Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Hybridomas"”.
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Part One: Investigation of the Immunogenicity of a Spin Label Probe. Part Two: An Electron Paramagnetic Resonance Study of Dinitrophenyl-Binding Myelomas and Hybridomas Using Spin Labelled Probes
In Part One, the spin label 2,2,5,5-tetramethylpyrrilidin-3-carboxyl-1-oxyl (tmcpo) was investigated for its immunogenicity at attempting covalent attachment of the probe to a carrier molecule. Detectable immune responses to these immunogens in New Zealand adult rabbits could not be observed with …
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Presentation Of Citrullinated Peptides Derived From Unmodified Protein By Antigen Presenting Cells.
… citrullinated epitopes. We generated T cell hybridomas by fusing these T cells. T cell hybridomas that recognize citrullinated peptides from hen egg white lysozyme: HEL) were used to detect presentation of citrullinated peptides. Using this technique, we show that antigen presenting cells: …
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Golgi Specificity and Development of Autoreactive B Cells
… the spleen. Splenic B cells when immortalized as hybridomas show B cell receptor (IgM) accumulation in the Golgi. Receptor accumulates in the Golgi only when the heavy chain is paired with the Vk38c light chain. The location of B cell receptor accumulation in these hybridomas ranges from the …
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Molecular and functional analyses of human synovial B-lymphocytes in rheumatoid arthritis
… VH and VL genes from human synovial B-cell hybridomas and synovial tissue demonstrates somatic mutations due to antigen activation. The FDC formations in the synovial tissue may therefore serve as an environment for B-cell maturation, which is involved in the generation of autoantibodies. An …
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Cell-Surface GRP78 and its Antibodies: Pathologic and Therapeutic Roles in Cancer
… candidate IgGs. We developed three stable hybridomas that produce interesting antibodies. The N88 IgG reacts with the NH2-terminal domain and is an agonist. The C38 IgG reacts with the COOH-terminal domain and is an antagonist of NH2-terminal signaling. The C107 IgG binds the COOH-terminal …
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Processing of Exogenous Hen Egg-White Lysozyme for Presentation on Major Histocompatibility Complex Class I and II Molecules
… and 2) cross-presentation of HEL to CD8+ T cell hybridomas by antigen presenting cells: APCs) from naïve and immunized mice. Non-obese diabetic: NOD) mice immunized with HEL in complete Freund's adjuvant: CFA) primed HEL 11-25-specific CD4+ T cells and HEL 20-35-specific CD8+ T cells; CD8+ T …
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The Role of Interferon Gamma in the Regulation of IL-18 Binding Protein and the Development of Autoimmune Arthritis in a Genetically Non-Susceptible Mouse Strain
… peptides as well as by the generation of T hybridomas generated in I-A<sup>b</sup>-expressing mice that respond to CII. In a second series of experiments, the role of IFN-g in modulating disease progression was examined by the use of microarray analysis to identify genes that are altered …
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Liver Cell Adhesion Molecules in Embryonic Development
… in rabbits and monoclonal antibodies from murine hybridomas. The identity of the L-CAM activity with the isolated molecule was confirmed using these immunological reagents, by demonstrating their capacity for disrupting cell adhesion and by showing that these antibodies were neutralized by the …
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The dependence of CD4 T cell development and activation on the kinetics of the TCR:pMHC interaction
… did not respond strongly to all Hb APLs. N3.L2 hybridomas and double positive thymocytes responded more strongly to two APLs of the P2 TCR contact residue. Therefore the changes between the n3.L2 and M2 TCR structures only allow certain residues to productively bind. By measuring the kinetics of …
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The G protein-coupling specificity of D2-like dopamine receptors
… of G proteins. 4 way primary screening of 1632 hybridomas generated from mice immunized with GTPS-loaded Gi1 and isolated using an automated robotic colony picker, identified 3 antibodies that interacted with the constitutively active Q204L but neither the constitutively inactive G203A nor wild …
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Engineering human single-chain T cell receptors
… were displayed on the surface of T cell hybridomas in the absence of the coreceptor CD8. Selection with fluorescently labeled dimers of WT-1/Db resulted in the isolation of two high affinity 3D TCR variants, one which mediated T cell activity in the absence of CD8. Analysis of the TCR …
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Interaction of the porcine reproductive and respiratory syndrome virus with the macrophage-specific lectin sialoadhesin
… 5) describes the production of PRRSV-specific hybridomas and an extensive characterization of the monoclonal antibodies (mAbs) they produce. A GP4-specific mAb, directed against a well-characterized neutralizing epitope in the GP4 ectodomain, exerted a strong neutralizing effect on PRRSV …
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Immunological and biosynthetic studies of the human pyruvate dehydrogenase complex
… (mAbs) secreted by individual patient-derived hybridomas (IgG/λ) that interact with a common lipoylation-dependent epitope found on both E2 and E3BP lipoyl domains although the precise antigenic determinant has not been fully defined to date. To address this issue, three amino acid residues …
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Contributions of viral and cellular gene products to the pathogenesis and prognosis of aggressive lymphomas
High grade aggressive lymphomas have high mortality. By their nature, more than 40% of patients die from these diseases even with the improved treatment strategies currently available for oncology patients. The characteristic feature is that they are functionally heterogeneous and therefore have …