Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Hydrogen Exchange"”.
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Studies of Protein Stability by Hydrogen Exchange-Mass Spectrometry
… of proteins to reduce long development times. Hydrogen Exchange-Mass Spectrometry (HX-MS) is increasingly being used in pharmaceutical industry for studying protein-protein, protein-ligand, and protein-receptor interactions. However, there is much potential for using HX-MS in a variety of …
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PROTEIN HYDRATION DEPENDENCE OF THE AMIDE HYDROGEN EXCHANGE OF LYSOZYME.
The rate of exchange of the labile hydrogens of lysozyme was measured by out-exchange of tritium from solution samples and powder samples at varied hydration levels for pH 2, 3, 5, 7 and 10. The dependence of exchange of powder samples on the degree of hydration was the same at all pH, reaching …
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The kinetics of hydrogen exchange between hydroxyl groups of alcohols
Thesis: B.S., Massachusetts Institute of Technology, Department of Chemistry, 1960
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From Solution Into the Gas Phase: Studying Protein Hydrogen Exchange and Electrospray Ionization Using Molecular Dynamics Simulation
… first examine microscopic phenomena underlying Hydrogen/Deuterium exchange (HDX). HDX interrogates structural dynamics of proteins by measuring the rate of Deuterium uptake into backbone amides. We perform microsecond MD simulations on ubiquitin to investigate this process. We find that HDX …
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High Pressure Effects on the Dynamics and Kinetics of Two Model Biochemical Systems
Pressure effects on rate constants for hydrogen exchange between N-methylacetamide (NMA) and water were measured at pressures up to 5 kbar at three different pH values and temperatures using proton/nitrogen-14 decoupled NMR inversion transfer, and the apparent activation volumes of exchange …
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Experimental characterization of polyalanine helices in short and long contexts
… analytical methods: circular dichroism (CD), hydrogen exchange, and the t/c ratio of Ac-Hel, a helix stabilizing N-cap, are used in combination to quantify fractional helicity in order to determine the intrinsic helical propensity of an alanine residue, free from the hydrophobic packing within …
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NMR Investigations of Structure in Pressure and Cold Denatured Proteins
… used to perform the cold denaturation studies, hydrogen exchange rates in model amides were measured at various temperatures and pressures to determine the effect of the extreme conditions. These reaction rates are compared to those obtained by correcting extant data for temperature and pressure.
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NMR study of the unfolding of ribonuclease A, and dynamical studies of liquids in confined geometries
… time measurements, and the pressure-jump hydrogen-exchange method. It was found that the pressure denatured states of RNase A display some characteristics of a molten globule, and all three $\alpha$-helices and the $\beta$-sheet of the native protein remain partially folded structures in …
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Calmodulin and Calmodulin-Peptide Interactions: Structure, Energetics, and Dynamics
… was investigated further by employing amide hydrogen exchange techniques. Amide hydrogen exchange rates were measured at each site in the CaM-bound peptide. Slowing factors, were determined and indicated the requirement for reorganization of the complex prior to exchange. Ca$\sp{2+}$ binding …
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Stability and Dynamics of Apocytochrome B(562)
… method and those obtained by calorimetry and hydrogen exchange. The dependence of the global unfolding free energy upon pressure is first order and associated with a negative volume change of -105 mL mol-1. The two additional regions of cooperative structure also displayed negative volume …
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Steric and Electronic Effects Induced by Ancillary Ligand Substitutions on Cyclopentadienyl Osmium Complexes
… ligands, effectively raising the barrier to hydrogen exchange in the dihydride and the methyl hydride systems.
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The Role of Dysfunctional Na+/H+ Exchange in the Development of Dysbiosis and Subsequent Colitis
… and the development of inflammation: sodium-hydrogen exchange at the brush border, mediated by sodium hydrogen exchanger 3 (NHE3). NHE3 is inhibited during active IBD, but its loss in knockout animals is also enough to promote spontaneous colitis in a microbiome-dependent fashion. This …
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Multi-mode two-dimensional infrared spectroscopy of peptides and proteins
… residues. Integration of these tools with hydrogen exchange techniques allows for the protein structural kinetics and stability to be observed through protein-solvent interactions with enhanced structural sensitivity relative to amide I spectroscopy alone.
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Pharmacological activation of pro-survival pathways as a strategy for improving donor heart preservation
… oxide) and Cariporide (an inhibitor of sodium hydrogen exchange). Here, cardiac protection was accompanied by activation of the ERK 1/2 pro-survival pathway as well as a decrease in apoptosis. The third study examined the cardioprotective effect of supplementation of Celsior with all three …
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Some physical and chemical aspects of ammoniacal nitrogen in soils
… the curves were related to reactions involving "hydrogen", exchange sites and physical adsorption. The S-shaped curve obtained for high concentrations suggests the formation of polymolecular layers. Ammonia adsorption by neutral and basic soils also gave a typical Freundlich adsorption isotherm …
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Biochemical and Biophysical Characterization of the Allosteric Equilibrium of the Wiskott-Aldrich Syndrome Protein
… that a simple two-state model can predict the hydrogen exchange behavior and binding affinity for Cdc42-GTP of WASP proteins over a range of folding stabilities. The goals of my thesis have been: 1) to further develop the quantitative twostate model of the allosteric regulation of WASP, 2) to …
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Developability assessments and analytical characterization of recombinant protein antigens formulated as low-cost, multi-dose, aluminum-adjuvanted vaccines
… of biophysical characterization assays and hydrogen exchange mass spectrometry (HX-MS) was used to evaluate the effect of thimerosal on the conformational stability, antibody binding, and backbone flexibility of the parent and a cysteine to serine mutant (C173S) of the NRRV P[4] antigen. The …
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B CELL EPITOPE MAPPING OF RIVAX, A CANDIDATE RICIN VACCINE ANTIGEN
… discontinuous/ conformational epitopes. Based on hydrogen exchange mass spectrometry (HX-MS) studies, VHHS recognized overlapping epitopes with four spatially distinct contact regions i.e. they were grouped into four subclusters (namely 3.1 to 3.4) within cluster III region. Subcluster 3.1 …
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STABLE ISOTOPE RATIOS AS A PROXY FOR HUMAN GEOGRAPHIC PROVENANCE: BIOGEOGRAPHICAL EVIDENCE FROM THE 2H, 18O, AND 13C SIGNATURES IN MODERN HUMAN TEETH
… values. While unexpectedly low rates of hydrogen exchange within mineral hydroxyl groups were documented, and it was possible to analyze tooth enamel for its 2H isotopic composition, the seemingly fixed nature of 2H abundance in tooth enamel means that the 2H isotopic signature of tooth …
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Quantum mechanical analysis of donor-acceptor interactions in organometallic complexes and comparative analysis of class size and teacher experience on student satisfaction and learning
… the global minimum is a non-classical dihydrogen structure irrespective of the nature of the phosphine. For bidentate phosphines that are strongly donating, a classical cis-dihydride structure lies 2 kcal mol-1 or higher in energy than the global minimum. For phosphines that are less …