Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Hyaluronidase"”.
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Formulation of Hyaluronidase Enzyme Sensitive Topical Nanomicrobicides for HIV Virus Transmission Prevention
… (HA NPs) can be achieved under the influence of hyaluronidase (HAase) enzyme. The Fractional Factorial Experimental Design (FFED) was employed to examine the formulation variables such as: molar concentrations of adipic acid dihydrazide (X₁) and 1-Ethyl-3-[3 dimethylaminopropyl]carbodiimide …
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The Substrate Specificity of Testicular Hyaluronidase and Its Use in Sequencing Chondroitin Sulfates
Testicular hyaluronidase was used to study the distribution of sulfated disaccharide units along chondroitin sulfate chains. The transglycosylation activity of the hyaluronidase was eliminated by use of excess levels of a second enzyme, (beta)-glucuronidase to remove all nonreducing terminal …
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The disintegration and dissolution of urinary calculi
… with a wetting agent, ficin, steapsin, and hyaluronidase as aides in the :in vitro" dissolution of calculi in "G"⁷ solution. This investigation indicated that a more effective solvent was needed.
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Extraction and Characterisation of Bioactive Compounds From New Zealand Black-Footed Abalone
… antioxidant, antibacterial, anticancer, anti-hyaluronidase, anti-collagenase activities, and collagen. Thus, this thesis aimed to explore the extraction of bioactive compounds from Zealand black-footed abalone using novel environmentally friendly extraction techniques in order to make it an …
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Bioproduction Optimization and Characterization of Hyaluronic Acid in Escherichia coli and Nicotiana benthamiana
… 2 (hHAS2) with and without the co-expression of hyaluronidase and were evaluated based on total HA yield and molecular weight. The engineered strains successfully produced HA with exceptionally low molecular weights (2.3–3.6 kDa) and narrow polydispersity index, indicating a highly uniform …
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Clostridium difficile and its toxins
… there is no proteolytic activity 6. that the hyaluronidase produced by all strains is antigenically uniform? and so is the lethal toxin; 7. and that the 'haemolysin' is almost certainly complex.
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Anti-aging potential of some freshwater microalgae extracts
… of aging enzymes, namely collagenase, elastase, hyaluronidase, and tyrosinase. UVB and UVA photoprotective activity of the microalgal extracts was also investigated. Antibacterial susceptibility testing was conducted on commensals, Propionibacterium acnes and Staphylococcus aureus. The results …
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The anticancer effects of the different analogs of cysteine deleted tachyplesin on A549 lung cancer cells
… analog in preventing cancer cell growth. Hyaluronidase and apoptosis assays were performed to better understand the mechanism by which the All D-CDT is preventing cancer cell survival. From this data it was seen that the All D-CDT analog inhibits cancer cell growth via a hyaluronan …
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Characterization of Glycyl-Sarcosine Uptake by Ovine Intestinal Brush Border Membrane Vesicles
… experiments determined that incubation in hyaluronidase is not necessary in order to separate mucosal tissue from the basement membrane and that the stop solution used in the uptake study needed to be buffered. Uptake was examined in proximal (denoted jejunal) and distal (denoted ileal) …
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Biochemical investigation into the toxic salivary secretion of the tick, Ornithodoros savignyi Audouin (1827)
… chromatography. One of the fractions showed weak hyaluronidase activity and was found to be homogeneous according to sedimentation equilibrium centrifugation. The molecular mass, determined by the latter method was found to be 9680. The amino acid composition of the fraction revealed, with a few …
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Hearing loss in experimental bacterial meningitis
… D39 deficient in pneumolysin, neuraminidase or hyaluronidase respectively. All animals developed a meningeal inflammatory response and a labyrinthitis. Hearing loss in pneumococcal meningitis was measured by recording the evoked auditory nerve compound action potential from the round window …
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Development and biophysical characterization of a hyaluronic acid – vitamin E conjugate as a subcutaneous delivery platform
… biodegradability by the endogenous enzyme hyaluronidase. In addition, HAtoco has shown the ability to double the in vitro release half-life of three model proteins (BSA, RNase, Lysozyme) and the in vivo release half-life of the clinical protein therapeutic Coversin when delivered …
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The Role of Surface Properties in the Transport of Folates Across the Intestine
… exposing tissue for different lengths of time to hyaluronidase. There was a marked association between the histological appearance of the glycocalyx and the uptake characteristics of folic acid. The present investigation has provided definitive evidence to support an important role of surface …
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Dietary regulation of myelopoiesis and stromal cell isolation
… nor did the addition of Elastase, TrypLE, Hyaluronidase, or Accutase. Similarly, common mechanical dissociation approaches also proved ineffective. However, the combination of gentle Dispase II + DNase dissociation with magnetic sorting dramatically enriched both ECs and MSCs. This work …
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THE ROLE OF HYALURONAN IN INNATE INTESTINAL DEFENSE
… by digestion of milk HA with a specific hyaluronidase. In addition, oral administration of human milk-derived HA to adult, wild-type mice resulted in induction of murine HßD2 ortholog in intestinal mucosa. Finally, treatment of cultured colonic epithelium with human milk HA preparations …
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In vitro model of periodontal epithelium
… treatment with phorbolmyristate acetate and hyaluronidase, and alteration of serum concentration in the culture medium. My model for junctional epithelium allowed the study of the effects of one possible periodontopathogen, Treponema denticola, on the epithelium. T. dent/cola caused rapid …
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Pancreatic Cancer: Oncomicrobes, Electric Fields, and Fluid Flow
… Our analysis of patients treated with PEGPH20, a hyaluronidase enzyme, revealed a transient reduction in velocity magnitudes one day post-treatment, though values generally returned to baseline by the conclusion of the dosing cycle. We observed substantial heterogeneity of velocity magnitudes both …
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Novel Preclinical Approaches to the Understanding and Treatment of Achilles Tendinopathy
… the therapeutic potential of a recombinant human hyaluronidase, rHuPH20 (biologic, FDA approved for reducing HA accumulation in tumors) in a novel Achilles tendinopathy and retrocalcaneal bursitis injury model. The potential of rHuPH20 to effectively clear the pro-inflammatory, HA-rich matrix …
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Hyaluronate Lyase Gene of <i>Streptococcus pyogenes</i>: Molecular Characterization and Regulation
… regarding the regulation of streptococcal hyaluronidase. As such, the expression of <em>hyl</em>A in the hyaluronate lyase-producing strain ATCC 10403 and in strains that do not produce detectable enzyme was analyzed by RT-PCR and quantitative PCR. Hyaluronate lyase transcript was detected …
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Physiological and Molecular Consequences of Large Y Chromosome Long Arm Deletions in Mice
… enzyme activity assays did not reveal altered hyaluronidase activity in MF1XYRIIIqdel sperm. Physiological differences between X- and Y- bearing sperm must result from differential gene expression, complicated by the syncitial nature of sperm development. To explore this, a detailed molecular …
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