Global ETD Search
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Showing 1 to 11 of 11 for “"Hylarana guentheri"”.
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QUB-1439: A bioactive peptide from the frog skin secretion of Hylarana guentheri
… was isolated from the skin secretion of Hylarna guentheri. It is the first time to identify the biosynthetic precursor encoding cDNA of QUB-1439 from the skin secretion-derived cDNA library though the mature peptide sequence, FLPLLFGAISHLL-NH2, has been identified before. Subsequently, QUB-1439 …
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The discovery of a novel peptide from the defensive skin secretion of Hylarana guentheri
… QUB-1875 was isolated from Guenther's frog, Hylarana guentheri, and several bioactive and cytotoxic assays were performed to evaluate the biological activities of this peptide. Firstly, mRNA from the lyophilised skin secretion was isolated and the nucleotide sequence encoding the precursor of …
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QUB-1475: A novel bioactive peptide from the skin secretion of frog Hylarana Guentheri
… peptide QUB-1475 derived from the frog species Hylarana guentheri was chosen here to get thoroughly studied. <br/>In this research, the novel temporin-like peptide, with the sequence: FLQHIIGALGFIF-NH2, was discovered in the skin secretion of Hylarana guentheri. This thesis aimed to isolate and …
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The discovery of a novel bioactive peptide identified from the defensive skin secretion of Hylarana guentheri
I was greatly honoured to have had the opportunity to study at Queen’s University Belfast and to complete my academic degree of MPhil. I am extremely grateful to people who supported and helped me during my MPhil period. Without their assistance, I could not have finished my project …
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Identification, isolation and bioactivity assessment of a bioactive peptide QUB-1465 from the skin secretion of Hylarana guentheri
… constructed from the lyophilised secretion of Hylarana guentheri. A replijavascript:void(0);cate of peptide was produced through solid phase peptide synthesis. By use of RP- HPLC and MALDI-TOF MS, the synthesised peptide was purified and its mass charge ratio was further confirmed. QUB-1465 was …
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The discovery and evaluation of a novel bioactive peptide, QUB-3112, from the defensive skin secretion of Hylarana guentheri
… identified in the defensive skin secretion of Hylarana guentheri by using a ‘shotgun’ cloning method. To research the biological functions of QUB-3112, it was synthesised by using solid phase peptide synthesis (SPPS) according to the translated putative mature peptide sequence. The synthetic …
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The Discovery and Functional Evaluation of a Novel Bioactive Peptide, QUB-3411, from the Frog Skin Secretion of Hylarana guentheri
… was obtained from the frog skin secretions of Hylarana guentheri by using ‘shotgun’ cloning. The amino acid sequence was translated and the mature peptide was speculated by comparison with formally reported peptides in NCBI database. It showed strong similarity with antimicrobial Brevinin-2 …
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Identification and functional characterisation of an antimicrobial peptide QUB-1529 from the skin secretion of the Asian frog, Hylarana guentheri
Hylarana guentheri is a species which is widely distributed in the coastal areas of southern China. The skin secretions of frogs contain a large amount of biologically active peptides, which have great research value. The peptide QUB-1529 was isolated from the skin secretions of this frog. This …
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Molecular cloning and bioactivity assessment of a bioactive peptide QUB-1509 from the skin secretion of the Asian frog Hylarana guentheri
… from the skin secretion of the Asian frog, Hylarana guentheri, and belongs to the temporin family, was focused upon. A cDNA library was constructed by using the isolated mRNA from the skin secretion and ‘shotgun’ cloning was employed as an interrogation technique. After the mature sequence …
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The membrane-active antimicrobial peptide from frog skin and scorpion venom: from mechanistic insight to design
… type peptide, brevinin-1GHd, found in frog Hylarana guentheri, skin secretion. Functional screening revealed that brevinin-1GHd exhibited potent antimicrobial, antibiofilm, and anticancer efficacy without inducing obvious haemolysis and cytotoxicity at working concentrations, which made it …
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Identification, molecular cloning and modification of skin-derived bioactive peptides from selected Hylidae and Ranidae
… hypochondrialis, Phyllomedusa baltea and Hylarana guentheri, through ‘shotgun’ cloning. In parallel to this strategy, predicted mature peptides were identified and isolated from skin secretion using HPLC and structures verified by LC-MS. The peptides were then chemically-synthesised to …