Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Hydrogen-Deuterium Exchange Mass Spectrometry"”.
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Application of hydrogen deuterium exchange mass spectrometry in protein-ligand and protein-protein interactions
… paramagnetic resonance (EPR) spectroscopy, and mass spectrometry (MS). Compared with NMR and EPR spectroscopy, MS has less stringent sample requirements, including protein concentration and protein size. Moreover, the mass accuracy, sensitivity, and faster data analysis also have contributed to …
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Characterizing Conformational Dynamics and Catalytic Activity of Enzymes by Hydrogen-Deuterium Exchange Mass Spectrometry
… in gram-negative bacteria, and yeast alcohol dehydrogenase (YADH), responsible for facilitating the hydride transfer to NAD+ for energy production in prokaryotes. In the catalysis of both antibiotics and NAD+ reduction the specific residues involved in each binding mode remain under debate. By …
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Advancing the characterization of glycan dynamics with in-electrospray ionization hydrogen/deuterium exchange-mass spectrometry.
… techniques exist to study carbohydrates, mass spectrometry (MS) remains one of the most widely used methods because it provides structural information. Hydrogen/deuterium exchange (HDX-MS) is a labeling technique that monitors the exchanging labile hydrogen atoms for molecules across …
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Study of the Dynamics of Retaining GT-B Fold Glycosyltransferases Using Hydrogen Deuterium Exchange Mass Spectrometry
… Toelucidate substrate-induced local dynamics, hydrogen-deuterium exchange mass spectrometry(HDX-MS) is used to analyze CgMshA from Corynebacterium glutamicum, a model enzymefrom GT-B fold. In the presence of acceptor substrate inositol-1-phosphate (I1P), decreaseddynamics occur in CgMshA's …
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Biophysical Investigation into the Protein Dynamics Governing the Allosteric Regulation of Plant and Animal 15-Lipoxygenases
… (SLO), has been characterized using hydrogen-deuterium exchange mass spectrometry (HDX-MS), revealing that the addition of the allosteric effector, oleyl sulfate (OS), alters a specific region of the enzyme. Herein, we used a combination of thermodynamic and biophysical techniques …
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Conformational Analysis of Disordered Proteins Using H/D Exchange Mass Spectrometry
… has become apparent. The application of hydrogen/deuterium exchange mass spectrometry (H/D-MS) to study IDPs is presented in this dissertation. Results that demonstrate improvements to the H/D-MS method are also presented. A thermoelectrically cooled refrigeration system was developed in …
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Ligand and Protein Dynamics of CYP3A4
… effects on conformational dynamics of CYP3A4 via hydrogen-deuterium exchange mass spectrometry for the allosteric effector, midazolam (MDZ). The MDZ-induced effects on the structural dynamics of CYP3A4 are not confined to the active site and they include regions remote from the heme active center. …
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Actin-like filament A (AlfA) and AlfA-driven plasmid segregation
… area, which we confirmed by both cryo-EM and hydrogen-deuterium exchange mass spectrometry (HDX-MS). A comparison of AlfA structure with other actins revealed a unique nucleotide binding conformation, which causes AlfA to remain stable after nucleotide hydrolysis. In addition to the filament …
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Computational and Experimental Investigation of Allosteric Communication in the Transcriptional Regulator NikR
… communication between the ACT and RHH domains. Hydrogen/deuterium exchange mass spectrometry shows a Ni<super>2+</super> specific NikR conformational change relative to bound Cu<super>2+</super>, Co<super>2+</super>, and Zn<super>2+</super>. Concurrent coordination geometry and <italic>in …
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The mechanism of Parkin activation by PINK1 phosphorylation
… and crystallographic approaches were employed. Hydrogen–deuterium exchange mass spectrometry reveals that Parkin phosphorylation enables a conformational equilibrium between an autoinhibited and an active Parkin state. In the active state the phospho-Ubl domain binds to the Unique Parkin domain …
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Structural Dynamics and Reaction Reversibility of Specific vs. Promiscuous Detoxification Enzymes
… CYP3A4 and CYP19A1, respectively, via hydrogen-deuterium exchange mass spectrometry and molecular dynamics simulations. Comparison of the structural dynamics of CYP3A4 bound to eight ligands reveals subtle and ligand-dependent effects on dynamics that are primarily distributed in the …
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Characterisation of Prodomains in the GDNF Family
… the cell surface where HS is common. Utilising hydrogen deuterium exchange mass spectrometry (HDX-MS) no interaction between the NRTN prodomain and mature domain could be identified, again unlike other TGF-β proteins. This work highlights the diversity of prodomain structure and function in the …
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FROM ANTIBODY-DRUG CONJUGATES TO MASKED ANTIBODIES: BIOPHYSICAL INSIGHT FOR THE RATIONAL DESIGN OF FUTURE THERAPIES
… manufacture, was characterized. A combination of mass spectrometry and biophysical techniques were used to understand how the additional disulfide bridge forms, interconverts, and changes the stability and structural dynamics of the antibody. Alternative strategies to the use of maleimide linkers …
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Characterization, Stabilization and Formulation Design of IgG and Secretory IgA Monoclonal Antibody Candidates during Storage and Administration
… by both biophysical techniques as well as hydrogen-deuterium exchange mass spectrometry (HX-MS). The aim is to not only characterize mAb molecular properties and solution behavior at relatively high protein concentrations, but also to develop a better mechanistic understanding by …
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Mass Spectrometry-Based Strategies for Biomolecular Structure Analysis: Steroid Metabolites Structures and Protein-Protein Interactions
Mass spectrometry is an important method for studying the structure of both small molecules and large biomolecules (e.g., proteins). The majority of the applications prior to 1970 were focused on small molecules, owing to the limited ionization methods which posed difficulties in producing …