Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Human whole blood"”.
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IN-VITRO PK/PD PROFILING AND MODELING OF THE ANTI-SICKLING AGENTS, 5-HYDROXYMETHYL FURFURAL (5-HMF) AND NOVEL SYNTHETIC ALLOSTERIC EFFECTORS OF HEMOGLOBIN (AEH) IN HUMAN WHOLE BLOOD
… dehydrogenase (ALDH) in the liver and red blood cells (RBC). Methods. Biopharmaceutical assessments were made for AEH, using calculated physicochemical properties. Their in-vitro hepatic metabolism (mediated by ALDH) was characterized using hepatic cytosol, and in-vitro-in-vivo …
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Effects of Graphene Oxide in vitro on DNA Damage in Human Whole Blood and Peripheral Blood Lymphocytes from Healthy individuals and Pulmonary Disease Patients: Asthma, COPD, and Lung Cancer
… there are concerns about their genotoxicity on human DNA. Published genotoxicity studies on GO NMs were performed using non-commercial GO with 2-3 layers of GO sheets, synthesized in various laboratories with the potential for inter-laboratory variabilities. However, what has not been studied …
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Effects of Graphene Oxide in vitro on DNA Damage in Human Whole Blood and Peripheral Blood Lymphocytes from Healthy individuals and Pulmonary Disease Patients: Asthma, COPD, and Lung Cancer
… there are concerns about their genotoxicity on human DNA. Published genotoxicity studies on GO NMs were performed using non-commercial GO with 2-3 layers of GO sheets, synthesized in various laboratories with the potential for inter-laboratory variabilities. However, what has not been studied …
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Measurement of blood analytes in turbid biological tissue using near-infrared Raman spectroscopy
Concentrations of blood constituents provide diagnostic information in clinical procedures. Among the medical community, there is great interest in developing an optical method of concentration measurements that eliminates reagents required in the current clinical chemistry techniques and is …
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An unbiased proteomic approach to identifying cell surface markers in neutrophils
… patterns’ have yet to be identified. Methods: Human whole blood samples were obtained from healthy volunteers. Neutrophils were isolated and subjected to a temperature shift to facilitate a wave of synchronised apoptosis. The plasma membrane proteome was assessed using tandem-mass-tag liquid …
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Protein FOG at the interface between G streptococci and human host defence lines
… may be of four different species and may infect humans and also animals. S. dysgalactiae equisimilis most commonly cause human GGS infections. These bacteria are part of the normal flora, but can cause pharyngitis, erysipelas and impetigo. In the immunocompromised host severe conditions, such as …
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Applications of LC-MS/MS in forensic toxicology for the analysis of drugs and their metabolites
… the simultaneous determination of 24 opioids in human whole blood, including, for the first time in human whole blood, naloxone-3-glucuronide. Although a large number of drugs of interest were included in the method, acceptance criteria for linearity, precision, and recovery for all analytes were …
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Microfluidic Approaches to Thrombosis and Hemostasis: Towards a Patient-Specific Test of Antiplatelet Therapeutics and the Assessment of Coagulopathy in Hemophilic and Trauma Patients
… and coagulation using minimum volumes of human whole blood. This dissertation describes the application of microfluidic assays, the manipulation of surface-patterned procoagulant and sub-endothelial proteins, anti-coagulation, and flow conditions to investigate platelet function and …
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The role of staphylococcal superantigen-like protein 7 (SSL7) in immune evasion
… lactis enhanced survival of the bacteria in a human whole blood killing assay. Moreover, in an in vitro migration assay and in vivo murine model of peritoneal inflammation, SSL7 inhibited the chemotaxis of inflammatory cells in response to a pathogenic stimulus. Protection against blood killing …
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Measurement and mechanisms of complement-induced neutrophil dysfunction
… Neutrophils were isolated from healthy volunteer blood and functions (priming, phagocytosis and reactive oxygen species production) were assessed using light microscopy, confocal microscopy and flow cytometry. A new assay was developed using an Attune Nxt™ acoustic focusing cytometer (Life …
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Interaktionen von Mikroorganismen und Granulozyten mit Polymer-Oberflächen
… adherence, vitality, interleukin production) in human whole blood, and the determination of microbial adherence onto polymer surfaces as a parameter for estimating the infection riskis possible. The investigation of the expression of various surface molecules after contact with six different …
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THE DESIGN OF A NOVEL ANTI-TUMOUR DRUG DELIVERY SYSTEM USING IMMUNE-DERIVED CELLS.
… exudate. The isolation of monocytes from human whole blood was also conducted since it would be necessary to carry out further studies in a human model. The number of unelicited murine peritoneal macrophages available for injection was maximised by culture of the macrophages in polyallomer …
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Readout of diagnostic assays on a centrifugal microfluidic platform
… (CV = 3%), and alcohol <br>(CV = 4%) in human whole blood. <br>The measurement of the hematocrit as an important marker for <br>medical diagnostics is transferred to the centrifugal microfluidic platform. <br>Here, a radially aligned dead end channel is first bubble-free <br>primed with …
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The immune-modulating activity of Sutherlandia frutescens
… in vitro using cytokines as biomarkers. Whole blood cells containing various concentrations of Sutherlandia frutescens were stimulated in vitro with either Lipopolysaccharide (LPS) or Phytohaemagglutinin (PHA). Results show that Sutherlandia frutescens is not toxic at any of the …
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The in vitro effects of heavy metals and nanoparticles on the immune system
… The murine macrophage cell line RAW 264.7 and human whole blood cell cultures (WBCs) were selected as immune system representatives to assess the effects of heavy metals and nanoparticles on the immune system. The effects of heavy metals and NPs on RAW cells were monitored either in the absence …
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The use of whole blood cell cultures as a model for assessing the effects of SeptilinTM on the immune system.
In the past three decades there has been a huge increase in the use of herbal medicine globally. The active principles of these herbal medicines are mostly unknown with supportive evidence for safety and efficacy very rare. SeptilinTM is a phytopharmaceutical formulation which is recommended for …