …
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Detecting tumour responses to treatment using metabolic imaging with hyperpolarised [1-13C]pyruvate and 2-([18F]fluoro)-2-deoxy-D-glucose
… positron emission tomography ([18F]FDG-PET) and hyperpolarised [1-13C]pyruvate magnetic resonance imaging), for the purpose of detecting early responses to treatment in mouse models of cancer. Two mouse models of lymphoma, subcutaneous EL4 tumours and Eμ-Myc transgenic mice, were treated with …
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Interrogating Cancer Metabolism using Novel Imaging Techniques
… and the identification of imaging biomarkers. Hyperpolarised 13C Magnetic Resonance Spectroscopic Imaging (MRSI) is a metabolic imaging technique that enables dynamic imaging of isotopically labelled metabolites in vivo, with [1-13C]pyruvate being the most widely used substrate in pre-clinical …
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Using SABRE in NMR and MRI
… Chapter 4 shows how the method can be applied to hyperpolarised MRI acquisition using phantoms.Chapter 5 discusses the advances obtained in the development of hyperpolarised signals in a biologically compatible solvent system. There are two strands to this chapter, the first being the synthesis of …
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METAL CATALYSED TRANSFER HYDROGENATION, DEHYDROGENATION AND RACEMISATION OF AMINES
… Finally, we describe a method for producing hyperpolarised carbon dioxide gas by using the Cp*Rh(Cl)(TsNCHPhCHPhNH2) ATH catalyst to catalytically decompose a pre-hyperpolarised solution of natural abundance formic acid, which was observed by 13C NMR spectroscopy.
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Imaging and Measuring Cerebral Metabolism in the Healthy Brain and Changes Following Inflammation
… first in man study of the healthy brain with hyperpolarised [1-13C] pyruvate.
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Adenosinergic modulation of hippocampal gamma oscillations: from single cell to whole animal
… showed that \(A_1\)-receptor activation hyperpolarised resting membrane potential, reduced firing rate and EPSP amplitude and shifted the IPSC reversal potential to more negative potentials. Blocking \(A_1\)-receptors increased pyramidal cell excitability and increased excitatory synaptic …
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A chemical genetic approach to identify new treatments for melanoma
… an increase in the number of viable cells with a hyperpolarised mitochondrial membrane potential was observed. To determine if leflunomide had the potential to be used in combination with other melanoma drugs, it was tested in combination with the MEK inhibitor selumetinib. This combination showed …
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Novel multi-parametric, multi-modality imaging for the assessment of tumour biology in renal cell carcinoma
… in RCC impacts metabolism including glycolysis. Hyperpolarised [1-13C]pyruvate MRI (hpMRI) probed the spatially heterogeneous lactic acid fermentation in nine patients with renal tumours. Paired multi-regional tissue samples and imaging afforded a biological understanding of the hpMRI signal. The …
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Sympathetic control of carotid body excitability in hypertension: A preclinical study
… PG neurones of SH rats, which became hyperpolarised. In conscious SHR, the chemoreflex was attenuated, and systolic blood pressure fell by 16 ± 4.85 mmHg. Immunohistochemistry showed positive expression of α1A- and α1B- adrenoreceptors on both glomus cells and blood vessels within the …
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The effects of anaesthetic agents on synapses of lymnaea stagnalis (L.)
… a lack of anaesthetic response. RPeD1 hyperpolarised and became quiescent in response to the application of high concentrations of GABA (10 3-104M), however at lower doses (1O 8-1O 5M), no effect was observed (p<0.05). Under these conditions simultaneous recordings from VD4 showed …
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Development of Optimised Pulse Sequences for Magnetic Resonance Imaging of Tumour Metabolism
Development of efficient pulse sequences for hyperpolarised 13C imaging: Dynamic nuclear spin polarization of 13C-labeled cell substrates has enabled dynamic measurements of tissue metabolism in vivo using 13C MRSI. The principal limitation of the technique is the short lifetime of the …
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Potential of metabolic MRI to address unmet clinical needs in localised renal cancer.
… Chapter 2: here I investigated the potential of hyperpolarised [1-<sup>13</sup>C]pyruvate MRI (HP <sup>13</sup>C-MRI) to probe metabolic intratumoral heterogeneity in renal cancer. I discovered that within a single tumour, regions of varying degrees of perfusion and Warburg effect are present, …
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Carbon and Deuterium MRI studies of human brain metabolism
Metabolic changes to the brain can be due to brain disease causing impaired functioning. Imaging of the metabolic processes is an emerging area of research that can allow for greater understanding of the brain disease whilst offering clinical potential for improved diagnosis. Hyperpolarized (HP) …
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Development of imaging techniques to assess tumour cell death
Cell death is an important imaging target for assessing early tumour treatment response and the effectiveness of therapy. Traditionally, anatomical imaging modalities measure changes in tumour size (Response Evaluation Criteria in Solid Tumours (RECIST)). More recently these assessments have also …