Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Humanized mouse model"”.
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Development of a Humanized Mouse Model to Understand M.tb and HIV/M.tb Co-infection
… of death among HIV+ individuals. Several animal models are available to study TB infection although many do not reproduce disease pathology similar to humans. The human host tropism of HIV limits its study in animal models. The lack of a small animal model to study HIV/M.tb co-infection has …
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Gene Therapy and Stem Cell Therapy to Improve the Outcome of Human Islet Transplantation
… islets, was successful in immunodeficient animal models because the potent expressions of therapeutic proteins usually lead to an advantage in the post-transplantation survival of human islets. However, gene therapy provided limited success to overcome the immune rejection. Also gene therapy faced …
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Humanized Chimeric Receptors in the Therapy of Multiple Sclerosis
… autoimmune encephalomyelitis, the murine model of multiple sclerosis. We here demonstrate the ability of humanized chimeric receptors to suppress experimental autoimmune encephalomyelitis (EAE) in a humanized mouse model by redirecting T lymphocytes against autoreactive T-cells. The …
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TREATMENT AND MODELING OF EPSTEIN-BARR VIRUS POST-TRANSPLANT LYMPHOPROLIFERATIVE DISORDER
… antibody (α-EBNA-1 TCR-like mAb) using a mouse xenograft model of PTLD. With the aim of translating our findings into studies relevant to humans, we developed and optimized an autologous humanized mouse model for future preclinical evaluations of the antibody
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Pathology and mechanisms of increased M.tb growth and dissemination during co-infection with HIV-1
… we describe the development of the first animal model of TB/HIV co-infection using the human pathogens themselves. We have demonstrated increased pathology and bacterial growth previously seen during co- infection in humans. We have also demonstrated increased neutrophil infiltration during HIV …
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The Role of Inflammation in the Pathobiology of TB/HIV Co-infection
… to CD4+T cell loss. Using our newly developed humanized mouse model of TB/HIV co-infection, we find that initial acute co-infection in the lung is characterized by excessive inflammation. We observed greater pathology, inflammatory influx, and increased pro-inflammatory cytokines (e.g. IL-1, …
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The Role of Inflammation in the Pathobiology of TB/HIV Co-infection
… to CD4+T cell loss. Using our newly developed humanized mouse model of TB/HIV co-infection, we find that initial acute co-infection in the lung is characterized by excessive inflammation. We observed greater pathology, inflammatory influx, and increased pro-inflammatory cytokines (e.g. IL-1, …
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Dynamics of HIV-1 Infection and Therapy In Vivo
… new molecular tools to interrogate LICs in a humanized mouse model of HIV-1 infection.</p>
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Using machine learning to increase the predictive value of humanized mouse models for the human immune response to YFV-17D
Despite their utility as models for human systems, intrinsic differences between mouse and human biology limit direct translation of findings. Immunocompromised mice have been engrafted with functional human immune system components, or “humanized”, to better model the human immune response. …
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Determinants of Immunity against the Meningococcus
… nasal colonization I took advantage of a “humanized” mouse model of N. meningitidis nasal infection, wherein expression of human CEACAM1 allows N. meningitidis to colonize the murine nose. I reveal that protection against nasal colonization is absolutely reliant on B cells and neutrophils, …
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Engineering human hepatic tissue for modeling liver-stage malaria
… has been difficult due to a lack of human liver models that robustly recapitulate host-pathogen interactions in a physiologically relevant cell type. Through the application of hepatic tissue engineering concepts and techniques, this thesis sought to develop advanced models of liver-stage malaria …
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Lethal Hypermutation Induced by the Innate Cellular Restriction Factor APOBEC Destroys HIV-1 in Infected Humanized Mice
… able to evade APOBEC restriction in vivo exists. Humanized mice used as an in vivo model to study the capacity of APOBEC to restrict Vif-defective HIV revealed that the ability of the virus to replicate is severely crippled. HIV lacking Vif is heavily hypermutated by APOBEC, however, if the virus …
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Putative Roles of Cd200 In The Leukemogenesis and Immune Evasion of Leukemia Stem Cells
… and characterized two isogenic cell line models of CD200 expression. Utilizing these cells, we investigated the effects of CD200+ leukemia on the effector functions of isolated T cells, NK cells, and macrophages <em>in vitro</em>. To study CD200 in a physiological setting, we established a …
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Omics Approaches to Uncover Germline and Somatic Variation Underlying Inherited Sarcomagenesis
… a comparative genomics approach, leveraging a “humanized” mouse model of LFS with a hotspot mutation, <em>Trp53<sup>R172H</sup>,</em> analogous to <em>TP53<sup>R175H</sup></em> in humans. Hypothesizing that sarcoma etiology is similar in humans and mice, we then catalogued recurrent changes in …
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Mechanisms of Innate Immune Dysfunction During Mycobacterium tuberculosis and HIV Co-infection
… during in vivo TB and TB/HIV co-infection models were observed. Additionally, this work identified a novel role for the CLR Macrophage Galactose-type Lectin-1 (MGL-1) in a mouse model (C57BL/6 and MGL-1-/-) of experimental tuberculosis. Murine macrophages upregulated MGL-1 following in …
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Reprogramming the immune environment in breast cancer via dendritic cells.
… tumor growth and interleukin 13 production in a humanized mouse model of breast cancer. Thus, breast cancer cell-derived TSLP, by inducing OX40L expression on DCs, contributes to the Th2 immunity conducive to breast tumor development. In order to reprogram the inflammatory pro-tumor Th2 (iTh2) …
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Novel Antimalarial Compounds from the Optimization of the Malaria Box
… in vitro, no efficacy was observed in a humanized mouse model or a P. berghei infected mouse in vivo. In order to block potential metabolic spots and to probe for steric demand, a series of analogous featuring C1-deuteration, methyl substitution on B- and C-ring, and an ethylene bridge …
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Humanized Mouse with a Diverse Polyclonal B Cell Repertoire as a Model for Neutralizing Antibody Responses against EBV and HIV
… However, it has been historically difficult to model human neutralizing B cell responses to these two viruses using small animals. I leveraged a transgenic mouse model that encodes a genetically human antibody repertoire to generate neutralizing monoclonal antibodies (mAbs) against the EBV gp350 …
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The Ontogeny of Vaccine-Induced HIV-1 Glycan-Reactive Antibodies
… the characterization and immunization of a humanized mouse model bearing the CH01 V1V2-glycan bnAb unmutated common ancestor antibody; i.e. the B cells in these mice bear the CH01 antibody heavy and light chain sequences that were predicted to be germline-encoded prior to somatic …