Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"Hybridoma"”.
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Effects of rapamycin and insulin on the cell cycle and apoptosis of hybridoma cell cultures
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1999.
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Production of Monoclonal Antibodies for Toxoplasma gondii Lactate Dehydrogenase
… of T. gondii, at the first of all, hybridoma cell lines producing monoclonal antibodies with high affinity and specificity against T. gondii LDH (TgLDH) were established. Methods: After 6-8 week old BALB/c mice were immunized with synthetic peptides of TgLDH, splenocytes were …
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Towards an antibody mediated resistance against Geminiviruses
… were generated using phage display and/or hybridoma technologies. Purified AV1 (coat protein) and AC1 (replicase enhancer) viral proteins were used for mice immunization and screening of phage display libraries and hybridoma clones. The binding properties of the isolated scFvs were …
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Comparison of the growth and monoclonal antibody production of suspended mammalian cells in three perfusion systems
… by HPCHO Chinese hamster ovary cells and IB4 hybridoma cells in batch, fed-batch, and three perfusion systems: the stirred ceramic membrane reactor (SCMR), the alternating tangential flow (ATF) hollow fiber membrane system, and the external spin filter (ESF) system. The batch experiments for …
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Generierung, Charakterisierung und funktionaler Assay von Antikörpern und Antikörperfragmenten gegen die HCV Strukturproteine core, E1 und E2
… ordered. In a second step already existing hybridoma clones were screened for their specificity by indirect ELISA. As part of a cooperation project monoclonal antibodies of one of the selected clones were utilised successfully in immunofluorescence analyses. By carrying out a “Two Step …
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Quantitative analysis of the T cell receptor signaling network in response to altered peptide ligands
… to stimulation through the T cell receptor. A hybridoma system was identified that exhibits different levels of both apoptosis and cytokine production in response to three altered peptide ligands. We investigated how the consequent downstream signaling networks integrate to ultimately govern …
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Production of recombinant monoclonal antibodies against HSET
… antibodies (mAbs) against HSET using a hybridoma technique and characterized them. Peptide antigens of 12 amino acid (aa)-length, designed from the regions corresponding to N-terminus (1-134th aa) and C-terminus (626-672th aa) that do not have homology with other kinesin molecules, were …
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Molecular and functional analyses of human synovial B-lymphocytes in rheumatoid arthritis
… VH and VL genes from human synovial B-cell hybridomas and synovial tissue demonstrates somatic mutations due to antigen activation. The FDC formations in the synovial tissue may therefore serve as an environment for B-cell maturation, which is involved in the generation of autoantibodies. An …
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Monoclonal Analysis of Spontaneous and Induced Antibody Responses in Autoimmune New Zealand Mice
… in autoimmune BW mice was facilitated by hybridoma technology. Various immunochemical and physicochemical parameters of generated monoclonal antibodies were examined to provide a qualitative functional and structural description of such etiologically distinct antibody repertoires in the …
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Structure, Function, and Diversity of Monoclonal Anti-Fluorescyl Antibodies
… a fluorescein hapten system in conjunction with hybridoma methodology. Monoclonal anti-fluorescyl IgG antibodies (nine clones) were produced by fusion of BALB/c splenocytes and Sp 2/O-Ag 14 myeloma cells. Analyses of these clones were performed in order to compare structure/function relationships …
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Production and Characterization of Monoclonal Antibodies against a Recombinant Nfa1 Protein Cloned from Pathogenic Naegleria fowleri
… were produced according to the protocol of hybridoma technique using myeloma cells (F0 cell). The McAbs were characterized by isotyping and Western blot analysis. RESULTS: Eight McAbs (A1F, A3C, A6D, A10F, C3A, C6F, C7F, B6B) against the rNfa1 protein were obtained. Eight McAbs isotypes were …
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Untersuchungen zu Bacillus cereus Enterotoxin-Komplexen auf zellulärer Ebene
… different cell lines (Vero-, Neuro2A-, Jurkat-, hybridoma cells) for their sensibility against culture supernatants of toxic B. cereus isolates, Vero cells proved to be the most sensitive. The sensitivity pattern of hybridoma cells differed from the other cell lines, as HBL containing …
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Design and testing of a high accuracy robotic single-cell manipulator
… be used to help select and isolate an individual hybridoma from polyclonal mixture of cells producing various types of antibodies. It is important to be able to do this cell-retrieval task since a single isolated hybridoma cell produces monoclonal antibody that only recognizes specific antigens, …
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Catalytic antibodies for amide cleavage and prodrug activation
… concerns the detection of catalytic activity in hybridoma supematants. A new method called "Capture"-CatELISA was developed, which allowed the efficient screening for catalysis of hundreds of hybridoma supematants in a very short period of time. To this end, a substrate Cl was developed, which …
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Herstellung, Expression und Charakterisierung von rekombinanten Antikörpern und Immuntoxinen gegen Phoma lingam, Sclerotinia sclerotiorum und Verticillium dahliae
… utilised for the classic antibody generation by hybridoma technology as well as for the generation of single-chain (scFv) presenting phage libraries. Recombinant scFv could be subsequently selected by a „Bio-panning” in the scope of the phage display technology. Additionally the bivalent …
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Genetic Investigation of MHC-independent Missing-self Recognition by NK Cells
… In the first results chapter, we generated hybridoma clones producing monoclonal antibodies against another inhibitory receptor in the Nkrp1 receptor family, NKR-P1G via the CELLISA assay. Our results demonstrate that CELLISA is a cost-effective, efficient, and high-throughput screening …
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The isolation of single chain variable region fragments (scFvs) from a phage display library, and expression of the isolated scFvs in Nicotiana benthamiana
… antibodies have been produced using hybridoma technology. They have also been successfully produced in other expression hosts such as bacteria, yeasts, insect cells and mammalian cell lines. However, these expression systems come with a few disadvantages, some of which include high …
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Developing Methods to Study Pathogenesis and Immune Response to HSV-2 in the Guinea Pig Model
… I generated and purified antibody from two Hybridoma lines; NG3.5 and VE-4. To generate enough antibody for immunological assays, I grew large quantities of antibody-producing hybridoma cells and collected the supernatant. From this, I isolated and purified the antibody for future assays. We …
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Developing Methods to Study Pathogenesis and Immune Response to HSV-2 in the Guinea Pig Model
… I generated and purified antibody from two Hybridoma lines; NG3.5 and VE-4. To generate enough antibody for immunological assays, I grew large quantities of antibody-producing hybridoma cells and collected the supernatant. From this, I isolated and purified the antibody for future assays. We …
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